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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Synovial sarcoma,fish,Liposarcoma,MDM2,CHOP,SYT
- چکیده:
- چکیده انگلیسی: Background & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrangements involving multiple chromosomes. Fluorescence in situ hybridization (FISH) can be used to identify these chromosomal translocations and amplifications, and sub classify STS precisely. The current study aimed at investigating the usefulness of FISH, as a diagnostic ancillary aid, to detect cytogenetic abnormalities such as MDM2 (murine double minute 2) amplification and CHOP(C/EBP homologous protein) rearrangement in liposarcoma, as well as SYT (synaptotagmin) rearrangement in synovial sarcoma.
Methods: The FISH technique was used to analyze 17 specimens of liposarcoma for MDM2 amplification and CHOP rearrangement, and 10 specimens of synovial sarcoma for SYT rearrangement. The subtypes of liposarcoma and synovial sarcomas were reclassified according to the FISH results and compared with those of the respective histological findings.
Results: According to the FISH results in 17 liposarcoma cases, well-differentiated liposarcoma(WDLPS), dedifferentiated liposarcoma (DDLPS), and myxoidliposarcoma (MLPS)subtypes were 41%, 53%, and 6%, respectively. In different subtypes of liposarcoma, a total of 30% mismatches were observed between pathologic and cytogenetic results. According to the histological findings from FISH analysis, SYT rearrangement was found only in three out of 10 (30%) synovial sarcomas.
Conclusion: The detection of cytogenetic abnormalities in patients with liposarcoma and synovial sarcoma by FISH technique provides an important objective tool to confirm sarcoma diagnosis and sub classification of specific sarcoma subtypes in such patients.- انتشار مقاله: 19-03-1395
- نویسندگان: Farhad Shahi,Razieh Alishahi,Hossein Pashaiefar,Isa Jahanzad,Naser Kamalian,Ardeshir Ghavamzadeh,Marjan Yaghmaie
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: immunohistochemistry,Epstein Barr Virus Infections,Diffuse Large-Cell Lymphoma,B Cell Lymphoma
- چکیده:
- چکیده انگلیسی: Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin’s lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients. Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin’s lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients. Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin’s lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients. Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin’s lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients. Background and Objectives: Epstein Barr Virus (EBV) is one of the members of herpesviridae family and a sub-category of Gamma herpes virinae. EBV, which normally has CR2 or CD21 receptors on B-lymphocytes, has mutagenic features for them. The virus plays an important role in causing some malignant cancers. About 30% of the cases with non-Hodgkin’s lymphoma are diffuse large B-cell lymphoma (DLBCL). In the present study, the incidence rate of EBV in DLBCL was evaluated. Materials and Methods: Immunohistochemistry (IHC) and PCR methods were used for studying the relationship between EBV and DLBCL. Paraffin blocks of 116 patients from Sina & Shariati hospitals, Tehran, Iran, with DLBCL diagnoses in 2005-2009 were collected. EBV-LMP in IHC and PCR virus genome in PCR were examined. Results: Findings of the PCR method showed that 28 cases of the total 116 patients with DLBCL were EBV positive (the frequency of EBV positivity was correspondingly 40% and 60% in females and males) and this shows a 25.8% EBV frequency in DLBCL. IHC findings showed that six cases were EBV positive. The compatibility of positive IHC and PCR responses was two cases and there are four conflicting cases. Conclusion: It seems that PCR is a more appropriate method for diagnosing EBV and IHC cannot solely prove the presence of EBV in DCBCL patients.
- انتشار مقاله: 28-06-1393
- نویسندگان: Farid Kosari,Naghmeh Amin Taheri,Alireza Sadeghipour,Kamran Alimoghaddam,Ardeshir Ghavamzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Genotype,HLA,KIR,Acute Leukemia
- چکیده:
- چکیده انگلیسی: Background: Interaction between killer cell immunoglobulin-like receptors (KIR) and human leukocyte antigen (HLA) class I molecules is important for regulation of natural killer (NK) cell function.
Objective: The aim of this study was to investigate the impact of compound KIR-HLA genotype on susceptibility to acute leukemia.
Methods: Cohorts of Iranian patients with acute myeloid leukemia (AML; n=40) and acute lymphoid leukemia (ALL; n=38) were genotyped for seventeen KIR genes and their three major HLA class I ligand groups (C1, C2, Bw4) by a combined polymerase chain reaction–sequence-specific primers (PCR-SSP) assay. The results were compared with those of 200 healthy control individuals.
Results: We found a significantly decreased frequency of KIR2DS3 in AML patients compared to control group (12.5% vs. 38%, odds ratio=0.23, p=0.0018). Also, the KIR3DS1 was less common in AML group than controls (27.5% vs. 44.5%, p=0.0465, not significant after correction). Other analyses including KIR genotypes, distribution and balance of inhibitory and activating KIR+HLA combinations, and co-inheritance of activating KIR genes with inhibitory KIR+HLA pairs were not significantly different between leukemia patients and the control group. However, in AML patients a trend toward less activating and more inhibitory KIR-HLA state was observed. Interestingly, this situation was not found in ALL patients and inhibition enhancement through increase of HLA ligands and inhibi-tory combinations was the main feature in this group.
Conclusion: Our findings may suggest a mechanism for escape of leukemic cells from NK cell immunity.- انتشار مقاله: 16-05-1395
- نویسندگان: Farhad Shahsavar,Nader Tajik,Kobra-Zinat Entezami,Masoomeh Fallah Radjabzadeh,Behnam Asadifar,Kamran Alimoghaddam,Mohammadreza Ostadali Dahaghi,Arash Jalali,Andisheh Ghashghaie,Ardeshir Ghavamzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Multiple Sclerosis,Mesenchymal Stem Cells
- چکیده:
- چکیده انگلیسی: Background: Mesenchymal stem cells (MSCs) with their potential to differentiate into mesodermal and non-mesodermal lineages have several immunomodulatory characteris-tics. These properties make them promising tools in cell and gene therapy.
Objective: To evaluate the potential therapeutic applications of autologous MSC in improving clinical manifestations of MS patients.
Methods: Ten patients were included in this pi-lot study. All had progressive disease that had not responded to disease modifying agents including Mitoxantrone. Their Expanded Disability Status Scale (EDSS) score ranged from 3.5 to 6. Patients were injected intrathecally with culture expanded MSCs. They were followed with monthly neurological assessment and a MRI scan at the end of the first year.
Results: During 13 to 26 months of follow up (mean: 19 months), the EDSS of one patient improved from 5 to 2.5 score. Four patients showed no change in EDSS. Five patients’ EDSS increased from 0.5 to 2.5. In the functional system assess-ment, six patients showed some degree of improvement in their sensory, pyramidal, and cerebellar functions. One showed no difference in clinical assessment and three deterio-rated. The result of MRI assessment after 12 months was as following: seven patients with no difference, two showed an extra plaque, and one patient showed decrease in the number of plaques.
Conclusion: This preliminary report emphasizes on the feasibility of autologous MSC for treatment of MS patients. However, in order to draw a definitive conclusion a larger sample size is required.- انتشار مقاله: 17-05-1395
- نویسندگان: Mandana Mohyeddin Bonab,Sepideh Yazdanbakhsh,Jamshid Lotfi,Kamran Alimoghaddom,Fatemeh Talebian,Farnaz Hooshmand,Ardeshir Ghavamzadeh,Behrouz Nikbin
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: HLA typing,Cord blood,PCR-SSOP,HLA Class I
- چکیده:
- چکیده انگلیسی: Background: HLA compatibility between transplant donor and recipient is one of the major determinants of transplant outcome.
Objective: To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors.
Methods: Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology, Oncology, and Bone Marrow Transplantation Research Center, was prepared and HLA class I was determined by the PCR-SSOP.
Results: A total of 284 HLA-A alleles was identified of which A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles, B*35, B*51, Cw*4 and Cw*12 were the most frequent alleles in the studied population.
Conclusion: Amplification of HLA loci with PCR-SSOP has proved to be a reliable method for HLA-A, -B and -C genotyping.- انتشار مقاله: 16-05-1395
- نویسندگان: Andisheh Ghashghaie Mansour,Seyyed Hamidollah Ghaffari,Kamran Ali-moghadam,Ardeshir Ghavamzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: CML,Cytokine Gene Polymorphism
- چکیده:
- چکیده انگلیسی: Background:It has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing Chronic Myelogenous Leukemia (CML). In this regard, Th1 and Th2 cytokines and their gene polymorphism seems to be important. Overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) within the promoter region or other regulatory sequences of cytokine genes. The majority of polymorphisms described are single nucleotide polymorphism (SNPs). The objective of this study was to analyze the genetic profile of Th1 and Th2 cytokines in 30 Iranian patients with CML and 40 healthy subjects.
Methods: In the patients and control subjects, the allelic and genotype frequencies were determined for the cytokine genes. All typing were performed by PCR-SSP assay. Allele and genotype frequencies were calculated and compared with those of normal controls.
Results: The results showed that the most frequent alleles in our patients were TGF-b TG/TG, IL-4 T at position -1089, C at position -590, T at position -33 and IL-10 A at position -1082. Whereas the following alleles - TGF-b CG/CG and IL-10 C at position -592 – were seen in much lower frequencies.
Conclusion: In conclusion, it could be suggested that the frequency of high producing TGF-b alleles and low producing IL-4 and IL-10 alleles in the CML patients is higher than the normal subjects.- انتشار مقاله: 13-05-1395
- نویسندگان: Ali Akbar Amirzargar,Morteza Bagheri,Ardeshir Ghavamzadeh,Kamran Alimoghadam,Farideh Khosravi,Mohammad Hossein Nicknam,Mandana Moheydin,Bita Ansaripour,Batul Moradi,Behrouz Nikbin
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: CML,Cytokine Gene Polymorphism
- چکیده:
- چکیده انگلیسی: Background:It has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing Chronic Myelogenous Leukemia (CML). In this regard, Th1 and Th2 cytokines and their gene polymorphism seems to be important. Overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) within the promoter region or other regulatory sequences of cytokine genes. The majority of polymorphisms described are single nucleotide polymorphism (SNPs). The objective of this study was to analyze the genetic profile of Th1 and Th2 cytokines in 30 Iranian patients with CML and 40 healthy subjects.
Methods: In the patients and control subjects, the allelic and genotype frequencies were determined for the cytokine genes. All typing were performed by PCR-SSP assay. Allele and genotype frequencies were calculated and compared with those of normal controls.
Results: The results showed that the most frequent alleles in our patients were TGF-b TG/TG, IL-4 T at position -1089, C at position -590, T at position -33 and IL-10 A at position -1082. Whereas the following alleles - TGF-b CG/CG and IL-10 C at position -592 – were seen in much lower frequencies.
Conclusion: In conclusion, it could be suggested that the frequency of high producing TGF-b alleles and low producing IL-4 and IL-10 alleles in the CML patients is higher than the normal subjects.- انتشار مقاله: 13-05-1395
- نویسندگان: Ali Akbar Amirzargar,Morteza Bagheri,Ardeshir Ghavamzadeh,Kamran Alimoghadam,Farideh Khosravi,Mohammad Hossein Nicknam,Mandana Moheydin,Bita Ansaripour,Batul Moradi,Behrouz Nikbin
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: CML,Cytokine Gene Polymorphism
- چکیده:
- چکیده انگلیسی: Background:It has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing Chronic Myelogenous Leukemia (CML). In this regard, Th1 and Th2 cytokines and their gene polymorphism seems to be important. Overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) within the promoter region or other regulatory sequences of cytokine genes. The majority of polymorphisms described are single nucleotide polymorphism (SNPs). The objective of this study was to analyze the genetic profile of Th1 and Th2 cytokines in 30 Iranian patients with CML and 40 healthy subjects.
Methods: In the patients and control subjects, the allelic and genotype frequencies were determined for the cytokine genes. All typing were performed by PCR-SSP assay. Allele and genotype frequencies were calculated and compared with those of normal controls.
Results: The results showed that the most frequent alleles in our patients were TGF-b TG/TG, IL-4 T at position -1089, C at position -590, T at position -33 and IL-10 A at position -1082. Whereas the following alleles - TGF-b CG/CG and IL-10 C at position -592 – were seen in much lower frequencies.
Conclusion: In conclusion, it could be suggested that the frequency of high producing TGF-b alleles and low producing IL-4 and IL-10 alleles in the CML patients is higher than the normal subjects.- انتشار مقاله: 13-05-1395
- نویسندگان: Ali Akbar Amirzargar,Morteza Bagheri,Ardeshir Ghavamzadeh,Kamran Alimoghadam,Farideh Khosravi,Mohammad Hossein Nicknam,Mandana Moheydin,Bita Ansaripour,Batul Moradi,Behrouz Nikbin
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Sexual dysfunction,Autologous,platelet,growth factor,pelvic irradiation
- چکیده:
- چکیده انگلیسی: Sexual dysfunction (SDF) is a common sequel to cancer treatment which affects the quality of life in women
treated with pelvic radiotherapy. The aim of this study was to evaluate the safety, symptom resolution and objective
improvement the injection of autologous platelet released growth factor (APRGF) for treatment of SDF in cited patients.
This prospective pilot study enrolled 10 cancer-free patients with SDF who underwent pelvic radiotherapy at least 5
years ago, randomly. Each patient was received 1-2 cc APRGF within four weeks and all patients were re-evaluated
at eight weeks and six months. CD34 immuno histochemistry and Masson’s trichrome staining were performed on
vaginal biopsy section for angiogenesis and fibrosis assay respectively. Sexual satisfaction after the injection of APRFG
was clinically difference and the entire patient had sexual satisfaction. In the patient’s follow-up, none of them needs
to repeat the treatment. Our results declared that APRGF injection was effective and symptoms were disappeared in
the entire patients. Significant objective improvements in vaginal diameter (mean before injection, 6.5 cm vs 7.1 cm
after injection) (p-value = 0.001) and vaginal flexibility (mean before treatment, 0.72 cm vs 1.85 cm after injection)
(P-value = 0.026) were observed. Characteristics of discharge before the injection in 60% of patients were included
dry vagina and 40% had mild discharge but after injection 40% of patients had moderate and also 60% had mild and
sufficient discharge (P-value= 0.190). Overally, our patients reported better sexual function and showed better vaginal
function indexes, after APRFG injection.- انتشار مقاله: 19-05-1397
- نویسندگان: Atefeh Samaie Nouroozi,Ashraf Alyasin,Ashraf Malek Mohammadi,Nili Mehrdad,Seyed Asadollah Mousavi,Mohammad Vaezi,Atoosa Gharib,Ardeshir Ghavamzadeh,Saeed Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: acute myeloid leukemia,Arsenic trioxide,Thalidomide,Vascular Endothelial Growth Factor (VEGF)
- چکیده:
- چکیده انگلیسی: Acute myeloid leukemia (AML) is a blood disorder characterized by uncontrolled proliferation of myeloid
progenitors and decrease in the apoptosis rate. The vascular endothelial growth factor (VEGF) promotes blood vessel
regeneration which might play important roles in development and progression of neoplasia. Our previous studies
focused on cytotoxicity and anticancer effects of arsenic trioxide (ATO) and thalidomide (THAL) as an anti-VEGF
compound in the AML cell model. ATO also affects regulatory genes involved in cell proliferation and apoptosis. The
aim of present study was to examine the effects of ATO and THAL alone and in combination on U937 and KG-1 cells
, with attention to mRNA expression for VEGF isoforms. Growth inhibitory effects was assessed by MTT assay and
apoptosis induction was determined by Annexin/PI staining. mRNA expression levels were evaluated by real-time
PCR. Our data indicated that ATO (1.618μM and 1μM in KG-1 and U937 cell lines respectively), THAL (80μM and
60μM) and their combination inhibited proliferation and induced apoptosis in our cell lines. mRNA expression of
VEGF (A, B) decreased while C and D isoforms did not show any significant changes. Taken together, according to
the obtained results, the VEGF autocrine loop could be a target as a therapeutic strategy for cases of AML.- انتشار مقاله: 14-10-1396
- نویسندگان: Mahnaz Mohammadi Kian,Saeed Mohammadi,Mahmoud Tavallaei,Bahram Chahardouli,Saharbano Rostami,Mahdi Zahedpanah,Ardeshir Ghavamzadeh,Mohsen Nikbakht
- مشاهده