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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Acellular matrix, Burn wounds, Fetal skin extract, Honey, Wound healing
- چکیده:
- چکیده انگلیسی: Treatment of infected burn wounds remains a challenge in burn units. Silver-sulfadiazine (SSD) is the most commonly used topical antimicrobial agent in managing these wounds. We aimed to accelerate the healing of burn wounds by combined application of ovine acellular peritoneal matrix (OAPM), honey (H), and ovine fetal skin extract (OFSE). Sixty-four standardized burn wounds were created on the dorsum of 16 rats and were subsequently inoculated with Staphyloccocus aureus and Pseudomonas aeruginosa. After 48 hr, the wounds were surgically debrided and received either physiologic saline (control group) or SSD, OAPM+SSD, OAPM+H+SSD, OAPM+H+OFSE+SSD. The healing wounds were evaluated for size, bacterial counts, histopathology, and biomechanical properties on days 3, 7, 14, 21, and 28 after surgery. All treatments had effectively reduced the level of S. aureus and P. aeruginosa on wounds compared to the control group by day 3 and 7. The wounds treated with combined application of OAPM+H+OFSE+SSD demonstrated considerable inflammation reduction, fibroplasia, complete re-epithelialization, and wound contraction together with significantly lesser scar tissue formation. Treatment with OAPM+H+OFSE+SSD showed superior biomechanical properties of the healing wounds. The findings suggested that the synergistic effect of dressing the wounds with OAPM, H, and OFSE was a very effective approach in accelerating the healing process of the experimentally induced infected full-thickness burn wounds in rats.
- انتشار مقاله: 01-06-1397
- نویسندگان: Behrouz Nikahval,Ahmad Oryan,Parastoo Memarian,Amir Kamali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: sorafenib,Hepatocellular carcinoma,human placenta Mesenchymal stem cell,animal model
- چکیده:
- چکیده انگلیسی: Background: Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the third leading cause
of cancer-related death. sorafenib is used as a standard therapy to treat HCC. mesenchymal stromal cells (MSCs)
have also been used to suppress HCC. Here we investigate the development of a xenograft model of liver cancer to
study the homing of hpMSC-GFP cells, tumor kinetics and molecular characterizations of HCC. Methods: To create
xenograft models of HCC, HepG2 cell lines were inoculated into the flanks of 9 nude mice bilaterally. Animals were
then divided into three groups: the first group received hpMSC-GFP systemically, the second received intra-tumoral
hpMSC-GFP and the third received PBS. The first two groups were sacrificed after 72 hours of MSCs injection but
the third group was followed up for forty days. One tumor from each animal was then transferred to formalin buffer
for H&E staining and immunohistochemistry analysis (KI67 and CD34), and the other tumor was used for ex-vivo
imaging. Blood samples were taken from all subjects before sacrificing them. Results: Histopathological fidelity of
heterotopic HePG2 xenograft models to human HCC tumors was demonstrated. Biochemical evaluation suggested
the health of the animal’s liver and kidneys. Ex-vivo imaging illustrated homing of more hpMSC-GFP cells in tumor
tissues derived from the group receiving intra-tumoral hpMSC-GFP. Conclusion: A standard method was used to
inoculate tumor cells and the intervention was shown to be safe to liver and kidneys. Local injection of MSCs can be
used as cell therapy to fight neoplasms.- انتشار مقاله: 19-09-1396
- نویسندگان: Saieh Hajighasemlou,Saeedreza Pakzad,Jafar Ai,Samad Muhammadnejad,Milad Mirmoghtadaei,Faezeh Hosseinzadeh,Safoora Gharibzadeh,Amir Kamali,Akbar Ahmadi,Javad Verdi
- مشاهده