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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Toxoplasma gondii,Anterior uveitis,Kitten
- چکیده:
- چکیده انگلیسی: Ocular lesions are a common manifestation of toxoplasmosis in cats. In this survey, 10 healthy Syrian
mice were infected through intraperitoneal inoculation of bradyzoites of Toxoplasma gondii (Tehran strain).
Mice were killed 30 days after inoculation and the suspension of their brains was prepared, after confirmation
of the infection. Seven kittens were fed with the infected mice brain suspension. The kittens were 2.5–3
months old and had body weights of 650–900 g. All kittens shed oocyst 7–8 days after infection, and had
antibody titers as high as 1/320–1/1280 30 days after the challenge. Serum chemistry and complete blood
count were determined in all of the kittens. Examination of eyes was done with ophthalmoscope twice during
one week. Only one kitten showed unilateral anterior uveitis on day 16 after the infection was diagnosed
based on serology, oocyst shedding and other clinical signs.- انتشار مقاله: 22-03-1384
- نویسندگان: B. Mosallanejad,A. Malmasi,M. Mohebali,M. Tabatabayi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Chlamydia felis,Chlamydiosis,PCR,Ahvaz,Cat
- چکیده:
- چکیده انگلیسی: Chlamydiae are obligate generally Gram-negative intracellular parasites with bacterial characteristics, including a cell wall, DNA, and RNA. They have a worldwide distribution in different animal species. Chlamydia felis (C. felis) is an important agent with zoonotic susceptibility often isolated from cats with chronic conjunctivitis. The aim of the present survey aimed to determine the molecular occurrence of C. felis in cats in Ahvaz, Iran. In this regard, a total of 152 cats (126 households and 26 feral) were included in the current study. After recording their history information, two swabs were taken from the oropharyngeal cavity and eye conjunctiva of the investigated cats. The extraction of DNA was followed by PCR targeting the pmp gene of C. Felis. In the next step, the positive samples were sequenced based on the Gene Bank. Out of 152 samples, 35 (23.03%) were positive using polymerase chain reaction technique (95% CI: 16.30-29.70). Regarding infection with Chlamydiosis, the obtained results showed a significant difference between cats suffering from ocular or respiratory diseases (44.64%; 25 out of 56) and the healthy ones (10.42%; 10 out of 96; P=0.01). The prevalence of infection was significantly higher in cats younger than 1 year (34.12%; 29 out of 85), compared to those older than 1 year (8.96%; 6 out of 67; P=0.02). No significant difference was noted in terms of gender (25.45% in males and 21.65% in females), breed (23.81% in DSH and 19.23% in Persian), and lifestyle (22.22% companions [28 out of 126] and 26.92% ferals [7 out of 26]; P>0.05). It can be concluded that a significant number of cats are infected with C. felis in Ahvaz. The use of molecular tests, such as PCR, has revolutionized the diagnosis of chlamydial infections.
- انتشار مقاله: 06-10-1396
- نویسندگان: M. Barimani,B. Mosallanejad,M. Ghorbanpoor,S. Esmaeilzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Ahvaz,Dog,Neospora caninum,Neospora agglutination test (NAT),Seroprevalence
- چکیده:
- چکیده انگلیسی: Dogs are important in the epidemiology of Neospora caninum because they act as definitive hosts, shedding oocysts in the environment. The aim of the present survey was to evaluate the serological prevalence of Neospora caninum infection in urban and rural dogs in Ahvaz district, southwest of Iran. In this study, blood samples were taken from 100 rural dogs and 50 urban dogs. The dogs were categorized into two age groups (i.e., ≤ 3 and > 3 years). Neospora agglutination test (NAT) was performed for the detection of infection. Among 150 samples, 30 (20%) showed infection in 1:50 to 1:800 dilutions by NAT (confidence interval 95%: 13.60-26.40). The antibody titers were as follows: 1:50 (n=1), 1:100 (n=14), 1:200 (n=3), 1:400 (n=10) and 1:800 (n=2). The highest serum dilution was 1:100 in 46.67% of the infected dogs and the lowest serum dilution was 1:50 in 3.33% of them. The obtained results showed a significant difference in seroprevalence between urban (10%) and rural (25%) dogs (P=0.03). Although the seroprevalence was higher in dogs above three years of age (23.33%) than below three years (17.78%), there was not a significant difference among different age groups in this regard (P>0.05). The possibility of infection in dogs above the age of three years was 1.3 more than those below three years of age (confidence interval 95%: 0.58-2.9). It can be concluded that a relatively considerable percentage of dogs in Ahvaz district are infected with N. caninum. These infected dogs can play an important role in the transmission of neosporosis to other animals.
- انتشار مقاله: 12-06-1395
- نویسندگان: B. Mosallanejad,S. Bahrami,H. Hamidinejat,S. Ghanavati
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Chlamydia felis,Chlamydiosis,PCR,Ahvaz,Cat
- چکیده:
- چکیده انگلیسی: Chlamydiae are obligate generally Gram-negative intracellular parasites with bacterial characteristics, including a cell wall, DNA, and RNA. They have a worldwide distribution in different animal species. Chlamydia felis (C. felis) is an important agent with zoonotic susceptibility often isolated from cats with chronic conjunctivitis. The aim of the present survey aimed to determine the molecular occurrence of C. felis in cats in Ahvaz, Iran. In this regard, a total of 152 cats (126 households and 26 feral) were included in the current study. After recording their history information, two swabs were taken from the oropharyngeal cavity and eye conjunctiva of the investigated cats. The extraction of DNA was followed by PCR targeting the pmp gene of C. Felis. In the next step, the positive samples were sequenced based on the Gene Bank. Out of 152 samples, 35 (23.03%) were positive using polymerase chain reaction technique (95% CI: 16.30-29.70). Regarding infection with Chlamydiosis, the obtained results showed a significant difference between cats suffering from ocular or respiratory diseases (44.64%; 25 out of 56) and the healthy ones (10.42%; 10 out of 96; P=0.01). The prevalence of infection was significantly higher in cats younger than 1 year (34.12%; 29 out of 85), compared to those older than 1 year (8.96%; 6 out of 67; P=0.02). No significant difference was noted in terms of gender (25.45% in males and 21.65% in females), breed (23.81% in DSH and 19.23% in Persian), and lifestyle (22.22% companions [28 out of 126] and 26.92% ferals [7 out of 26]; P>0.05). It can be concluded that a significant number of cats are infected with C. felis in Ahvaz. The use of molecular tests, such as PCR, has revolutionized the diagnosis of chlamydial infections.
- انتشار مقاله: 06-10-1396
- نویسندگان: M. Barimani,B. Mosallanejad,M. Ghorbanpoor,S. Esmaeilzadeh
- مشاهده