در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Formaldehyde,Body Fluids,Nucleic Acids,Pre-Analytical Phase
- چکیده:
- چکیده انگلیسی: Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples.
- انتشار مقاله: 20-01-1399
- نویسندگان: Maryam Sotoudeh Anvari,Atoosa Gharib,Maryam Abolhasani,Aileen Azari-Yaam,Farzaneh Hossieni Gharalari,Moeinadin Safavi,Ali Zare-Mirzaie,Mohammad Vasei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Pseudomonas aeruginosa,IMP,VIM,Metallo-β-Lactamase
- چکیده:
- چکیده انگلیسی: Background & objective: Beta-lactam antibiotics resistance specifically Imipenem and Meropenem, the last choices of treatment, causes fatal events in patients with P.aeruginosa infection. The aim of this study was to detect the VIM and IMP of metallo-beta-lactamase genes in 103 isolates of P. aeruginosa in two Iranian hospitals.
Methods: In this study, we evaluated the susceptibility of P. aeruginosa to a range of β-lactam antibiotics using disk diffusion method as a standard biochemical test. Combined disk test of Imipenem (IMP) and Imipenem plus Ethylenediaminetetraacetic acid (EDTA) was performed as a phenotypic method to find metallo-beta-lactamase producing isolates.Using conventional PCR method; we evaluated VIM and IMP of metallo-beta-lactamase (MBL) genes in 103 isolates of P.aeruginosa.
Results: Twenty six (25.2%) out of 103 isolates were resistant to Imipenem and 26 (25.2%) to Meropenem. Among 26 Imipenem and Meropenem-resistant strains (25.2%), 19 cases (73.0%) were MBL producing. Using PCR method, we detected the blaVIM and blaIMP genes in 6 (5.8%) and 2(1.9%) of 19 MBL producing isolates, respectively.
Conclusions: Evaluation of these carbepenemases genes improve epidemiologic researches and also, can be used as a diagnostic tool for discriminating between antibiotics resistant and sensitive strains of P.aeruginosa as well as follow-up the patients after treatment.- انتشار مقاله: 29-09-1394
- نویسندگان: Behrang Kazeminezhad,Arezoo Bostanmanesh Rad,Atoosa Gharib,Sara Zahedifard
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Aspergillosis,Gastrointestinal tract
- چکیده:
- چکیده انگلیسی: Gastrointestinal aspergillosis most often occurs in the setting of disseminated infection from a primary pulmonary site and primary gastrointestinal aspergillosis is an unusual presentation. We report a 7 year old female child with aplastic anemia, who was under treatment but experienced periods of neutropenia. She had no evidence of respiratory aspergillosis before the onset of abdominal symptoms including severe abdominal pain and rectorrhagia.The patient died of septic shock two weeks after emergency surgery for rectal bleeding. Although a rare condition, primary gastrointestinal aspergillosis should be considered in differential diagnosis of gastrointestinal symptoms in neutropenic patients.
- انتشار مقاله: 07-07-1393
- نویسندگان: Atoosa Gharib,Azadeh Rakhshan,Farzaneh Jadali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Sexual dysfunction,Autologous,platelet,growth factor,pelvic irradiation
- چکیده:
- چکیده انگلیسی: Sexual dysfunction (SDF) is a common sequel to cancer treatment which affects the quality of life in women
treated with pelvic radiotherapy. The aim of this study was to evaluate the safety, symptom resolution and objective
improvement the injection of autologous platelet released growth factor (APRGF) for treatment of SDF in cited patients.
This prospective pilot study enrolled 10 cancer-free patients with SDF who underwent pelvic radiotherapy at least 5
years ago, randomly. Each patient was received 1-2 cc APRGF within four weeks and all patients were re-evaluated
at eight weeks and six months. CD34 immuno histochemistry and Masson’s trichrome staining were performed on
vaginal biopsy section for angiogenesis and fibrosis assay respectively. Sexual satisfaction after the injection of APRFG
was clinically difference and the entire patient had sexual satisfaction. In the patient’s follow-up, none of them needs
to repeat the treatment. Our results declared that APRGF injection was effective and symptoms were disappeared in
the entire patients. Significant objective improvements in vaginal diameter (mean before injection, 6.5 cm vs 7.1 cm
after injection) (p-value = 0.001) and vaginal flexibility (mean before treatment, 0.72 cm vs 1.85 cm after injection)
(P-value = 0.026) were observed. Characteristics of discharge before the injection in 60% of patients were included
dry vagina and 40% had mild discharge but after injection 40% of patients had moderate and also 60% had mild and
sufficient discharge (P-value= 0.190). Overally, our patients reported better sexual function and showed better vaginal
function indexes, after APRFG injection.- انتشار مقاله: 19-05-1397
- نویسندگان: Atefeh Samaie Nouroozi,Ashraf Alyasin,Ashraf Malek Mohammadi,Nili Mehrdad,Seyed Asadollah Mousavi,Mohammad Vaezi,Atoosa Gharib,Ardeshir Ghavamzadeh,Saeed Mohammadi
- مشاهده