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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: It has been reported that several species of Helicobacter colonize the biliary tract of animals and human, but their participation in hepatobiliary diseases are not established. This study is undertaken to determine if Helicobacter genus members, especially with regard to H. pylori, could be detected in the bile and gallbladder diseases. Methods: Thirty-eight gallbladder tissues and thirty-six bile samples recovered from 38 patients with different gallbladder diseases were subjected to rapid urease test, culture and an established Multiplex-PCR using two pairs of primers based on 16s rRNA and isocitrate dehydrogenase genes specific for Helicobacter genus and H. pylori species, respectively. Bile and tissue samples from 40 autopsy gallbladders with normal pathology were tested by PCR as control group. Results: In 4 of 36 bile samples H. pylori was identified using PCR. None of the tissue samples of the patients and controls were positive in PCR for Helicobacter DNA. We were not successful in isolation of Helicobacter using culture method. Conclusion: This study showed the presence of H. pylori DNA in the bile samples of a small sample size of patients with biliary diseases in south of Iran. We could find no pathogenetic role for H. pylori in the formation of hepatobiliary disease. To establish a clinical role for Helicobacter species in the hepatobiliary diseases, more studies on a larger group of patients and control groups are needed to ascertain whether Helicobacter species or their component might have a role as a causative agent or cofactor in the pathogenesis of biliary tract diseases in human.Iran J Med Sci 2006; 31(4): 186-190. Keywords ● H. pylori ● biliary diseases ● Cholecystitis ● human
- انتشار مقاله: 09-06-1394
- نویسندگان: S. Farshad,A. Alborzi,S.A. Malekhosseini,B. Gramizadeh,B. Oboodi,M. Rasouli,A. Japoni,M. Kalani,B. Pourabbas
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background/objective: Despite the present routine treatment of septic arthritis with antibacterial agents, articular damage is persistent and frequently leads to loss of joint function. The aim of this study was to assess the effect of intra-articular corticosteroids added to systemic antibiotics in the treatment of experimental staphylococcal knee joint infection in rabbits. Methods: Thirty rabbits were injected in their knees by Staphylococcus aureus. The rabbits were divided into 3 equal groups. In group A, rabbits received no treatment. In group B, rabbits were treated with systemic antibiotics alone. Group C, received systemic antibiotics and intra-articular corticosteroids. After 16 days animals were killed and knee joint X-Ray as well as histopathological–histochemical parameters were assessed. Results: All rabbits survived the experiment; the treated groups (B,C) had better histological–histochemical scores in comparison with the untreated group (A). Group C had significantly better scores in joint sections in comparison with group B (mean SD = 6.7 ± 2.3 v 4.0 ± 2.4; P= 0.019). Lower damage in the former group was expressed in lesser clustering of chondrocytes, proteoglycan depletion, and severity of synovitis. Radiological soft tissue scoring was significantly better in group C in comparison with group B. Three peri-articular abcesses were observed in group C but none in group B. Conclusion: Addition of intra-articularly administered corticosteroids to antibiotic treatment of septic arthritis improved histological histochemical parameters in this experimental setting, although on account of the clinical observation of three cases with peri-articular abcesses in this group, caution is warranted in interpretation of these results.
- انتشار مقاله: 06-08-1394
- نویسندگان: FM. Jaberi,M. Nicfar,N. Tanideh,B. Gramizadeh
- مشاهده