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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Applied Biotechnology Reports
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial,Moringa Oleifera,plants,Protease,Characteristics,Casein
- چکیده:
- چکیده انگلیسی: Introduction: Proteases are hydrolyzing enzymes and are considered to be one of the most important groups of enzymes for industry and are used in leather, pharmaceutical, and food industry along with detergents, and bioremediation processes. The main objectives of this study were (i) to extract, partially purify, and characterize the protease enzyme from Moringa (Moringa oleifera) leaves; and (ii) to investigate the effect of such an enzyme against some pathogenic bacteria.
Materials and Methods: This enzyme was extracted in a 0.1 M phosphate buffer pH 7. It was left for 24 hours in a refrigerator and was then filtered using filter paper Whatman no. 41. The aqueous filtrate was used to estimate the proteolytic activity.
Results: Purification by ammonium sulfate gave the best results at 50%-70% concentration which had the highest specific activity, highest purification fold with the percentage yield of 45.3%. Gel filtration by Sephadex G-100 gave the best specific activity and the best purification fold with the yield from fraction of 34%-43%. The protease enzyme has optimum pH 7 and temperature 50 oC. The enzyme was thermally stable at 40-70 oC for 20-30 minutes. Some metal ions were activator on the enzyme-like Mn2+ (highest), Ba2+, Ca2+, and Na+. The efficacy of protease enzyme was improved when integration with antibiotic against certain bacterial including Bacillus cereus (S3), Staphylococcus aureus, Salmonella typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes. Also, E. coli O157:H7, L. monocytogenes, and Yersinia enterocolitica did not show any growth at pH 10.
Conclusions: To conclude, it can be stated that protease enzyme can be considered as a promising agent, cheap, and safe source which is suitable for using in various industries.
- انتشار مقاله: 22-02-1399
- نویسندگان: Aya M. Abd-ElKhalek,Dina M. Seoudi,Osama A. Ibrahim,Nabil S. Abd-Rabou,Eman M. Abd ElAzeem
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: AML,GM-CSF,CEBPA,FLT3-ITD,NPM1 mutation A
- چکیده:
- چکیده انگلیسی: Background: Acute myeloid leukemia (AML) is characterized by clonal expansion of myeloid precursors with diminished capacity for differentiation. It develops as the consequence of a series of genetic changes in a hematopoietic precursor cell. Purpose This study aimed to investigate the correlation between GM-CSF gene expression and different molecular prognostic markers such as FLT3-ITD, NPM1 mutation A and CEBPA gene expression in 100 Egyptian AML patients. As well as, correlation with the response to induction therapy, DFS andOS in these patients. Methodology: Quantitative assessment of GM-CSF gene expression was performed by qRT-PCR. Additional prognostic molecular markers were determined as FLT3-ITD and NPM1 mutation A together with quantitative assessment of CEBPA gene expression by qRT-PCR. Results: Patients with high GM-CSF expression levels had better OS and DFS with p value 0.004 and 0.02, respectively. However, no statistically significant difference between low andhigh GM-CSF gene expression was found regarding the response to therapy (p value= 0.08). Most patients with low CEBPA expression had resistant disease together with poor OS and DFS (P value =
- انتشار مقاله: 18-11-1398
- نویسندگان: Bassant Nagdy,Hebatallah A Kassem,Abdel-Rahman B Abdel-Ghaffar,Dina M Seoudi,Neemat M Kassem
- مشاهده