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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Age of onset,Single Nucleotide Polymorphism,Breast carcinoma,FGFR2,p21
- چکیده:
- چکیده انگلیسی: Objectives: Breast cancer tend to be more progressive with poorer prognosis in younger patients than those at an older age. Single Nucleotide Polymorphisms (SNPs) of P53 Pro72Arg, MDM2 SNP309, P21 Ser31Arg, ER SNP594, HER2 Ile655Val, and FGFR2 rs2981582 have drawn attention as genetic factors associated with cancer risk. However, there were contradictory results involving different races and their association is still unknown in Indonesian populations. This study was performed to examine the proportion of these six genes polymorphisms and their associations with age of onset of breast cancer patients in Yogyakarta, Indonesia. Methods: Biorepository DNA from 199 patients registered at Dr. Sardjito Hospital Yogyakarta from 2006-2013 were tested for polymorphisms using the PCR-RFLP method. Samples were taken from two age groups; early-onset (55 years). Chi-square tests with odds ratio were used for data analysis. Results: The mean age of the early-onset group was 36±4.2 years, while the late-onset group was 62±6.9 years. AA genotype and A allele of P21 and TT genotypes and T allele of FGFR2 were significantly more frequent and were associated with an increased risk of early-onset of breast cancer (95%CI: 2.54 and 1.59; 2.63 and 1.64, respectively). Conclusions: Our study indicates that the A allele of P21 and the allele T of FGFR2 may be associated with an increased risk of early-onset of breast cancer in Yogyakarta, Indonesia. Further analysis is needed to confirm the findings.
- انتشار مقاله: 31-04-1397
- نویسندگان: Dewajani Purnomosari,Clarista Raharjo,Alvin Santoso Kalim,Rahma Herviastuti,Markus Yushan,Rangga Athilah Fajar,Karina Kazia Harris,Artanto Wahyono
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: molecular subtypes,Breast carcinoma,histological grade,lymph node metastases
- چکیده:
- چکیده انگلیسی: Objective: Breast carcinoma is a heterogeneous disease which is rich in diversity. Molecular subtypes of breast
cancer, histological grade and lymph node metastases are strong prognostic and predictive factors. In Indonesia,
only a limited number of studies have investigated the correlation between molecular subtypes with histological
grade and lymph node metastases. Methods: We analyzed 247 invasive breast carcinoma cases from the Anatomic
Pathology Installation of Dr. Sardjito General Hospital Yogyakarta between 2012-2015. The slides were stained for
estrogen receptors (ER), progesterone receptors (PR), HER2, Ki-67 and CK5/6 for classification into breast cancer
subtypes (BCS). Histological grade using the Nottingham system and lymph node status were obtained from anatomic
pathology records. The association between histological grade and lymph node status with BCS was examined with
Chi-square tests. Results: The immunohistochemical features of 247 cases of women with invasive breast carcinoma
were examined. There were 102 (41.3%) patients with Luminal A, 34 (13.8%) patients with Luminal B, 48 (19.4%)
patients with HER2-positive, and 63 (25.5%) patients with triple negative breast cancer (TNBC). There were 148
(59.9%) patients with negative lymph node status and 99 (40.1%) with positive status. Among 63 TNBC cases, 37
(58.7%) patients were positive for CK5/6 staining (basal-like). Statistically, there were significant differences between
histological grade and subtypes (p=0.013). However, no significant differences were found for lymph node metastases
(p=0.540). Conclusion: Among subtypes, Luminal A has the highest frequency, followed by TNBC, HER2-positive
and Luminal B. Histological grade was associated with molecular subtypes of breast carcinoma in Yogyakarta. Grade
I was associated with Luminal A, while Grade III was associated with Luminal B, HER2 and TNBC subtypes.- انتشار مقاله: 18-06-1396
- نویسندگان: Yunita Setyawati,Yeni Rahmawati,Irianiwati Widodo,Ahmad Ghozali,Dewajani Purnomosari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,Canna edulis,Adenomatous Polyposis Coli,Inducible Nitric Oxide Synthase
- چکیده:
- چکیده انگلیسی: Objective: Dietary high fibre and calcium intake has been suggested to reduce colorectal cancer risk. However, there
is limited information available regarding the potential of edible canna (Ganyong), with high dietary fibre and calcium
content, to act as a preventive agent for colorectal cancer. This experimental study was conducted to investigate the
preventive effect of Ganyong in reducing colorectal carcinogenesis with attention to effects on adenomatous polyposis
coli (APC) and inducible nitric oxide synthase (iNOS) expression. Methods: Thirty male Wistar rats were divided
into 5 equal groups; a normal control group without azoxymethane/dextran sodium sulphate (AOM/DSS) induction
and Ganyong, a ‘cancer’ control group with AOM/DSS induction only, and three treatment groups with AOM/DSS
induction and different percentages (5%, 10% and 20%) of Ganyong. Paraffin-embedded sections of rat colon tissue
were analysed by haematoxylin-eosin and immunohistochemical staining against antibodies against APC and iNOS.
Variation in rates of APC and iNOS expression were analyzed using the Kruskal-Wallis test followed by the Dunn’s
test (SPSS statistic version 24). Pthe expression of APC (p=0.013) and iNOS (p=0.013) compared to the normal control group. APC expression in the
treated groups was lower than in the ‘cancer’ control group (p=0.049), especially in the 10% Ganyong group (p=0.02).
In contrast, there was no significant variation among the treated groups regarding iNOS expression. Histopathological
features of the colon supported the data for APC and iNOS expression. Conclusion: This study indicated potential
chemopreventive effects of Ganyong reducing expression of factors contributing to colorectal carcinogenesis.- انتشار مقاله: 18-08-1396
- نویسندگان: . Burhannudin,Nur Mahmudah,Sitarina Widyarini,Dewajani Purnomosari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: age,molecular subtypes,Breast carcinoma,tumor size
- چکیده:
- چکیده انگلیسی:
Objective: Breast carcinoma (BC) is a heterogeneous disease that exhibits variation in biological behaviour, prognosis and response to therapy. Molecular classification is generally into Luminal A, Luminal B, HER2+ and triple negative/basal-like, depending on receptor characteristics. Clinical factors that determined the BC prognosis are age and tumor size. Since information on molecular subtypes of Indonesian BCs is limited, the present study was conducted, with attention to subtypes in relation to age and tumor size. Methods: A retrospective cross-sectional study of 247 paraffin-embedded samples of invasive BC from Dr. Sardjito General Hospital Yogyakarta in the year 2012- 2015 was performed. Immunohistochemical staining using anti- ER, PR, HER2, Ki-67 and CK 5/6 antibodies was applied to classify molecular subtypes. Associations with age and tumor size were analyzed using the Chi Square Test. Results: The Luminal A was the most common subtype of Indonesian BC (41.3%), followed by triple negative (25.5%), HER2 (19.4%) and luminal B (13.8%). Among the triple negative lesions, the basal-like subtype was more frequent than the non basal-like (58.8 % vs 41.2%). Luminal B accounted for the highest percentage of younger age cases (< 40 years old) while HER2+ was most common in older age (> 50 years old) patients. Triple negative/basal-like were commonly large in size. Age (p = 0.080) and tumor size (p = 0.462) were not significantly associated with molecular subtypes of BC. Conclusion: The most common molecular subtype of Indonesian BC is luminal A, followed by triple-negative, HER2+ and luminal B. The majority of triple-negative lesions are basal-like. There are no association between age and tumor size with molecular subtypes of Indonesian BCs.- انتشار مقاله: 18-06-1396
- نویسندگان: Yeni Rahmawati,Yunita Setyawati,Irianiwati Widodo,Ahmad Ghozali,Dewajani Purnomosari
- مشاهده