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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesenchymal Stem Cells,Hepatocyte,Liver extract,Omentum tissue
- چکیده:
- چکیده انگلیسی: Objective(s): Mesenchyme is a type of undifferentiated loose connective tissue that is derived mostly from mesoderm. Recently, mesenchymal stem cells (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells, are of utmost importance for stem cell research. In this research, ability of the liver extract to induce differentiation of rat derived omentum tissue mesenchymal stem cells (rOT-MSCs) into hepatocyte cells (HCs) was investigated.
Materials and Methods: After isolation and confirmation of rOT-MSCs they were co-cultured with liver extract and hepatogenic differentiation was monitored. Expressions of mesenchymal stem cell markers were also analyzed via flow cytometry. Moreover, expressions of octamer-binding transcription factor-4 (Oct-4), Wilm's tumor suppressor gene-1 (WT-1), albumin (ALB), alpha fetoprotein (AFP), cytokeratin-18 (CK-18), and mRNAs were analyzed using RT-PCR on days 16, 18 and 21. ALB production was analyzed by immunocytochemistry and western blot. Furthermore, glycogen and urea production were determined via periodic acid-Schiff (PAS) staining and colorimetric assays respectively.
Results:The phenotypic characterization revealed the positive expressions of CD90, CD44 and negative expression of CD45 inrOT-MSCs. These cells also expressed mRNA of Oct-4 and WT-1 as markers of omentum tissue. Differentiated rOT-MSCs in presence of 6 µg/ml liver extract expressed ALB, AFP, CK-18, glycogen and urea as specific markers of HCs.
Conclusion: These observations suggest that liver extract is potentially able to induce differentiation of MSCs into hepatocyte lineage and can be considered an available source for imposing tissue healing on the damaged liver.- انتشار مقاله: 06-11-1393
- نویسندگان: Samaneh Solati Sarvandi,Mohammad Taghi Joghataei,Kazem Parivar,Maryam Khosravi,Arash Sarveazad,Nima Sanadgol
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Spinal cord injury,BBB,Chondroitinase ABC,Contusion,hADSCs
- چکیده:
- چکیده انگلیسی: Objective(s):Spinal cord injury (SCI) is one of the most serious clinical diseases and its treatment has been a subject of interest to researchers. There are two important therapeutic strategies in the treatment of SCI: replacing lost tissue cells through cells implantation and scar elimination. Therefore, in this study we used human adipose-derived stem cells (hADSCs) implantation and injection of Chondroitinase ABC.
Aim of present study was to answer to this question: which one is more efficient for Improvement of locomotor recovery after SCI in rat? Transplantation of hADSCs or injection of ChABC.
Materials and Methods: The spinal cord of rats was injured by contusion using a weight-drop at the level of T8-9, the hADSCs and Chondroitinase ABC were infused in to the spinal cord tissue after injury. BBB test was performed and recorded for each animal weekly for 8 weeks. After the 8th weeks, Serial cross-sections were stained with cresyl violet and examined under a light microscope and area of cavity in the spinal cord was measured.
Results: At 8th weeks after injection, hADSCs and ChABC significantly promote locomotor function (P<0.01) and spinal cords of hADSCs and ChABC group had cavities much smaller than those of the control group (P<0.001).
Conclusion: Results of the present study shows dealing with inappropriate neuro-inhibitory environment and glial scar by ChABC have equal role compare to cell therapy (with hADSCs) for improving motor function after SCI and this result in adoption of proper therapeutic strategies for SCI intervention is important.- انتشار مقاله: 02-07-1393
- نویسندگان: Arash Sarveazad,Mehrdad Bakhtiari,Asrin Babahajian,Atusa Janzade,Ali Fallah,Fatemeh Moradi,Mansoureh Soleimani,Mohammadreza Younesi,Farjam Goudarzi,Mohammad Taghi Joghataei
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Fecal incontinence,Constipation,Pediatrics,Antegrade Colonic Enema
- چکیده:
- چکیده انگلیسی:
Background
Some controversy exists about the role of cecostomy in the management of fecal disorders. The present meta-analysis aims provide a comprehensive evaluation on the role of cecostomy on management of fecal incontinence and constipation in children.
Materials and Methods
An extensive search was performed on the Medline, Embase, Scopus, and Web of Science until July 2017. Two independent researchers screened the title and abstracts of the studies and then relevant studies were included. Finally, pooled effect size was presented as standardized mean difference (SMD) or pooled prevalence with 95% confidence interval (95% CI).
Results
14 articles were entered (740 children). The success rate of cecostomy in management of fecal disorders was 90.6% (success rate=90.6%; 95% CI: 86.4 to 94.2). The most common side effects of this technique include granulation tissue formation (29.6%), cecostomy tube leakage (8.5%), tube dislodgement (7.0%), and tube site infection (2.3%).
Conclusion
The results of the present meta-analysis show that the cecostomy is safe and an effective technique in the management of fecal disorder in children. However, the findings presented on the eligible articles have have shown a low level of evidence and it is suggested that clinical trials should be conducted in this field.
- انتشار مقاله: 18-11-1397
- نویسندگان: Asrin Babahajian,Jebreil Shamseddin,Arash Sarveazad,Mahmoud Yousefifard
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Mice,Brain,Methylphenidate,Ritalin
- چکیده:
- چکیده انگلیسی: Background
Methylphenidate (MPH) is commonly prescribed for children who have been diagnosed with attention deficit hyperactivity disorder (ADHD); however, the action mechanisms of methylphenidate have not been fully elucidated. Studies have shown a relationship between apoptosis signaling pathways and psychiatric disorders, as well as therapeutic targets for such disorders. So, we examined the effects of chronic methylphenidate administration on the brain of mice.
Materials and Methods
Animals were administered MPH at doses of 2, 5 and 10 mg/kg for 60 days. At the age of three months and in estrous phase, brian tissues were removed and washed in cold phosphate-buffered saline and some of them were frozen at -80oC for Western blot analysis. We measured the levels of pro-apoptotic protein, Bax and anti-apoptoticprotein, Bcl-2, in the brain of neonate female Balb/c mice. The rest of the brains were fixed in formalin (10% phosphate-buffered, pH = 7.4). Then samples were embedded in paraffin according to routine histologic procedures.
Results: Our results showed that MPH with a dose of 10 mg/kg causes a considerable increase in the level of the Bax protein as compared with other groups. In contrast, in the partial cortex of female mice under treatment with high dose of MPH (10 mg/kg) could less Bcl2 levels as compared with 5 mg/kg MPH. However, 5 mg/kg MPH have a significant effect on Bcl2 levels compare with each of mentioned doses (P<0.05).
Conclusion
Our results suggest that long-term administration of MPH in the mouse brain had influence on the cascade of apoptosis and its effects, depends on dose rate.- انتشار مقاله: 28-12-1396
- نویسندگان: Amirreza Katebi,Fereshteh Golab,Gelareh Vahabzadeh,Arash Sarveazad,Nasim Goodarzi,Simin Fazelipour,Mahmood Barati,Mansoureh Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Emergency Service,Pediatrics,Sensitivity and Specificity,Decision support systems
- چکیده:
- چکیده انگلیسی: Background: The present study assesses independent predictors of clinically important traumatic brain injury (ciTBI) in order to design a prognostic rule for identification of high risk children with mild head injury. Materials and Methods: In a retrospective cross-sectional study, 3,199 children with mild traumatic brain injury (TBI) brought to emergency ward of three hospitals in Tehran, Iran were gathered, from April 2014 to April 2016. The associations between probable predictors of ciTBI in children with mild TBI were assessed and a prediction rule for identification of high risk children in need of computed tomography (CT) scan was designed based on a stepwise multivariate logistic regression. Results: 592 (18.5%) children had ciTBI. History of loss of conciseness (odds ratio [OR]=3.0; p<0.0001), underlying disease (OR=3.6; p=0.002), Glasgow coma scale (GCS) score equal to 14 (OR=40.6; p<0.0001), altered mental status (OR=19.1; p<0.0001), need for intubation (OR=27.4; p<0.0001), presence of vomiting (OR=7.3; p=0.001), and sign of basilar skull fracture (OR=25.9; p=0.007), were the most important prognostic factors of ciTBI in children. Pediatric traumatic brain injury prognostic rule (PTBI prognostic rule) was designed based on these predictors. PTBI prognostic rule had an area under the curve of 0.93, a sensitivity of 100.0%, a specificity of 73.0% and a proper calibration (slope=0.97 and intercept=0.006) in identification of ciTBI. Conclusion: The present study showed that a few of children with mild TBI have ciTBI. Therefore, CT scans are not necessary in all of these children. Using PTBI prognostic rule can reduce the number of unnecessary CT scans.
- انتشار مقاله: 27-09-1395
- نویسندگان: Babak Nakhjavan-Shahraki,Mahmoud Yousefifard,Alireza Oraii,Arash Sarveazad,Mohammad Javad Hajighanbari,Saeed Safari,Alireza Baratloo,Abbas Tafakhori,Mostafa Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Breast cancer,Survival,Metastasis,clinical stage
- چکیده:
- چکیده انگلیسی: Introduction: Currently breast (BC) cancer is a serious medical problem in all countries of the world. Survival
depends on many factors. The present study focused on 5-year survival and its related factors in patients with BC in
Iran. Material and methods. The present analytical retrospective study was performed (from March 2010 until March
2015) on patients with BC followed for at least 6 months. The main variables assessed were tumor size, grade of lymph
node involvement, metastasis, stage, history, human epidermal growth factor receptor expression, and tumor origin.
Analysis of survival was accomplished using the Kaplan- Meier method. Results: Some 351 (80.2%) of the total of
438 individuals had unilateral and 87(19.8%) had bilateral cancer, 28 (35.6%) of the latter being synchronous and
56(64.4%) metachronous. Mean duration of follow-up was 47.44±28.19 months, during which 61 (17.3%) patients
with unilateral and 18 with bilateral cancer eventually died. The 5-year survival rate in patients with unilateral BC was
significantly higher than those with bilateral BC (Log-rank Test chi2= 3.11, p=0.032). In addition, with metachronous
cases, the survival rate was 64.2% in comparison with 51.6% for synchronous BCs. Survival rate was significantly
(p value =0.038) higher with metachronous than with synchronous cancers (Log-rank Test chi2=3.54, p=0.038). The
highest survival rate was reported for BCs originating from lobule tissue and the lowest rate examples of interstitial
tissue origin (Log-rank Test chi2=11.54, p=0.0001). Patients with earl stage lesions (M1) survived longer than with
other stages (Log-rank Test chi2= 9.55, p=0.001). Conclusion: In this study, most women with BC had a positive
family history and were married. The 5-year survival rate was lower with advanced stages of cancer. According to our
findings, survival rates might improve if patients undergo screening and diagnosis is made at an early stage of the disease.- انتشار مقاله: 22-12-1396
- نویسندگان: Arash Sarveazad,Asrin Babahajian,Jebreil Shamsadin,Mansour Bahardoust
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,CD34,Homeodomain protein TGIF2LX,Ki-67,Transforming growth factor-beta (TGF-β)
- چکیده:
- چکیده انگلیسی:
Background: TGIF2LX (transforming growth factor beta-induced factor 2 like, X-linked) is a homeodomain (HD) protein that has been implicated in the negative regulation of cell signaling pathways. The aim of this study was to investigate the possible functions of TGIF2LX in colon adenocarcinoma cells. Methods: The human SW48 cell line was transfected with cDNA for the wild-type TGIF2LX gene and gene/protein over-expression was confirmed by microscopic analysis, real time RT-PCR and Western blotting techniques. In vitro cell proliferation was evaluated by MTT and BrdU assays. After developing a colon tumor model in nude mice, immunohistochemical (IHC) staining of tumor tissue was carried out for Ki-67 (proliferation) and CD34 (angiogenesis) markers. To predict potential protein partners of TGIF2LX, in-silico analysis was also conducted. Results: Obtained results showed over-expression of TGIF2LX as a potential transcription factor could inhibit either proliferation or angiogenesis (P<0.05) in colon tumors. In-silico results predicted interaction of TGIF2LX with other proteins considered important for cellular development. Conclusions: Our findings provided evidence of molecular mechanisms by which TGIF2LX could act as a tumor suppressor in colon adenocarcinoma cells. Thus, this gene may potentially be a promising option for colon cancer gene-based therapeutic strategies.- انتشار مقاله: 23-11-1395
- نویسندگان: Abolfazl Akbari,Shahram Agah,Mansour Heidari,Gholam Reza Mobini,Ebrahim Faghihloo,Arash Sarveazad,Alireza Mirzaei
- مشاهده