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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Fisheries Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial activity,sea cucumber,Holothuria parva
- چکیده:
- چکیده انگلیسی: Sea cucumbers are the most important medicinal group among echinoderms. The presence of a wide range of natural bioactive compounds from sea cucumbers has been confirmed in many studies. This study was aimed to evaluate the antibacterial activities of ethanol, methanol and acetone extracts from sea cucumber Holothuria parva. Sea cucumber were collected during low tide from Ola village, Bushehr, Iran. Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of the extracts were determined against five human pathogens. The different components in Holothuria parva were determined by FTIR (Fourier Transform Infrared Spectroscopy) method. All of the crude extracts were shown antimicrobial activity against E.coli , P.aeroginosa and E. fecalis. Also, methanol and ethanol extracts had inhibitory and bactericidal activities on growth of the tested bacteria, respectively (p< 0.05). The FTIR spectra showed the presence of five components such as glycerol, gluconic acid, ouabain, spectinomycin and capreomycin in Holothuria parva. Our results showed that sea cucumber could be an appropriate marine source for antimicrobial components. Further in vivo investigations need to be carried out on its potential application in other aspects of medicine.
- انتشار مقاله: 16-01-1395
- نویسندگان: Hadi Ebrahimi,Amir Vazirizadeh,Iraj Nabipour,Akram Najafi,Saeed Tajbakhsh,Mahmoud Nafisi Bahabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,Apoptosis,Melanoma,Turbo coronatus,Gel filtration
- چکیده:
- چکیده انگلیسی: Objective: A variety of approaches such as surgery, chemotherapy, radiotherapy, hormonal therapy and
immunotherapy are used to treat melanomas, but unfortunately in most case, the response is very weak and often side
effects are serious. This study concerns selective toxicity of an extract of Turbo coronatus on cells and mitochondria from
a syngeneic mouse model of melanoma. Methods: Cells and mitochondria isolated from extra tumoral and melanoma
tissues were exposed toa T. coronatus crude extract and fractions obtained by gel-filtration chromatography and assayed
for mitochondrial and cellular parameters. Result: Crude extract (375, 750 and 1,500 μg/ml) and fraction 1; F1; (275,
550 and 1100 μg/ml) of T. coronatus extract induced a significant (p<0.05) increase of the reactive oxygen species
(ROS) level, swelling of mitochondria, collapse of mitochondrial membrane potential (MMP), release of cytochrome
c and caspase-3 activation only in the mitochondria and cells obtained from melanoma but not extra tumoral tissues. In
addition, the F1 fraction decreased the percentage of viable cells and induced apoptosis in melanoma cells. Conclusion:
For the first time we could demonstrate that the F1 fraction of a T. coronatus extract, selectively induces ROS mediated
cytotoxicity by directly targeting mitochondria in melanoma tissues and it may be a suitable candidate for novel drug
treatment of malignant melanomas.- انتشار مقاله: 16-12-1396
- نویسندگان: Fatemeh Zangeneh,Amir Vazirizadeh,Mohammad Reza Mirshamsi,Amir Fakhri,Mehrdad Faizi,Jalal Pourahmad
- مشاهده