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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Otorhinolaryngology
- نوع مقاله: Journal Article
- کلمات کلیدی: Prevention,Antioxidant,myricetin,Noise-induced hearing loss
- چکیده:
- چکیده انگلیسی: Introduction:
Exposure to hazardous noise induces one of the forms of acquired and preventable hearing loss that is noise-induced hearing loss (NIHL). Considering oxidative stress as the main mechanism of NIHL, it is possible that myricetin can protect NIHL by its antioxidant effect. Therefore, the present study aimed to investigate the preventive effect of myricetin on NIHL.
Materials and Methods:
A total of 21 Wistar rats were randomly divided into five groups, namely (1) noise exposure only as control group, (2) noise exposure with the vehicle of myricetin as solvent group, (3) noise exposure with myricetin 5 mg/kg as myricetin 5 mg group, (4) noise exposure with myricetin 10 mg/kg as myricetin 10 mg group, (5) and non-exposed as sham group. The hearing status of each animal was assessed by Distortion Product Otoacoustic Emissions.
Results:
The levels of response amplitude decreased after the exposure to noise in all groups and returned to a higher level after 14 days of noise abstinence at most frequencies; however, the difference was not significant in the myricetin-receiving or control groups.
Conclusion:
The results of this study showed that two doses of myricetin (5 and 10 mg/kg) administered intraperitoneally could not significantly decrease transient or permanent threshold shifts in rats exposed to loud noise.- انتشار مقاله: 08-04-1397
- نویسندگان: Maryam Bahaloo,Mohammad Ebrahim Rezvani,Ehsan Farashahi Yazd,Mohammad Hossein Davari,Amir Houshang Mehrparvar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,Rats,Amygdala,CA1 region,Vanilloid receptor subtype 1
- چکیده:
- چکیده انگلیسی: Background: Chronic use of opioids usually results in physical dependence. The underlying mechanisms for this dependence are still being evaluated. Transient receptor potential vanilloid type 1 (TRPV1) are important receptors of pain perception. Their role during opioid dependence has not been studied well. The aim of this study was to evaluate the effect of morphine-dependence on the expression of TRPV1 receptors in the amygdala and CA1 region of the hippocampus. Methods: This study used four groups of rats. Two groups of rats (morphine and morphine+naloxone) received morphine based on the following protocol: 10 mg/kg (twice daily, 3 days) followed by 20, 30, 40 and 50 mg/kg (twice daily), respectively, for 4 consecutive days. Another group received vehicle (1 ml/kg) instead of morphine given using the same schedule. The morphine+naloxone group of rats additionally received naloxone (5 mg/kg) at the end of the protocol. The control group rats received no injections or intervention. The amygdala and CA1 regions of the morphine, saline-treated and intact animals were isolated and prepared for real-time PCR analysis. Results: Administration of naloxone induced withdrawal signs in morphine-treated animals. The results showed a significant decrease in TRPV1 gene expression in the amygdala (P<0.05) but not the CA1 region of morphine dependent rats. Conclusion: TRPV1 receptors may be involved in morphine-induced dependence.
- انتشار مقاله: 21-02-1393
- نویسندگان: Elham Hakimizadeh,Mohammad Kazemi Arababadi,Ali Shamsizadeh,Mohammad Allahtavakoli,Mohammad Ebrahim Rezvani,Ali Roohbakhsh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Anti-Oxidants,Superoxide Dismutase,NADPH Oxidase,Gene expression,myricetin,Noise-induced hearing loss
- چکیده:
- چکیده انگلیسی: Objective(s): Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.
Materials and Methods: Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.
Results: Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes.
Conclusion: The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects.- انتشار مقاله: 19-03-1398
- نویسندگان: Maryam Bahaloo,Mohammad Ebrahim Rezvani,Ehsan Farashahi Yazd,Fatemeh Zare Mehrjerdi,Mohammad Hossein Davari,Ali Roohbakhsh,Abolfazl Mollasadeghi,Haniyeh Nikkhah,Maryam Vafaei,Amir Houshang Mehrparvar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Anti-Oxidants,Superoxide Dismutase,NADPH Oxidase,Gene expression,myricetin,Noise-induced hearing loss
- چکیده:
- چکیده انگلیسی: Objective(s): Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.
Materials and Methods: Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.
Results: Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes.
Conclusion: The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects.- انتشار مقاله: 19-03-1398
- نویسندگان: Maryam Bahaloo,Mohammad Ebrahim Rezvani,Ehsan Farashahi Yazd,Fatemeh Zare Mehrjerdi,Mohammad Hossein Davari,Ali Roohbakhsh,Abolfazl Mollasadeghi,Haniyeh Nikkhah,Maryam Vafaei,Amir Houshang Mehrparvar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Memory,Berberine,Hippocampus,Vascular dementia
- چکیده:
- چکیده انگلیسی: Objective(s):The major objective of the present study was to investigate the potential neuroprotective effect of berberine chloride on vascular dementia. Berberine, as an ancient medicine in China and India, is the main active component derived from the Berberis sp. Several studies have revealed the beneficial effects of berberine in various neurodegenerative disorders.
Materials and Methods: To induce vascular dementia, chronic bilateral common carotid artery occlusion was performed on male Wistar rats. After surgery, the rats were treated daily by oral administration of berberine chloride (50 mg/kg) for two months. The cognition function of treated rats, were evaluated by Morris Water Maze (MWM) test. In addition, Nissl and TUNEL staining were chosen to assess neuronal damage within the hippocampal CA1 area.
Results: It was obvious that chronic cerebral hypoperfusion (CCH), caused cognitive impairment and neuronal damages within CA1 hippocampal subregion. Berberine chloride was able to prevent cognitive deficits, (P<0.05) and reversed CCH-induced hippocampal neuronal loss and apoptosis, (P<0.05).
Conclusion: Berberine chloride may be considered as a potential treatment for cognitive deficits and neuronal injury caused by CCH in the hippocampal CA1 area.- انتشار مقاله: 08-02-1396
- نویسندگان: Mahila Lotfi Aski,Mohammad Ebrahim Rezvani,Mehdi Khaksari,Zeynab Hafizi,Zeynab Pirmoradi,Somayeh Niknazar,Fatemeh Zare Mehrjerdi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Addiction,Cerebral ischemia,opiates,Embolic stroke,Morphine withdrawal syndrome
- چکیده:
- چکیده انگلیسی: Objective(s) Opioid abuse is still remained a major mental health problem, a criminal legal issue and may cause ischemic brain changes including stroke and brain edema. In the present study, we investigated whether spontaneously withdrawal syndrome might affect stroke outcomes. Materials and Methods Addiction was induced by progressive incremental doses of morphine over 7 days. Behavioral signs of withdrawal were observed 24, 48 and 72 hr after morphine deprivation and total withdrawal score was determined. Cerebral ischemia was induced 18-22 hr after the last morphine injection by placing a natural clot into the middle cerebral artery (MCA). Neurological deficits were evaluated at 2, 24 and 48 hr after ischemia induction, and infarct size and brain edema were determined at 48 hr after stroke. Results Morphine withdrawal animals showed a significant increase in total withdrawal score and decrease of weight gain during the 72 hr after the last morphine injection. Compared to the addicted and control animals, infarct volume and brain edema were significantly increased in the morphine deprived animals (P< 0.05) at 48 hr after cerebral ischemia. Also, neurological deficits were higher in the morphine-withdrawn rats at 48 hr after stroke (P< 0.05). Conclusion Our data indicates that spontaneous withdrawal syndrome may worsen stroke outcomes. Further investigations are necessary to elucidate mechanisms of opiate withdrawal syndrome on stroke.
- انتشار مقاله: 28-06-1394
- نویسندگان: Mohammad Allahtavakoli,Ruhollah Moloudi,Mohammad Ebrahim Rezvani,Ali Shamsizadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: essential oil,Heart,Asafoetida,Ischemic-reperfusion injury
- چکیده:
- چکیده انگلیسی: Objective: Previous studies reported that asafetida from Ferula assa-foetida Linn. species and its essential oil (AEO) have antioxidant effects. In the present study, the effect of AEO was evaluated on ischemic-reperfusion injury in isolated rat hearts.
Materials and Methods: Forty-eight male Wistar rats were divided into 6 groups: 1) control group, 2) vehicle group, 3-5) AEO groups and, 6) carvedilol group. In the control group, hearts were only subjected to 30-min global ischemia followed by 120-min reperfusion. Hearts in other groups were perfused with vehicle (Tween 0.1%), AEO (0.125, 0.25 or 0.50 µL/g heart) or carvedilol (10 µM) for 5 min immediately before the induction of ischemia.
Results: Compared to the control group, myocardial dysfunction was significantly more severe only in group 5 in which a significant increase in left ventricular end diastolic pressure and a significant decrease in left ventricular developed pressure and ±dp/dt. Also, the activities of lactate dehydrogenase and creatine kinase as the markers of myocardial injury were significantly higher only in group 5 compared to control group. The size of infarct and the incidence of irreversible fibrillation did not show any significant differences between the control group and groups 3-5.
Conclusion: These results showed that perfusion of isolated rat hearts with AEO 0.5 µL/g heart, but not at lower concentrations, might worsen myocardial ischemic-reperfusion injury.- انتشار مقاله: 20-05-1396
- نویسندگان: Hassan Esmaeili,Zahra Hafezimoghadam,Mansour Esmailidehaj,Mohammad Ebrahim Rezvani,Zeynab Hafizibarjin
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Seizure,Plant extracts,Zataria multiflora Boiss,Pentylenetetrazole
- چکیده:
- چکیده انگلیسی:
Objective: At present, there are many antiepileptic drugs with a wide range of side effects on the human body. It was assumed that Zataria multiflora Boiss (Z. multiflora) with sedative, anti-spasmodic and anti-inflammatory activity may be effective in the treatment of epilepsy. The aim of the present study was to elucidate the effect of Z. multiflora hydroalcoholic extract and its fraction extracts on pentylenetetrazole (PTZ)-induced chemical kindling.
Materials and Methods: In this experimental study, eight separate groups of male albino mice were used. All groups received 11 separate intraperitoneal injections of PTZ (35 mg/kg) with two-day intervals. 30 min before the injection of PTZ, mice received vehicle, Z. multiflora hydroalcoholic extract (300 and 600 mg/kg), n-hexane, acetone, methanol fraction extracts (150 mg/kg), or diazepam (10 mg/kg).
Results: The kindled mice that were pretreated with vehicle showed a gradual increase in their seizure scores up to the end of the study. The hydroalcoholic extract of Z. multiflora (300 and 600 mg/kg) reduced seizure scores significantly. However, n-hexane, acetone and methanol extracts did not affect seizure scores significantly.
Conclusion: The present findings demonstrate that the hydroalcoholic extract of Z. multiflora did reduce the severity of seizure attacks in PTZ-induced chemical kindling in mice.- انتشار مقاله: 06-05-1394
- نویسندگان: Ali Shamsizadeh,Farangis Fatehi,Fatemeh Arab Baniasad,Fatemeh Ayoobi,Mohammad Ebrahim Rezvani,Ali Roohbakhsh
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Pain,Salvigenin,Flavonoid,Salvia officinalis,Inflammation
- چکیده:
- چکیده انگلیسی: Background and aims: Inflammation is one of the defense mechanisms of body and unpleasant sensation of pain is caused by tissue damage. Mostly, inflammation occurs through the release of inflammatory mediators. Salvia officinalis is one of the most valuable medicinal kind of mint order. Salvigenin is one of the active flavonoids existing in this plant. The aim of this study was to evaluate the anti-inflammatory and analgesic effect of salvigenin, Salvia officinalis flavonoid extracted. Methods: In this laboratory experimental study, plant was extracted and the column chromatography was used to purify prepared extracts. 100 male albino mice and 48 male wistar rats were selected. In the hot plate test and in the writhing test, animals were divided randomly into 5 groups. Group 1 (received 10 mg/kg normal saline), groups 2, 3 and 4 (received Salvigenin 25, 50 and 100 mg/kg intraperitoneally, espectively), group 5 (received 10 mg/kg morphine in hot plate test and 10 mg/kg indomethacin in writhing test). In the inflammatory test, animals were divided into 6 groups. Group 1 was assigned as a control group which received 0.05 ml of carrageenin. Groups 2, 3 and 4 (received Salvigenin, at doses of 25, 50 and 100 mg/kg). Group 5 (received 10 mg/kg indomethacin) and then changes of the volume of all groups were measured. Data were analyzed using ANOVA and Tukey test and PResults: In writhing test, Salvigenin reduced the number of abdominal contractions at doses of 50 and 100 mg/kg. Increasing dose of Salvigenin, with reduction in abdominal cramps resulted in the increasing of pain inhibition, and the percentage of this inhibition was statistically significant (P<0.001). In hot plate test, also 30, 45 and 60 minutes after injection of Salvigenin and morphine showed significant difference compared to the control group (P<0.001). Also, Salvigenin increased the maximum percentage of analgesic compared to the control group (P<0.001). Salvigenin could reduce inflammation and in the group that received Salvigenin at 100 mg/kg, the inflammation was significantly lower than the control group (P<0.05). Discussion: Our findings showed that Salvigenin has dose-dependent analgesic effect so that it can be useful in controlling of inflammations, acute and chronic pain.
- انتشار مقاله: 10-02-1394
- نویسندگان: Amir Hossein Mansourabadi,Hamid Mohammad Sadeghi,Nastaran Razavi,Ebrahim Rezvani
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Pain,Salvigenin,Flavonoid,Salvia officinalis,Inflammation
- چکیده:
- چکیده انگلیسی: Background and aims: Inflammation is one of the defense mechanisms of body and unpleasant sensation of pain is caused by tissue damage. Mostly, inflammation occurs through the release of inflammatory mediators. Salvia officinalis is one of the most valuable medicinal kind of mint order. Salvigenin is one of the active flavonoids existing in this plant. The aim of this study was to evaluate the anti-inflammatory and analgesic effect of salvigenin, Salvia officinalis flavonoid extracted. Methods: In this laboratory experimental study, plant was extracted and the column chromatography was used to purify prepared extracts. 100 male albino mice and 48 male wistar rats were selected. In the hot plate test and in the writhing test, animals were divided randomly into 5 groups. Group 1 (received 10 mg/kg normal saline), groups 2, 3 and 4 (received Salvigenin 25, 50 and 100 mg/kg intraperitoneally, espectively), group 5 (received 10 mg/kg morphine in hot plate test and 10 mg/kg indomethacin in writhing test). In the inflammatory test, animals were divided into 6 groups. Group 1 was assigned as a control group which received 0.05 ml of carrageenin. Groups 2, 3 and 4 (received Salvigenin, at doses of 25, 50 and 100 mg/kg). Group 5 (received 10 mg/kg indomethacin) and then changes of the volume of all groups were measured. Data were analyzed using ANOVA and Tukey test and PResults: In writhing test, Salvigenin reduced the number of abdominal contractions at doses of 50 and 100 mg/kg. Increasing dose of Salvigenin, with reduction in abdominal cramps resulted in the increasing of pain inhibition, and the percentage of this inhibition was statistically significant (P<0.001). In hot plate test, also 30, 45 and 60 minutes after injection of Salvigenin and morphine showed significant difference compared to the control group (P<0.001). Also, Salvigenin increased the maximum percentage of analgesic compared to the control group (P<0.001). Salvigenin could reduce inflammation and in the group that received Salvigenin at 100 mg/kg, the inflammation was significantly lower than the control group (P<0.05). Discussion: Our findings showed that Salvigenin has dose-dependent analgesic effect so that it can be useful in controlling of inflammations, acute and chronic pain.
- انتشار مقاله: 10-02-1394
- نویسندگان: Amir Hossein Mansourabadi,Hamid Mohammad Sadeghi,Nastaran Razavi,Ebrahim Rezvani
- مشاهده