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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,anti-cancer activity,marine sponge,Stylissa carteri
- چکیده:
- چکیده انگلیسی: Objective: Despite advanced treatment options available, drug resistance develops in breast cancer (BC) patients
requiring novel effective drugs. Stylissa carteri, a marine sponge predominantly living in Indonesia territories, has
not been extensively studied as anti-cancer. Therefore, this study targeted to assess the anti-tumor activity of the
ethanol extract of S. carteri in BC cells. Methods: S. carteri was collected from Pramuka Island, at Kepulauan Seribu
National Park, Jakarta, Indonesia and extracted using ethanol. Different BC cells including MDA MB 231, MDA
MB 468, SKBR3, HCC-1954 and MCF-7 cells were treated with this extract for cytotoxic analysis using MTT assay.
Spheroid growth assay and apoptosis assay were conducted in HCC-1954 cells. In addition, cell migration analysis and
synergistic activity with doxorubicin or paclitaxel were conducted in MDA MB 231 cells. This extract was subjected
also for GC-MS analysis. Results: The results show that ethanol extract of S. carteri demonstrated a cytotoxic activity
in BC cells. The IC50 of this extract was lower 15 μg/ml in MDA MB 231, MDA MB 468, SKBR3, and HCC-1954
cells. Moreover, this extract inhibited spheroids growth and induced apoptosis in HCC-1954 cells. It inhibited cell
migration and demonstrated a synergistic activity with doxorubicin or paclitaxel on triggering cell death in MDA MB
231 cells. Furthermore, GC-MS analysis indicated that this extract contained 1,2-Benzenediol, Dibutyl phthalate and
9,12-Octadecadienoic acid, ethyl ester. Conclusion: Our preliminary data indicate a potential anti-tumor activity of
ethanol extract of S. carteri in breast cancer cells.- انتشار مقاله: 30-07-1397
- نویسندگان: Muhammad Hasan Bashari,Fathul Huda,Tamia S Tartila,Sarah Shabrina,Tenny Putri,Nurul Qomarilla,Harold Atmaja,Beginer Subhan,Ikhwan Resmala Sudji,Edy Meiyanto
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: 5-Fluorouracil,Cell cycle,NF-B,PGV-1,WiDr cells
- چکیده:
- چکیده انگلیسی:
Cell cycle regulation and the NF-κB pathway in cancer cells are important in mediating resistance to 5-Fluorouracil (5-FU). Pentagamavunon-1 (PGV-1), a curcumin analog, is known to exhibit stronger growth inhibitory effects than curcumin itself in several cancer cells. In this study, we evaluated the potency of PGV-1 in combination with 5-FU in WiDr colon cancer cells. In MTT assays, PGV-1 did not only exhibit stronger growth inhibitory effects than both 5-FU and curcumin, but also enhanced the cytotoxicity of 5-FU. Flow cytometry demonstrated that single treatments with PGV-1 and 5-FU resulted in different effects on cell cycle profiles. PGV-1 induced G2/M arrest while 5-FU caused S-phase arrest at low concentration (1 μM) and G1-phase arrest at high concentration (100 μM). Interestingly, the combination of 5-FU and PGV-1 enhanced cell accumulation in S-phase. Although a single treatment with either 5-FU or PGV-1 increased cyclin D1 at the protein level, the combination treatment resulted in significant suppression. In addition, PGV-1 inhibited activation of NF-κB and suppressed the expression of cyclooxygenase-2, an NF-κB downstream protein. In conclusion, PGV-1 increased the cytotoxic effect of 5-FU on WiDr cells through inhibition of NF-κB activation.- انتشار مقاله: 15-02-1396
- نویسندگان: Edy Meiyanto,Endah Puji Septisetyani,Yonika Arum Larasati,Masashi Kawaichi
- مشاهده