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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Opium,Spermatogenesis Indexes,Testis Histology
- چکیده:
- چکیده انگلیسی: Background: Opium, as the most widely known opioid in the world, has significant effects on reproductive organs, which can affect fertility and influence the next generation. This study was performed to investigate the effects of opium administration on histological changes and spermatogenesis indexes in the testis of adult male rats. Methods:A total of 50 adult male Wistar rats were used. Animals were divided into 5 groups (n=10). Control (intact), Vehicle (received saline for 7 and 14 days) and Opium (received 2 mg/kg opium i.p. For 7 and 14 days) groups. Sample of testes were stained by H&E, then the number of spermatogonia, spermatocytes and Leydig cells were counted and spermatogenesis indexes which included tubular differentiation index (TDI), spermatogenesis index (SI), repopulation index (RI) and means of seminiferous tubules diameter (MSTD) were determined. Results: In opium groups, the number of seminiferous tubules cells and spermatogenesis decreased compared with the control group (P<0.001). Moreover, the results showed that TDI, SI, RI and STD parameters significantly decreased in 7th and 14th days, compared with the control group (P<0.001). Conclusion: The data showed the adverse effects of opium administration (for 7 and 14 days) on spermatogenesis and its indexes in rat testis tissue. The underlying mechanisms need further investigation.
- انتشار مقاله: 03-09-1398
- نویسندگان: Fateme Hasani,Zohreh Safi,Behzad Razavi,Kouros Divsalar,Arezoo Saberi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Folic acid,Cotrimoxazole,Ovarian maturation status,Ovarian histopathology
- چکیده:
- چکیده انگلیسی: Background: Previous studies have reported the antifertility activities of sulfonamides. This study was designed to evaluate the effects of co-trimoxazole and its co-administration with folic acid on ovarian tissue in female rats.Methods: A total of 54 rats were randomly divided into 9 groups (n=6). Group I served as the control and group II (vehicle) received saline. Other groups, III to IX, received co-trimoxazole (30, 60, and 120 mg/kg; i.p.), folic acid (1 mg/kg; i.p.) or their combination for 14 days, respectively. The oocytes were obtained from each group at the end of the 14th days and scored for maturational status as germinal vesicle (GV), metaphase I (MI), or metaphase II (MII). The number of primordial follicle (PrF), primary follicle (PF), and secondary follicle in formalin-fixed ovaries were counted under light microscopy. The data were analyzed by one-way ANOVA followed by post-hoc Dunnet test using SPSS statistical software (version 17.0). Results were considered statistically significant at P<0.05.Results: Co-trimoxazole (60 and 120 mg/kg) treatment for 14 days caused a significant decrease in the number of GV (P=0.02, P<0.001), MI and MII (P=0.03, P<0.001), a significant increase in structural abnormalities, including PrF, PF and secondary follicle (P<0.001) as well as congestion, inflammation and necrosis of ovarian tissue compared to the vehicle group. Folic acid co-administration with co-trimoxazole reversed partially all these parameters compared to the co-trimoxazole group (P<0.001).Conclusion: The data showed the adverse effects of co-trimoxazole on the ovarian maturational status and tissue structure which was reversed partially by folic acid co-administration in rats.
- انتشار مقاله: 04-05-1395
- نویسندگان: Arezoo Saberi,Ehsan Salarkia,Zohreh Safi,Gholamreza Sepehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Methamphetamine,Rats,histology,spermatogenesis,Testis
- چکیده:
- چکیده انگلیسی: Background: Methamphetamine (mAMP) as a recreational drug has devastating effects on the central nervous system (CNS). Several studies have shown that mAMP has inhibitory effects on oogenesis and spermatogenesis, and causes impaired fertility. This study designed to investigate the effect of mAM Padministration on histological changes and spermatogenesis indices in the testis of adult male rats.Methods: In this experimental study, 50 male Wistar rats were randomly divided into control (received no treatment, n = 10), vehicle (received saline for 7 and 14 days, n = 20), and experimental group [received mAMP, 5 ml/kg, intraperitoneal (IP) for 7 and 14 days, n = 20]. Testicular tissue samples were stained by hematoxylin and eosin (H&E) technique. For histological study, we counted the number of spermatogonia, spermatocytes and Leydig cells. Spermatogenesis indices which include: tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI) and the mean seminiferous tubules diameter (MSTD) were studied. Data were analyzed by one-way ANOVA, using SPSS software. P < 0.05 was considered statistically significant.Findings: This study showed that mAMP caused a significant decrease in number of seminiferous tubules cells and spermatogenesis in treated group compared with the control group. Moreover, results showed a significant decrease in spermatogenesis indices including TDI, SI, RI, and MSTD in 14th day, compared to control group (P < 0.001).Conclusion: The data showed the adverse effects of mAMP administration (for 7 and 14 days) on testes structure and spermatogenesis indices in rat testis tissue. The underlying mechanism(s) needs further investigation.
- انتشار مقاله: 28-11-1396
- نویسندگان: Arezoo Saberi,Gholamreza Sepehri,Zohreh Safi,Behzad Razavi,Faranak Jahandari,Kouros Divsalar,Ehsan Salarkia
- مشاهده