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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mutation,gallbladder cancer,tumor suppressor gene,proto-oncogene,Peru
- چکیده:
- چکیده انگلیسی: Background: Recent studies have shown that genetic alterations are associated with the effect of patient geographic
location on gallbladder cancer development. Peru has a high incidence of gallbladder cancer, but causative factors
have not yet been identified. We examined the frequency of mutations in TP53 and K-ras genes in Peruvian patients
with gallbladder cancer, and compared this with data from Bolivia, Hungary, Chile, and Japan, which have a high
gallbladder cancer incidence. Methods: DNA was extracted from formalin-fixed paraffin-embedded gallbladder tissue
sections of 30 gallbladder cancer patients (9 men and 21 women) obtained using microdissection. Mutations in exons
5 to 8 of TP53 and codons 12, 13, and 61 of K-ras were examined using direct sequencing. Results: TP53 mutations
were observed in 10 (33.3%) of patients, but K-ras mutations were absent. Nine (90%) TP53 mutations were point
mutations (7 missense and 2 silent mutations), and the most frequent substitution was a G:C to A:T transition. G:C to
A:T transitions at the CpG site or G:C to T:A transversions were found in one patient each. No significant differences
were found in the frequency of TP53 and K-ras mutations among patients in the 5 countries. Conclusions: Our findings
suggest that endogenous mechanisms and exogenous carcinogens may affect the carcinogenic process in Peruvian
gallbladder cancer patients, similar to that in Bolivian patients. Further studies with a larger sample size are needed
to clarify these findings.- انتشار مقاله: 02-07-1397
- نویسندگان: Tatiana Vidaurre,Sandro Casavilca,Paola Montenegro,Henry Gomez,Mónica Calderón,Jeannie Navarro,Jessica Aramburu,Ebert Poquioma,Yasuo Tsuchiya,Takao Asai,Yoichi Ajioka,Ayako Sato,Toshikazu Ikoma,Kazutoshi Nakamura
- مشاهده