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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Detoxification,Mycotoxin,Penicillium,Citrinin,Cold Atmospheric Pressure Plasma
- چکیده:
- چکیده انگلیسی: Mycotoxins such as Citrinin are small toxic molecules produced by a great variety of microorganism, which encompass several classes of secondary metabolites with no common chemical structure or mode of action. Citrinin produces by different species of Penicillium. Today, the cold atmospheric pressure plasma (CAPP) method has a potential for mycotoxin detoxification. In this study, a new and promising approach involving the use of cold atmospheric pressure plasma is considered, which may overcome multiple weaknesses associated with the classical methods.
In this study, samples included wheat, corn; rice and Barley were obtained from northern parts of Iran (Lahijan). Initially, the output of the power supply was set to 50 kV, 100 watts, and the electron frequency of 30 kHz (optimal conditions to effective plasma). Then, the effect of cold atmospheric jet plasma (argon) on gas was investigated in 30, 60 and 360 seconds (gas flow rate of 6 liters per minute).
The results showed us that the cold atmospheric pressure plasma method effectively production pure mycotoxins. The rate of various mycotoxins destruction varies according to their structure. It was also shown in the study that the combination of mycotoxins in foods would reduce the plasma's effect, but not prevent them from degrading. Finally, in this study, descendants in all samples were performed successfully.
The atmospheric pressure plasma method has a natural biodegradation effect and has the least negative effect on food products in comparison with conventional methods of disinfection of food products in addition to the detoxification characteristics.- انتشار مقاله: 24-06-1396
- نویسندگان: Narges Vaseghi,Mansour Bayat,Arash Chaichi Nosrati,Mahmood Ghorannevis,Jamal Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Zearalenone toxin,Mycoprotein,Fusarium solani
- چکیده:
- چکیده انگلیسی: Fusarium is a fungus that is commonly found as saprophytes with parasitic life on some living organisms. Mycoprotein or fungus proteins with physical and chemical features are known as food in recent years. When they are involved in temperature and wet stress, release materials such as mycotoxins or fungus toxins remained in food. Zearalenone and dependent compounds have distinct role in many fungus toxicity and beings especially despite acute toxicity. Also the global allowed rate of zearalenone in foods is 30-1000 nano gr/ppb. The submerged culture environment to produce fungus biomass was Vogel basic medium.Then, the produced fungus biomasses were harvested at 4500rpm for about 20 minutes by centrifugation, washed and rinsed twice and desiccated overnight in room temperature. The dry weight biomass was measured and used in order to measure protein rate extracts for toxin estimating based on producer instructions. Then, 50ml substrate was added and after 5 minutes stopper enzyme was added. Finally, the zearalenone toxin amount was measured by ELISA reader system. The results show that after nitrogen resource, the carbon resource play the second role in production and increase of toxin rate in mycoprotein biomass extracts. Verifying the optimum submerged environment in which all optimal conditions of each steps were applied (the first priority of optimal environment include 0.25% of Starch originated carbon resource and 0.25% of Urea originated nitrogen resource) showed the rate of protein production was 0.642% that is have more increased about 0.207% in relation to basic submerged environment. In this environment the amount of zearalenone toxin was 0.99 ppb/gr. In the other way, when we use 2.64% of Rice bran as replacement carbon resource, using 0.75% of Urea in compare with 0.50% of Soy bean peptone as nitrogen resources we could observe an increase of toxin rate in biomass about 0.66 %. While the amount of Urea reduced to 33% and even we use 0.25% of Starch instead of Rice bran, the toxin in biomass reduced into 71% and reached to 0.99 ppb/gr. When we use 2.30% of Meat peptone, applying 0.50% of Urea in compares with 0.50% of Soy bean peptone increase up to 66% of zearalenone toxin in biomass and reached to 0.77 ppb/gr.
- انتشار مقاله: 03-12-1391
- نویسندگان: Fereshteh Jafarian,Arash Chaichi Nosrati,Leila Modiri,Mohammad Faezi Ghasemi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Northern iran,Aspergillus,Aflatoxin,pattern
- چکیده:
- چکیده انگلیسی: Objective: Aflatoxin G1 and G2, are highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus fungi sampling was done according to "CBS" instructions from indoor and outdoor stations. Methods: In HPLC measuring, we first purified our sample of all Aflatoxins, We used imonoaffinty columns, Finally the device compared average of the results of the samples curve areas with average of the results of standard curve areas and showed the effective material value based on ppb. Geographical distribution of 107 mentioned samples and The frequency of subgenuses in different regions, The minimum, average and maximum amount of toxins, The average amount of produced toxin in culture medium were determined. Results: In comparison, the average of aflatoxin G1 and G2with HPLC method in biomass the highest examined amount is in the range of -0/5 -0/5 ppb which is produced by fungi such as A. niger, A. flavus, A. fumigatus. In biomass, the correlation between aflatoxin G1 and G2 measured by HPLC method isn’t any reverse relation and meaningful numeral differences. In culture medium, checking the average value of aflatoxin G1 and G2which is measured by HPLC method, we can see a correlation and there is a meaningful numeral difference.
- انتشار مقاله: 14-06-1394
- نویسندگان: Zohre Zohari Moafi,Arash Chaichi Nosrati,Omid Lakzaie Azar
- مشاهده