در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rs3746444 Polymorphism miR,499 Colon Cancer
- چکیده:
- چکیده انگلیسی: Background: Single nucleotide polymorphisms (SNPs) have been introduced as a new genomic source for cancer. Therefore, it was decided to conduct a study to evaluate the rs3746444 polymorphism of miR-499 in patients with colon cancer in comparison with healthy subjects. Methods: This case-control study was conducted to investigate rs3746444 polymorphism of miR-499 in blood samples of case and control groups. Patients with a confirmed diagnosis of cancer based on pathologic report were enrolled in the study as the case group and compared with healthy subjects. The level of significance was considered at p< 0.05. Result: The mean of DNA count in samples was 63.17± 23.51 that was significantly higher in the case group. The rs3746444 polymorphism of miR-499 was significantly higher in patients with cancer compared to the healthy subjects (P < 0.05). Conclusion: In this study, rs3746444 polymorphism of miR-499 was significantly higher in patients with colon cancer, which indicated that people with this polymorphism had a higher risk for malignancy.
- انتشار مقاله: 16-07-1398
- نویسندگان: Behjat Kalantari Khandani,Sara Shafieipour,Amir Mobasserfar,Mohammad Javad Zahedi,Mohammad Hashemi,Mohammad Mahdi Hayatbakhsh Abbasi,Sodaif Darvish Moghadam
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Prognostic factors,Ascites,mast cell leukemia,systemic mastocytosis,multiple organ damage
- چکیده:
- چکیده انگلیسی: Mast Cell Leukemia (MCL), a rare subtype of systemic mastocytosis is defined by bone marrow involvement as atypical and aleukemic mast cells, if more than 20% and less than 10% of peripheral WBCs are mast cells, respectively.
We met a case of aleukemic MCL presenting with anemia and ascites for 2 years, referred for BM evaluation, suspicious of leukemia. Our findings included BM involvement by diffused aggregates of oval- and spindle-shaped atypical mast cells, lacking mature mast cells and other hematopoietic cells. The mast cells were absent in peripheral blood smear. Further assessments showed positive reaction of mast cells metachromatic granules with Tryptase, Giemsa and Toluidine blue stains, the expression of CD117/KIT and CD45 by immunohistochemistery, and elevated level of serum Tryptase.
Radiologic investigations revealed generalized lymphadenopathy, and massive hepatosplenomegaly, followed by the cervical lymphadenectomy, and liver wedge biopsy. Suspicious peritoneal lesions were identified and underwent excisional biopsy. Microscopic evaluations showed lymph nodes and liver involvement by cancer cells and the same features in peritoneal seeding. Multiple organs damage progressed in few months and the patient died despite surgery and chemotherapy.
In conclusion, we report an extremely rarecase of aleukemic MCL with multiple organs damage such as liver, peritoneum, spleen, gastrointestinal tract and BM, presenting by ascites. According to this case and previous parallel studies, we suggest some clinicopathologic features in favor of poor prognosis, including the presence of multiple organs damage, hepatomegaly, ascites, peritoneal seeding, the absence of mature mast cells and other hematopoietic cells in the BM, and elevated serum Tryptase level.- انتشار مقاله: 07-10-1397
- نویسندگان: Elham Jafari,Ali Hadipour,Behjat Kalantari Khandani,Firoozeh Abolhasani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Prognosis,neoadjuvant therapy,Ki 67
- چکیده:
- چکیده انگلیسی: Background and objective:Breast cancer is the most common malignancy among women. The Neoadjuvant chemotherapy is the treatment of choice for non-operable tumors. The Ki67 is a proliferation marker that can be used to predict the therapeutic response to chemotherapy and the patients' prognosis.
Methods: This retrospective study was carried out on 55 consecutive patients with breast cancer referred to a Training Tertiary Healthcare Center in Kerman, Iran since 2009 to 2014. After diagnostic approval, the tissue samples of patients were examined for estrogen and progesterone receptors, ki67 and HER2-neu markers by using immunohistochemical staining. Then the patients were treated with 6 cycles of Neoadjuvant chemotherapy regimens by Doxorubicin and Taxans or 4 chemotherapy cycles, containing Anthracycline and Cyclophosphamide and 4 cycles of Paclitaxel. After mastectomy, their samples were reexamined for ki67 again and classified into three groups (low: ki67<15%), medium (Ki67 = 16-30%) and high (Ki67> 30%).
Results: Before chemotherapy, 54.5% of the patients had high expression of Ki67. But after chemotherapy, 52.7 of the patients had complete therapeutic response showing that the Ki67 level was reduced significantly (P=0.003).
Conclusion: Before and after Neoadjuvant chemotherapy, Ki67 measurements may be used as a predictive marker of therapeutic response.- انتشار مقاله: 29-06-1395
- نویسندگان: Vahid Moazed,Elham Jafari,Behjat Kalantari Khandani,Ali Nemati,Seyedamir Benrazavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial,Antioxidant,Anticancer,Acantholimon austroiranicum,Acantholimon serotinum,Acantholimon chlorostegium
- چکیده:
- چکیده انگلیسی: Objective: Acantholimon is a genus of perennial plant within the Plumbaginaceae family. Here, we aimed to investigate anticancer, antioxidant, and antibacterial potential of methanol extract of three Iranian endemic species of Acantholimon including A. austro-iranicum, A. serotinum and A. chlorostegium.
Materials and Methods: MTT assay was used to evaluate the in vitro cytotoxicity and apoptosis induction was examined by annexin V-PE apoptosis detection kit. Antioxidant activity was reported based on the DPPH-scavenging and DCF-DA assay. Antibacterial activity was measured by disc diffusion and micro-well dilution assay.
Results: MTT assay showed less cytotoxicity of methanol extracts against the HUVEC normal cell line (IC50 values: 817-900 µg/ml) compared to cancer cell lines MCF-7, HT29, SH-SY5Y, NCCIT and A549 (IC50 values: 213 to 600 µg/ml) that show the specificity of extracts toward cancer cells. Plant extract showed apoptosis induction and cell cycle arrest at the G0/G1 phases documented by annexin V staining and flow cytometry. According to antioxidant tests, extracts exhibited significant DPPH scavenging potential (IC50 values: 30-37 µg/ml) and could protect against H2O2-induced oxidative stress. Antibacterial activities showed a stronger inhibitory effect on Escherichia coli and Pseudomonas aeruginosa as Gram- negative bacteria (diameter of inhibition zone: 11-13 mm and minimal inhibition concentration (MIC): 3.175 to 12.5 mg/ml) compared to Gram-positive bacteria including Enterococcus faecalis and Staphylococcus aureus (diameter of inhibition zone: 3-7 mm and MIC: 25 to 50 mg/ml).
Conclusion: Our results suggested moderate cytotoxic and antibacterial potential and noteworthy antioxidant activity for the examined Acantholimon species.- انتشار مقاله: 06-11-1398
- نویسندگان: Sara Soltanian,Mahboubeh Sheikhbahaei,Mansour Mirtadzadini,Behjat Kalantari Khandani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Metastasis,Breast Neoplasm,Kerman
- چکیده:
- چکیده انگلیسی:
Background: Metastasis is important in survival and the quality of life of female breast cancer patients. This study was run in order to investigate metastasis and its related factors in female breast cancer patients in Kerman province from 2005 to 2015. The aim of this study was to investigate the factors associated with secondary metastasis in female breast cancer patients in the largest province of Iran (Kerman). Materials and Methods: In this case-control study, medical records of women diagnosed with breast cancer between the years of 2005 to 2015 were studied. Among them, 200 breast cancer patients with secondary metastasis were randomly selected as cases and 400 patients without metastasis were selected as the control group. Backward logistic regression, chi-square test, t-test and Mann-Whitney-U test were performed for comparing the two groups in SPSS 22. Results: Disease stage at diagnosis was significantly associated with secondary metastasis (p<0.001). Female patients with stage III breast cancer at the time of diagnosis had a higher chance OR=9.11 (CI 95%=2.99-27.76) of metastasis than women with stage 0 and 1 at diagnosis. Also, patients from rural areas had a higher chance for metastasis OR=1.89 (CI 95%=1.04-3.41). Other factors such as age, tumor size, tumor grade at diagnosis, hormone receptor status of estrogen and progesterone and Her-2 showed no significant association with metastasis (p>0.05). Conclusion: Stage at diagnosis and the residential location of patients were the most important factors related to secondary metastasis. These results emphasize the importance of early diagnosis, adequate education and awareness for breast cancer screening.- انتشار مقاله: 22-12-1395
- نویسندگان: Behjat Kalantari Khandani,Leila Tavakkoli,Narges Khanjani
- مشاهده