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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Biological activity,cultivation,Essential oil composition,Thymus fedtschenkoi
- چکیده:
- چکیده انگلیسی: Backgruon and aims: The aim of this research was to compare the chemical composition and the antioxidant and antibacterial properties of wild and cultivated types of T. fedtschenkoi collected from East Azarbayjan, Iran.
Material and Methods: The essential oils (EOs) from aerial parts of wild and cultivated T. fedtschenkoi were investigated by gas chromatography/mass spectrometry (GC/MS). Antibacterial activities of EOs and methanol extracts were tested against bacteria by disc diffusion method and determining their minimum inhibitory concentration (MIC) values by agar dilution method and antioxidant activity by DPPH and FRAP assays.
Results: Twenty-two components were identified representing more than 99.20% of the total oil constituents. The major components in the oils of the wild type were; thymol (49.21%), p-cymene (15.20%) and Carvacrol (10.20%) and for the cultivated type were; thymol (62.33%) and carvacrol (7.12%). The MIC values of bacterial strains, which were sensitive to the EO of T. fedtschenkoi, were in the range of 2-128 μg/mL in wild type and 2-16 μg/mL in cultivated type.
Conclusion: Our data show that cultivation significantly affects the EOs’ chemical composition and antioxidant potential of T. fedtschenkoi. They signify a reasonable source of natural antibacterial substances that proved to be potential as a drug for use in pathogenic bacteria.The antibacterial activity by the presence of inhibition zone appeared from EOs in the cultivated type on all tested microorganisms significantly higher than that in the wild type, which can be attributed to the presence of high concentration of thymol (47.48%) against the wild type (29.96%).- انتشار مقاله: 04-07-1397
- نویسندگان: Asad Kazemi,Faranak Elmi,Alireza Moradi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Biological activity,cultivation,Essential oil composition,Thymus fedtschenkoi
- چکیده:
- چکیده انگلیسی:
Background and aims: The aim of this study was to characterize the chemical composition and antimicrobial properties of Achillea biebersteinii essential oil (EO).
Methods: The chemical composition of samples obtained from Marand city in East Azerbaijan, was assessed using gas chromatography mass spectrometry (GC/MS). The antimicrobial properties were evaluated by the disc diffusion method against methicillin-resistant Staphylococcus aureus (MRSA), other extended-spectrum beta-lactamases (ESBLs) producing, as well as Gram-negative and Gram-positive bacteria. The minimum inhibitory concentration (MIC) value of EO was assessed using the agar dilution method.
Results: In A. biebersteinii the major compounds were α-terpinene (41.42%), 2-carene (13.96%), m-cymene (13.41%) and 1,8-cineole(8.91%).The EO showed antimicrobial activity against ten microorganisms, especially Streptococcus sanguis, S. aureus (MRSA strain), and Klebsiella pneumoniae (ESBL-producing strain), which was potentially better than tetracycline and kanamycin.
Conclusion: This study confirmed that EO of A. biebersteinii has in vitro antimicrobial activity against Gram-negative and Gram-positive bacteria, which has made it an alternative antibacterial agent.- انتشار مقاله: 15-11-1397
- نویسندگان: Asad Kazemi,Faranak Elmi
- مشاهده