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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: RT-PCR,Survival,imatinib,BCR-ABL,Chronic myeloid leukemia
- چکیده:
- چکیده انگلیسی: Background: The prognostic significance of the common BCR-ABL transcripts like e13a2 (b2a2) and e14a2
(b3a2) in Chronic myeloid leukemia (CML) has been reported from patients treated with different tyrosine kinase
inhibitors but its impact on clinical response and overall survival remains still unexplored. The aim of this study was
to evaluate the prognostic significance of different transcript types in a cohort of CML patients treated with imatinib.
Methods: A total 42 confirmed cases of Chronic Myeloid Leukemia (CML) patients were recruited into our cohort
study and a multiplex Reverse Transcriptase-Polymerase Chain Reaction technique (RT-PCR) was used to detect 3 main
transcript types ‘e1a2’, ‘e13a2’, and ‘e14a2’ found in CML. Results: Only two types of transcripts e13a2 (b2a2) and
e14a2 (b3a2) were detected in our CML patients and none had the e1a2 type. All the patients were RT-PCR positive
for either e13a2 or e14a2 fusion transcript demonstrating 100% concordance with their Ph+ve cytogenetic status at
baseline. TLC count (range of 201-600x103/μl) and platelet count (range of 201-900x103/μl) at baseline were found to
be associated more with the e14a2 (b3a2) than the e13a2 (b2a2) transcript type (p-value: 0.001). The two transcripts
found did not relate significantly towards sex, age-group or indicated spleen size ranges as well as percentage ranges
of blast cells. Conclusion: We conclude that there is no overall prognostic implication of either the e13a2 or the e14a2
transcript type across the spectrum of indicated clinical parameters evaluated. Even the overall survival analysis of the
two transcript types revealed no prognostic association whatsoever.- انتشار مقاله: 13-03-1396
- نویسندگان: Niyaz A Azad,Zafar A Shah,Arshad A Pandith,Mosin S Khan,Roohi Rasool,Javed Rasool,Shiekh A Aziz
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,hypermethylation,UNC5C,Netrin-1,DCC
- چکیده:
- چکیده انگلیسی: Background:The development of Colorectal Cancer (CRC) is a complex multistep process involving an accumulation
of multiple genetic and epigenetic alterations. Epigenetic modifications, particularly DNA methylation in selected
gene are recognized as common molecular alterations in human tumors. Netrin-1 receptors are aberrantly methylated
in primary colorectal cancer. Epigenetic alterations in the netrin-1 receptors have been found to be related with the
malignant potential of CRC. Purpose: In the present study, we evaluated the role of promoter hypermethylation
of UNC5C gene (one of the netrin-1 receptors) in colorectal cancer patients of Kashmiri population (North India).
Hypermethylation in tumour tissue was detected by Methylation- Specific Polymerase Chain Reaction (MS-PCR).
Results: UNC5C promoter hypermethylation was significantly found to be associated with colorectal cancer cases
where frequency was 62% (31 of 50) and 38% (19 of 50) patients were unmethylated (p<0.0001).UNC5C methylation
was significantly higher in CRCs with a frequency of 62% than 10% in corresponding normal mucosa of (p<0.0001).
Further, UNC5C hypermethylation was found to be significantly associated with stage-III/IV as compared to stage I/II
with a frequency of 75.8% and 42.8% respectively(p>0.05). Conclusion: We conclude that UNC5C hypermethylation
is implicated in CRC which plays a role in its tumorigenesis and may predict the late stage disease.- انتشار مقاله: 15-11-1395
- نویسندگان: Sartaj A Guroo,Ajaz A Malik,Dil Afroze,Shazia Ali,Arshad A Pandith,Adfar Yusuf
- مشاهده