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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Spectrophotometry,Transition metals,Fluorescence spectroscopy,Dipodal chelator,Zn2+ Sensor,Potentiometric
- چکیده:
- چکیده انگلیسی: A novel polyfunctional dipodal ligand, L = N,N'-bis[2-[(2-hydroxy-1-naphthyl)methyleneamino]ethyl]propanediamide (DOTA2HNAP) was developed and characterized through elemental and spectral analyses. The complexation behavior of the ligand was investigated with Co2+, Cu2+, and Zn2+ metal ions by potentiometric and spectrophotometric methods in the H2O-DMSO mixture (99:1) at µ = 0.1M KCl and 25 ± 1 °C. Four protonation constants for –OH of naphtholate groups and –N of imine were determined for the ligand. The ligand forms monomeric complexes of ML type with the metal ions, where coordination occurs through N-imine and O-naphtholate donors (N2O2). In the case of a complex of copper, an additional species, MLH-2, was formed due to ionization of the amide groups in a higher pH. The minimum energy structures of the metal complexes in solution have been obtained through molecular modeling studies by using the semi-empirical/ PM3 method. The photophysical properties of DOTA2HNAP were investigated in the presence of a wide range of biologically relevant metal ions. The fluorescence emission of the ligand at 450 nm (λex = 361 nm) exhibited a remarkable enhancement with Zn2+ ions (1 equivalent) at physiological pH amongst all metal ions. Such behavior enables the ligand to be considered as a suitable model for the detection of Zn2+ towards environmental applications.
- انتشار مقاله: 31-02-1397
- نویسندگان: Amit Gupta,Vijay Dangi,Minati Baral,B Kanungo
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Metastasis,Leukemia,Tumor suppressor genes,carcinogenic
- چکیده:
- چکیده انگلیسی: Objective: The transformation in cells at genetic levels stimulatesthe proliferation of cancer. The current review highlights the role of miRNA in management of cancer by altering processes of body at cellular levels. Methods: A deep research on the literature available till date for miRNA in cancer was conducted using various medical sites like PubMed, MEDLINE from internet and data was collected. The articles were majorly preferred in English language. Results: The development of normal cells into cancerous cells is a multivalent procedure highlighting numerous responsible factors. During the progression of cancer, the role of oncogene and tumor suppressor genes outshines at different levels of tumorogenesis. Metastasis poses highest threat in cancer progression and fabricates obstacles to clinicians and researchers in preventing formation of tumor on secondary sites. The mesenchymal-epithelial transition (MET) and epithelial mesenchymal transition (EMT) induce dissemination and ultimately progression of cancer. Conclusion: A comprehensive knowledge of the altered genes and the mechanism by which they induce formation of tumor is essential as they contribute in proliferating cancer at various stages, aggravating clinical symptoms. Hence miRNAs can be efficiently employed as an emerging treatment therapy for cancer.
- انتشار مقاله: 30-09-1398
- نویسندگان: Tapan Behl,Chanchal Kumar,Rashita Makkar,Amit Gupta,Monika Sachdeva
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Cisplatin,MDM2-p53 interaction,colon and prostate cancer cells
- چکیده:
- چکیده انگلیسی: Objective: To study the effect of RITA (MDM2-p53 interaction inhibitor) and its action along with genotoxic drug cisplatin was evaluated on COLO-205 colon cancer and PC-3 prostate cancer cells. Method: Various in-vitro parameters to determine cytotoxic and apoptotic potential of RITA with genotoxic drug cisplatin were evaluated. The potentiation of cytotoxic effect was evaluated using MTT assay and colony forming assay, mechanism of cell death by Etbr/AcO assay and the mechanism of apoptosis was determined by caspase-3 release assay. Results: The findings from MTT confirmed the best possible potent combination of 5+5µM and 10+10µM concentration of Cisplatin and RITA respectively. These combinations were further evaluated for its chemo sensitizing effect which confirmed the significant reduction in number of colonies in combination as compared to monotherapy. Also, the results of Etbr/AcO assay were in line with the colony forming assay. For apoptotic activity, it was noted that increasing the concentration of cisplatin and RITA (10µM), did not affect much to apoptotic activity and was found to be equally effective to that of low dose (5µM) concentration. The same results were seen in Caspase-3 release effect on both the cell lines. Conclusion: Our present study provides compelling evidence that pharmacological activation of the p53 by blocking the MDM2–p53 interaction is a promising cancer therapeutic strategy and using RITA in combination with Cisplatin not only decrease the toxic effect of Cisplatin by decreasing its dose but also increasing the apoptotic effect, warrants clinical evaluation on both colon and prostate cancer.
- انتشار مقاله: 29-10-1397
- نویسندگان: Amit Gupta,Tapan Behl,Hem Raj Heer,Rahul Deshmukh,Pyare Lal Sharma
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Ficus benghalensis, Ficus racemose, Antimicrobial,NDV,IBD
- چکیده:
- چکیده انگلیسی: Background and aims: Medicinal plant products are considered to be an effective candidate against the number of viral diseases as generally observed or reported in developing countries. As per the literature, secondary metabolites (i.e. Alkaloids, flavonoids, saponins etc.) are reported in medicinal plant products and showed its antiviral properties. In this study, our group focused on those medicinal plants especially roots of Ficus benghalensis and Ficus racemosa related to New castle Disease Virus (NDV) and Infectious Bursal Disease (IBD) having in vitro antiviral activity. These studies were conducted on the human peripheral blood mononuclear cells (PBMC).
Methods: For antimicrobial studies, different medicinal plant products especially roots of Ficus benghalensis and Ficus racemosa were collected from Vidya Pratishthan’s garden, School of Biotechnology, Baramati. These medicinal plant leaves are used in the form of aqueous extract and determined its anti-microbial activity against poultry viruses (i.e. NDV and IBD) in PBMC and determined proliferation assay, Th1 (TNF alpha) cytokine production and CD14 monocyte surface marker.
Results: Three medicinal plant aqueous extracts showed noteworthy antimicrobial activity with respect to decline in proliferation assay, TNF alpha production and CD14 monocyte surface marker in human PBMC as compared to control.
Conclusion: Some of the medicinal plant products have shown antimicrobial activity. Further immunobiological research is mandatory to elucidate the most active constituents that are present in these aqueous extracts which may be useful or target molecule in the development of new effective and safe antiviral agents.- انتشار مقاله: 05-04-1395
- نویسندگان: Amit Gupta,Sushama R Chaphalkar
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Immunosuppressive,Anti-inflammatory,Mangifera indica,Hepatitis B vaccine
- چکیده:
- چکیده انگلیسی: Background: There is a worldwide interest in searching for potential and effective medicinal plant candidates against various diseases or disorders. Till now, there are number of anti-inflammatory drugs like NSAIDs are available which showed various adverse effects in our body. To reduce these adverse effects, some of medicinal plants have been experimentally validated. Aim: The aim of our immunopharmacological study is to investigate the immunosuppressive and anti-inflammatory activity of aqueous leaves extract of Mangifera indica in human peripheral blood mononuclear cells (PBMC) extracellular against hepatitis B vaccine antigen (HBsAg) Methods: In this study, aqueous leaves extract were collected from Mangifera indica and determined its effect on total blood counts (using forward and side scatter) and monocyte CD14 FITC surface markers using flow cytometry. In addition, the effect of aqueous leaves extract on nitric oxide (NO) production from PBMC cell culture supernatant and also estimate its proliferation assay using Concanavalin A (Con A, 0.5 mg/ml, 50 µl). Data analysis was performed using BD cell Quest Pro software for flow cytometric analysis and one way ANOVA test (Boniferroni multiple comparison test). Results: The results displayed that aqueous leaves extract of Mangifera indica showed dose dependent decline in blood counts (increased in side scatter and slightly decreased in forward scatter), monocyte CD14 FITC surface marker; Con A proliferation and nitric oxide production from cell culture supernatant in human PBMC. Conclusion: Overall, the results claimed that aqueous leaves extract of Mangifera indica at higher doses showed immunosuppressive and anti-inflammatory activity.
- انتشار مقاله: 24-06-1394
- نویسندگان: Amit Gupta,Sushama R Chaphalkar
- مشاهده