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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HPLC,eugenol,MTT,Syzygium aromaticum,HeLa Cell Line
- چکیده:
- چکیده انگلیسی: Background: The intendment of this study is to determine the pursuance in – vitro anticancer activity and
cytotoxicity of Syzygium aromaticum against the human cervical cancer cell line (HeLa) compared to the normal cell
lines. Apoptogenic properties of DCM extract of Eugenol was determined in this entire study. Materials and Methods:
HeLa cell lines were cultured in DMEM medium and incubated with different concentration of DCM – Eugenol
extract. MTT assay brought out the way to determine the cell viability and quantification was done with the optical
absorbance at 570 nm and 620 nm as reference. Apoptotic cells were affirmed by dual staining using acridine orange
bromide. Besides, the morphology of the nucleus was also confirmed by dual staining. Eugenol inhibited 50% growth
(IC50) of HeLa cell lines at 200 mg/ml of extract concentration. Results: Inhibitory efficacy of eugenol isolated from
Syzyzgyium aromaticum showed the cell – viability in time and dose dependent manner with consistent morphological
changes. Flow cytometer determined the apoptosis confirming the cytotoxicity value for MTT at IC50 with 81.85% cell
viability. Dual staining firmly enacts the damaged cells due to AO indicating apoptosis confirmation by dual staining.
Morphological analysis also clearly states that nil apoptosis has been seen in control and similarly in eugenol treated
when compared to cancerous HeLa cell – line. Conclusion: Evaluation of cytotoxicity effect of eugenol isolated from
Syzygium aromaticum showed it can be unrivalled dormant source of prodigious changes in HeLa cell line indicating
(revealing) that chemotherapeutic agent.- انتشار مقاله: 19-11-1396
- نویسندگان: Arunava Das,Harshadha K,Dhinesh Kannan S K,Hari Raj K,Bindhu Jayaprakash
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Histopathology,Cisplatin,Cicer arietinum,Chemoprotective,Alpha Esterase
- چکیده:
- چکیده انگلیسی: Traditional plant medicines are used for a range of cancer conditions. The chickpea is highly proteinaceous and
consumed as a staple in many parts of the world. An evaluation of chemoprotective and immunomodulatory activities
of Cicer arietinum (CE) in cisplatin-induced immunosuppressed mice was here performed. Cisplatin was given
at 100mg/kg, intraperitoneally, and after induction of immunosuppression mice were treated with Cicer arietinum
(0.5 mg/dose/animal/IP) for a period of 10-day. The influence of the extract on lymphoid organ weight, bone marrow
cellularity, alpha esterase activity and on enzyme levels such as (SGOT,SGPT, Urea, Creatinine was assessed to
identify any chemoprotective influence. Administration of CE to cisplatin-treated mice alleviated the drastic reduction
in bone marrow cellularity and α- esterase positive cells seen with cisplatin. Thus myelosuppression was reversed or
inhibited. Cisplatin bids to DNA and causes damage resulting in chromosome breaks, micronucleus formation and cell
death. CE is comprised of numerous middle-chain aliphatic alcohols, aldehydes and ketones, in addition to compounds
like 7-hydroxy-1-methoxy-6-methylanthraquinone, cyclohexadecane (CAS) and 6-(amino methyl)-2-naphthol. These
latter are thought to contribute to the characteristic aroma of C. arietimnum. In conclusion, administration of CE
in cisplatin-treated mice, boosted bone marrow cellularity and increased α- esterase positive cells, thus reversing
myelosupproession.- انتشار مقاله: 20-07-1396
- نویسندگان: Bindhu Jayaprakash,Arunava Das
- مشاهده