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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,IgG Avidity Test,Ocular Toxoplasmosis
- چکیده:
- چکیده انگلیسی: Background: The diagnostic methods which are used for acute ocular toxoplasmosis are very important; if the treatment is delayed, it sometimes leads to loss of vision. Fewstudies have been performed to evaluate serological tests used in the diagnosis of acute ocular toxoplasmosis. Objective: To evaluate the immunoglobulin (Ig) M, G and IgG avidity tests for diagnosis of acute ocular toxoplasmosis in the northeast of Iran. Methods: A cross-sectional study was carried out from January 2014 to December 2016. After an opthalmic examination was conducted by a retina specialist, 16 typical acute and 34 typical chronic ocular toxoplasmosis cases were included in this study. Information on clinical manifestations, age and occupation was recorded. Anti-Toxoplasma IgG, IgM and IgG avidity tests were administered on serum samples using the ELISA method. Results: Blurring of vision in all patients was the most clinical presentation. The IgG avidity test could diagnose all acute and recent cases. However, three false positive and one false negative result occurred using the IgM test by ELISA. The false negative result in all likelihood occurred because the patient was at the beginning stage of the infection. Conclusion: The result of this study showed that IgM is not a reliable marker of acute disease. Repetition of the serology tests was proposed in cases with clinical manifestations without detectable antibody titer after approximately two weeks. IgG avidity testing results coincided with clinical diagnosis and it could therefore considered to be a reliable method to differentiate between recently acquired and chronic ocular toxoplasmosis.
- انتشار مقاله: 22-11-1396
- نویسندگان: Elham Moghaddas,Seyedeh Maryam Hosseini,Karim Sharifi,Abdolrahim Rezai,Saman Soleimanpour,Mohammad Mobin Miri Moghaddam,Seyed Aliakbar Shamsian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Leishmania infantum,Pelargonium roseum,Promastigote,Glucantime
- چکیده:
- چکیده انگلیسی: Background and aims: Visceral leishmaniasis so called Kala-azar is one of the important health care problems in the world. Some herbal drugs are noticed because of toxicity and drug resistance against pentavalent antimonials as used in treatment of Kala-azar. In this study, anti-leishmanial activity of Pelargonium roseum Essential oil on the in vitro growth of Leishmania infantum promastigotes in comparison to Glucantime was studied.
Methods: Different concentrations of Pelargonium roseum essential oil on culture of Leishmania infantum promastigote were surveyed with Anti-leishmania activity assays at 24 hr and 48 hr at 570 nm wavelength. All the data were analyzed by using SPSS version and anti-leishmanial potency of the oil extract of Geranium in different concentration were compared with standard dose of Glucantime.
Results: All concentration in 24 hr showed no remarkable difference. 25, 50 and 100 µL/ml efficacy was same as Glucantime on the growth of promastigotes in 24 hr MTT. All concentrations except 1µL/ml in 48hr MTT had resemble impact on the growth of promastigotes. 5 µL/ml concentration of Pelargonium roseum essential oil was significant difference effect on the parasite (P= 0.043) in 48hr.
Conclusion: Pelargonium roseum essential oil doesn’t treat Leishmania infantum promastigote quickly. After 48 hr, 5 µL/ml concentration can be a suitable candidate in clinical trials.- انتشار مقاله: 04-07-1395
- نویسندگان: Mohaddeseh Abouhosseini Tabari,Elham Moghaddas,Bibi Razieh Hosseini Farash,Mohammad Amin Ebrahimi,Nilofar Nabavi Mousavi,Mohammad Reza Youssefi
- مشاهده