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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Glioma,IDH1 gene,DNA Sequencing,PCR–RFLP,immunuhistochemistry
- چکیده:
- چکیده انگلیسی: Background: IDH1 mutation shows diagnostic, prognostic, and predictive value in gliomas. Direct Sanger sequencing is considered the gold standard to detect IDH1 mutation. However, this technology is not available in most neuropathological centers in developing countries such as Indonesia. Immunohistochemistry (IHC) and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) have also been used to detect IDH1 mutation. This study aimed to compare DNA sequencing, IHC, and PCR–RFLP in detecting IDH1 mutations in gliomas. Methods: Research subjects were recruited from Dr. Sardjito Hospital. Genomic DNA was extracted from fresh or formalin-fixed paraffin-embedded samples of tumor tissue. DNA sequencing, PCR–RFLP and IHC were performed to detect IDH1 mutation. Sensitivity, specificity, and accuracy of PCR–RFLP and IHC were calculated by comparing them to DNA sequencing as the gold standard. Results: Among 61 recruited patients, 13 (21.3%) of them carried a mutation in codon 132 of the IDH1 gene, as shown by DNA sequencing. PCR–RFLP and DNA sequencing have a concordance value of 100%. Meanwhile, the concordance value between IDH1 R132H IHC and DNA sequencing was 96.7%. The sensitivity, specificity, positive predictive values, negative predictive values, and accuracy for PCR–RFLP were all 100%. On the other hand, the sensitivity, specificity, and accuracy of IHC were 92.3%, 97.9%, and 96.7%, respectively. Conclusion: This study showed that both PCR–RFLP and IHC have high accuracy in detecting IDH1 mutation. We recommend a combination of PCR–RFLP and IHC to detect IDH1 mutation in resource-limited settings.
- انتشار مقاله: 11-03-1399
- نویسندگان: Rusdy Ghazali Malueka,Emilia Theresia,Fitria Fitria,Ibnu Widya Argo,Aditya Dwi Donurizki,Sabillal Shaleh,Meutia Rizki Innayah,Adiguno Suryo Wicaksono,Kusumo Dananjoyo,Ahmad Asmedi,Rachmat Andi Hartanto,Ery Kus Dwianingsih
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Prognosis,Glioma,Indonesia,IDH,clinicopathological features
- چکیده:
- چکیده انگلیسی: Background: Gliomas remain one of the most common primary brain tumors. Mutations in the isocitrate dehydrogenase (IDH) gene are associated with a distinct set of clinicopathological profiles. However, the distribution and significance of these mutations have never been studied in the Indonesian population. This study aimed to elucidate the association between IDH mutations and clinicopathological as well as prognostic profiles of Indonesian patients with gliomas. Methods: In total, 106 patients with gliomas were recruited from a tertiary academic medical center in Yogyakarta, Indonesia. Formalin-fixed paraffin-embedded and fresh tissue specimens were obtained and sectioned for hematoxylin-eosin staining and immunohistochemical examinations. Genomic DNA was isolated and analyzed for the presence of IDH mutations using standard polymerase chain reaction and nucleotide sequencing methods. Clinicopathological data were collected from medical records. Results: Although no IDH2 mutation was identified, IDH1 mutations were found in 23 (21.7%) of the patients. Patients with IDH1 mutations tended to have a history of smoking and a shorter interval between onset of symptoms and initial surgical interventions. Frontal lobe involvement, oligodendroglial histology, lower Ki67 expression, WHO grades II and III gliomas, and methylated O6-methylguanine-DNA methyltransferase (MGMT) promoters were significantly associated with the presence of IDH1 mutations. Compared with patients with IDH1-wild-type, patients with IDH1 mutation were observed to have a longer overall survival. Conclusions: IDH1 mutations are associated with certain clinicopathological and prognostic profiles in Indonesian patients with gliomas. This finding demonstrates the importance of identifying IDH mutations as part of the management of patients with glioma in Indonesia.
- انتشار مقاله: 01-02-1399
- نویسندگان: Rusdy Ghazali Malueka,Ery Kus Dwianingsih,Halwan Fuad Bayuangga,Andre Stefanus Panggabean,Ibnu Widya Argo,Aditya Dwi Donurizki,Sabillal Shaleh,Adiguno Suryo Wicaksono,Kusumo Dananjoyo,Ahmad Asmedi,Rachmat Andi Hartanto
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Survival,Breast carcinoma,Luminal and non-luminal subtype,lymphangiogenesis,VEGF-C
- چکیده:
- چکیده انگلیسی: Background: Breast carcinomas (BCs) are sub-classified according to the molecular characteristics into luminal and
non-luminal subtypes that clinically show different biological behavior, treatment and prognosis. BCs spread primarily
through lymphatic vessels using cascade processes of lymphagiogenesis in which VEGF-C plays an important role during
lymph node metastasis. Prognostic value of VEGF-C in luminal and non-luminal BC is still unclear and has not been
studied thoroughly to clarify and define prognosis and therapeutic monitoring. Aim: To define the prognostic value of
lymphangiogenesis on survival rates of luminal and non-luminal subtypes BC. Materials and Methods: This study
applied prospective cohort design, using 130 patients of invasive duct carcinoma of the breast, stage I-IIIA, from
Sardjito General Hospital, Indonesia and subsequent longitudinal follow-up. Immunohistochemical staining was
carried out using anti-ER, -PR, -Her-2, VEGF-C, VEGFR-3 and D2-40 antibodies. The related clinicopathologic
characteristics of BC patients and lymphangiogenesis determinants, including VEGF-C expression, were statistically
analyzed. Results: In non-luminal BC subtypes, VEGF-C expression (HR=0.04; 95% CI=0.01-0.41), lymph node
metastasis (HR=0.14; 95% CI=0.04-0.55) and stage (HR=0.30; 95% CI= 0.02-0.76) were determined as independent
prognostic factors on survival rates. However, the lymphangiogenesis determinants were not associated with the survival
rates of luminal BC subtypes. Conclusion: This study suggested that lymphangiogenesis affects survival rates of
non-Luminal subtype rather than the luminal subtypes of BC.- انتشار مقاله: 12-06-1396
- نویسندگان: Irianiwati Widodo,Ery Kus Dwianingsih,Totok Utoro,Sumadi Lukman Anwar,Teguh Aryandono,Soeripto .
- مشاهده