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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytotoxicity,Liposomes,Eptifibatide,Materials Testing
- چکیده:
- چکیده انگلیسی: Background: Eptifibatide (Integrilin®) is a hepta-peptide drug which specifically prevents the aggregation of activated platelets. The peptide drugs are encapsulated into nanolipisomes in order to decreasing their side effects and improving their half-life and bioavailability.
Objectives: In this study, the in vitro cytotoxicity and hemocompatibility of RGD-modified nano-liposomes (RGD-MNL) encapsulated a highly potent antiplatelet drug (eptifibatide) was investigated.
Material and Methods: RGD-MNL encapsulated eptifibatide was prepared using lipid film hydration and freeze/thawing method. The morphology and size distribution (about 90 nm) of RGD-MNL were characterized using transmission electron microscopy (TEM). The in-vitro cytotoxicity of nano-liposomes was examined using the MTT, LDH release and reactive oxygen species (ROS) generation assays. The effect of RGD-MNL on red blood cells (RBC) was investigated using hemolysis and LDH release assays.
Results: The results revealed that RGD-MNL had no significant cytotoxic effect on HeLa and HUVEC cell lines, and also no ROS generation increase in the cells. In addition, the adverse effect of RGD-MNL on LDH release and membrane integrity of RBC was not observed.
Conclusions: In conclusion, the recommended RGD-MNL formulations have not any significant cytotoxicity on normal cells or RBC and have potential for protecting and enhancing the activity of antiplatelet drugs.- انتشار مقاله: 29-07-1396
- نویسندگان: Hassan Bardania,Seyed Abbas Shojaosadati,Farzad Kobarfard,Dina Morshedi,Farhang Aliakbari,Mohammad Taher Tahoori,Elahe Roshani
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Journal of Chemistry-Section A
- نوع مقاله: Journal Article
- کلمات کلیدی: Sandal Santalum album,Biosorption,Mitigation,Frendlich isotherm
- چکیده:
- چکیده انگلیسی: In this study, mitigation of Cu(II) from aqueous solutions was evaluated using the sandal Santalum Album as biosorbent. The removal capacity of the Cu(II) was studied in batch experiments including such as initial metal concentration (25-400 mg/L), biosorbent dose (0.05-0.2 g), effects of contact time (0-1440 min), kinetics and pre-treatment of the biosorbent. The maximum removal of Cu(II) was obtained at the optimum pH of 5. The maximum equilibrium uptake for Cu(II) was Ba(OH)2-SA(248.8 mg/g-1) at 400 ppm. Kinetics studies explained that, mitigation of the Cu(II) was very fast from 0 to 120 min and achieved equilibrium at 240 min. The equilibrium was well explained by Frendlich isotherm. Kinetics was well fitted to pseudo second order equation with high R2 values.
- انتشار مقاله: 23-06-1398
- نویسندگان: Qadeer Ul Hassan,Adil Munir,Asad Bashir,Farhan Ali,Rana Muhammad Shahid
- مشاهده