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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,Apoptosis,Melanoma,Turbo coronatus,Gel filtration
- چکیده:
- چکیده انگلیسی: Objective: A variety of approaches such as surgery, chemotherapy, radiotherapy, hormonal therapy and
immunotherapy are used to treat melanomas, but unfortunately in most case, the response is very weak and often side
effects are serious. This study concerns selective toxicity of an extract of Turbo coronatus on cells and mitochondria from
a syngeneic mouse model of melanoma. Methods: Cells and mitochondria isolated from extra tumoral and melanoma
tissues were exposed toa T. coronatus crude extract and fractions obtained by gel-filtration chromatography and assayed
for mitochondrial and cellular parameters. Result: Crude extract (375, 750 and 1,500 μg/ml) and fraction 1; F1; (275,
550 and 1100 μg/ml) of T. coronatus extract induced a significant (p<0.05) increase of the reactive oxygen species
(ROS) level, swelling of mitochondria, collapse of mitochondrial membrane potential (MMP), release of cytochrome
c and caspase-3 activation only in the mitochondria and cells obtained from melanoma but not extra tumoral tissues. In
addition, the F1 fraction decreased the percentage of viable cells and induced apoptosis in melanoma cells. Conclusion:
For the first time we could demonstrate that the F1 fraction of a T. coronatus extract, selectively induces ROS mediated
cytotoxicity by directly targeting mitochondria in melanoma tissues and it may be a suitable candidate for novel drug
treatment of malignant melanomas.- انتشار مقاله: 16-12-1396
- نویسندگان: Fatemeh Zangeneh,Amir Vazirizadeh,Mohammad Reza Mirshamsi,Amir Fakhri,Mehrdad Faizi,Jalal Pourahmad
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,Apoptosis,Venom,Colorectal cancer,Naja naja oxiana
- چکیده:
- چکیده انگلیسی:
Objective: To investigate the selective effect of Naja naja oxiana crude venom and its fractions on human colorectal cancer mitochondria to activate apoptosis signaling. Methods: Cells and mitochondria isolated from human cancerous and normal colorectal tissues exposed to N. oxiana crude venom and its fractions obtained from size-exclusion chromatography and then mitochondrial parameters related to up-stream cell death signalling such as reactive oxygen species formation, MMP, mitochondrial swelling, cytochrome c release and ATP content as mitochondrial parameters and activation of caspase3 and finally apoptosis/necrosis % were then assayed as cellular parameters. Result: Our findings indicated that crude venom (15, 30 and 60 μg/ml) and fraction 3; F3; (10, 20 and 40 μg/ml) of N. Oxiana venom induced a significant (p<0.05) increase of reactive oxygen species level, swelling of mitochondria, collapse of mitochondrial membrane potential (MMP), release of cytochrome c, activated caspase3 and decrease ATP content only in colon cancer tissue group but not from the healthy colon tissue group. Our results also showed that fraction 3 of venom decreased the percentage of viable cells and induced apoptosis in cancerous colorectal cells. Conclusion: F3 fraction of N. Oxiana venom is a suitable candidate for further studies as a new drug treatment of colorectal cancer due to its high capacity for induction of apoptosis signaling via mitochondrial pathway.- انتشار مقاله: 14-02-1396
- نویسندگان: Amir Fakhri,Ramesh Omranipour,Sara Fakhri,Mohammadreza Mirshamsi,Fatemeh Zangeneh,Hossein Vatanpour,Jalal Pourahmadjaktaji
- مشاهده