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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Isolation,identification,Uranium,sulfur-oxidizing bacterium
- چکیده:
- چکیده انگلیسی: Bioleaching is the extraction of metals from their ores through the use of microorganisms. In
this process, the use of native bacteria leads to achieve more yields of metals. So, in the present
study, native sulfur-oxidizing bacterium in potentiality of uranium extraction was isolated from
Ghachin mine in Iran and identified by partial gene sequencing. For this purpose, the water
samples were collected from Ghachin mine and cultivated in Starkey medium. In following, the
isolate was inoculated into individual Starkey plates and incubated until the colonies indicating
the purified bacterium appeared. Then, the identification was carried out based on phenotypic
characteristics and 16s rDNA sequencing. After that, bioleaching of uranium experiments
carried out using uranium ore at 2.5 and 5% pulp densities. The result showed that after 15 days
of incubation, the bacteria in the fresh samples was grown. Following 5-7 days of the plate's
incubation, we obtained the single purified colonies of the bacteria. On the basis of 16s rDNA
nucleotide sequencing, the bacteria showed 99.71% similarity to A. thiooxidans ATCC 19377.
Besides, the bioleaching experiments indicated that the bacterium is capable of uranium
extraction in 2.5 and 5% pulp densities during 3 and 5 days. In conclusion, in this study, for the
first time, we isolated the native sulfur-oxidizing bacterium capable of uranium extraction, from
uranium mine of Gachin in Bandar Abbas, Iran.- انتشار مقاله: 17-12-1393
- نویسندگان: Faezeh Fatemi,Abbas Rashidi,Samaneh Jahani
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Isolation,identification,Uranium,sulfur-oxidizing bacterium
- چکیده:
- چکیده انگلیسی: Bioleaching is the extraction of metals from their ores through the use of microorganisms. In
this process, the use of native bacteria leads to achieve more yields of metals. So, in the present
study, native sulfur-oxidizing bacterium in potentiality of uranium extraction was isolated from
Ghachin mine in Iran and identified by partial gene sequencing. For this purpose, the water
samples were collected from Ghachin mine and cultivated in Starkey medium. In following, the
isolate was inoculated into individual Starkey plates and incubated until the colonies indicating
the purified bacterium appeared. Then, the identification was carried out based on phenotypic
characteristics and 16s rDNA sequencing. After that, bioleaching of uranium experiments
carried out using uranium ore at 2.5 and 5% pulp densities. The result showed that after 15 days
of incubation, the bacteria in the fresh samples was grown. Following 5-7 days of the plate's
incubation, we obtained the single purified colonies of the bacteria. On the basis of 16s rDNA
nucleotide sequencing, the bacteria showed 99.71% similarity to A. thiooxidans ATCC 19377.
Besides, the bioleaching experiments indicated that the bacterium is capable of uranium
extraction in 2.5 and 5% pulp densities during 3 and 5 days. In conclusion, in this study, for the
first time, we isolated the native sulfur-oxidizing bacterium capable of uranium extraction, from
uranium mine of Gachin in Bandar Abbas, Iran.- انتشار مقاله: 17-12-1393
- نویسندگان: Faezeh Fatemi,Abbas Rashidi,Samaneh Jahani
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Isolation,identification,Uranium,sulfur-oxidizing bacterium
- چکیده:
- چکیده انگلیسی: Bioleaching is the extraction of metals from their ores through the use of microorganisms. In
this process, the use of native bacteria leads to achieve more yields of metals. So, in the present
study, native sulfur-oxidizing bacterium in potentiality of uranium extraction was isolated from
Ghachin mine in Iran and identified by partial gene sequencing. For this purpose, the water
samples were collected from Ghachin mine and cultivated in Starkey medium. In following, the
isolate was inoculated into individual Starkey plates and incubated until the colonies indicating
the purified bacterium appeared. Then, the identification was carried out based on phenotypic
characteristics and 16s rDNA sequencing. After that, bioleaching of uranium experiments
carried out using uranium ore at 2.5 and 5% pulp densities. The result showed that after 15 days
of incubation, the bacteria in the fresh samples was grown. Following 5-7 days of the plate's
incubation, we obtained the single purified colonies of the bacteria. On the basis of 16s rDNA
nucleotide sequencing, the bacteria showed 99.71% similarity to A. thiooxidans ATCC 19377.
Besides, the bioleaching experiments indicated that the bacterium is capable of uranium
extraction in 2.5 and 5% pulp densities during 3 and 5 days. In conclusion, in this study, for the
first time, we isolated the native sulfur-oxidizing bacterium capable of uranium extraction, from
uranium mine of Gachin in Bandar Abbas, Iran.- انتشار مقاله: 17-12-1393
- نویسندگان: Faezeh Fatemi,Abbas Rashidi,Samaneh Jahani
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Isolation,identification,Uranium,sulfur-oxidizing bacterium
- چکیده:
- چکیده انگلیسی: Bioleaching is the extraction of metals from their ores through the use of microorganisms. In
this process, the use of native bacteria leads to achieve more yields of metals. So, in the present
study, native sulfur-oxidizing bacterium in potentiality of uranium extraction was isolated from
Ghachin mine in Iran and identified by partial gene sequencing. For this purpose, the water
samples were collected from Ghachin mine and cultivated in Starkey medium. In following, the
isolate was inoculated into individual Starkey plates and incubated until the colonies indicating
the purified bacterium appeared. Then, the identification was carried out based on phenotypic
characteristics and 16s rDNA sequencing. After that, bioleaching of uranium experiments
carried out using uranium ore at 2.5 and 5% pulp densities. The result showed that after 15 days
of incubation, the bacteria in the fresh samples was grown. Following 5-7 days of the plate's
incubation, we obtained the single purified colonies of the bacteria. On the basis of 16s rDNA
nucleotide sequencing, the bacteria showed 99.71% similarity to A. thiooxidans ATCC 19377.
Besides, the bioleaching experiments indicated that the bacterium is capable of uranium
extraction in 2.5 and 5% pulp densities during 3 and 5 days. In conclusion, in this study, for the
first time, we isolated the native sulfur-oxidizing bacterium capable of uranium extraction, from
uranium mine of Gachin in Bandar Abbas, Iran.- انتشار مقاله: 17-12-1393
- نویسندگان: Faezeh Fatemi,Abbas Rashidi,Samaneh Jahani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Medicinal plants and By-product
- نوع مقاله: Journal Article
- کلمات کلیدی: Liver enzymes,essential oil,hepatotoxicity,Carum carvi,Iron Oxide Nanoparticles,Detoxification enzymes
- چکیده:
- چکیده انگلیسی: Recently, there has been a great deal of interest in the use of the medicinal plants with high antioxidant compounds for curing liver injuries induced by hepatoxcitic agents. This study was to assess the protective effect of Carum carvi L. essential oil (E.O) on the hepatoxitic rats induced by overdose of iron oxide nanoparticles (NPs). The rats were distributed to 4 groups. In negative control group (NC), the rats received normal saline and DMSO daily for 3 days. In control group (C), iron oxide nanoparticles (Fe2O3) (200 mg/kg b.w) was injected daily for 3 days. In the treatment groups, iron oxide nanoparticles plus E.O at 100 & 200 mg/kg b.w were injected daily for 3 days. In following, cytochrome P450 (CYP450), glutathione S-transferase (GST), glutathione (GSH), aspartate transaminase (AST), alkaline phosphatase (ALP) and alanine transaminase (ALT) were estimated at 72 h after NP-treatment. The administration of the E.O could return considerably the decrease of the GST and GSH levels as well as the increase of the level of AST induced by iron oxide NPs. These results are in agreement with histopathological results. The data indicated that E.O provided an efficient prevention against iron oxide NPs-induced hepatotoxicity in rats.
- انتشار مقاله: 15-11-1396
- نویسندگان: Abolfazl Dadkhah,Faezeh Fatemi,Mohammad Reza Mohammadi Malayeri,Fatemeh Torabi,Mostafa Sarbazi,Salome Dini
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Medicinal plants and By-product
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,hepatotoxicity,Antioxidant parameters,Antioxidant agents
- چکیده:
- چکیده انگلیسی: Over loading of iron oxide nanoparticles can causes the liver injury through overproduction of free radicals. Zataria multiflora Boiss. (Lamiaceae) has been used for many years in folk medicine due to its antioxidant and antibacterial activities. This study evaluates -for the first time- the effect of Z. multiflora essential oil (EO) against iron oxide nanoparticles hepatotoxicity in rat model. Male Wistar rats were divided randomly into 4 groups; control group (C), non-treated control group (NT), treatment groups, nanoparticles plus Z. multiflora EO at 100 and 200 mg/kg b.w. In following, the liver and blood tissue were collected for estimating antioxidant / oxidative stress parameters including lipid peroxidation (LP), glutathione (GSH), glutathione-S-transferase (GST), and liver enzyme asparate transaminase (AST) and also histopathological examinations. Iron oxide nanoparticles toxicity produced a significant increase in the levels of LP, ferric reducing ability of plasma (FRAP), and AST activity concomitant with decrease in the levels of GSH and GST activity compared to the control group. However, in the treatment groups received Z. multiflora EO, the levels of LP and AST activity reduced together with increasing in GSH content and GST activity were significantly reported. Histopathological studies also supported the biochemical assessments indicating hepatic improvements induced by nanoparticles. Z. multiflora EO protected the liver from injuries induced by iron oxide nanoparticles which can be correlated with its antioxidant activities. This can be a valuable candidate in modulating the oxidative hepatic injuries.
- انتشار مقاله: 01-03-1396
- نویسندگان: Hamid Reza Attaran,Faezeh Fatemi,Azadeh Rasooli,Abolfazl Dadkhah,Mohammad Reza Mohammadi Malayeri,Salome Dini
- مشاهده