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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Titanium dioxide,nanoparticle,Doxorubicin,Drug Delivery
- چکیده:
- چکیده انگلیسی: Many chemotherapeutics used for cancer treatments show systemic toxicity to the normal tissues. Using nanoparticles (NPs) improves drug delivery efficiency and decreases the side effects. The aim of this study was to load doxorubicin (DOX) on TiO2 NPs for its potential role in enhancing the anticancer efficacy of DOX while reducing its side effects. At first, for improving the dispersibility of TiO2 NPs in water, the polyethylene glycol (PEG) with two MWs (1000 and 4000 kDa) was used for wrapping the surface of TiO2 NPs. DOX was loaded on the TiO2 NPs by forming complexes with titanium, to construct TiO2-PEG-DOX NPs. The effects of various weight ratios of DOX to TiO2 on the loading efficiencies of NPs were assessed. The stability of these NPs in 2 different pH values (7.4 and 5) was evaluated. At the end, the cytotoxicity of TiO2-PEG-DOX was compared with free DOX against MCF-7 cell line. The formation of a thin layer of PEG around the TiO2 NPs was confirmed through thermo gravimetric analysis and transmission electron microscopy techniques. Fluorescence scanning results showed the complex formation between DOX and TiO2-PEG. The loading efficiency of DOX in TiO2-PEG1000 was 74% and in TiO2-PEG4000 was up to 85%. These complexes were stable at different pH values for long time (one month). The viability percent of cancerous cells expose to TiO2-PEG-DOX was lower than free DOX after 48 hours. The characteristics of TiO2-PEG-DOX show that this drug delivery system is a promising strategy for future clinical practice.
- انتشار مقاله: 12-01-1398
- نویسندگان: Zahra Sobhani,Reyhaneh Khademi,Mohammad Ali Behnam,Amin Reza Akbarizadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: The aim of this study was to evaluate the effect of the hexyl acrylate conjugated polyamidoamine (PAMSM) dendrimer on different cell lines in the presence and absence of serum. Although the positively charged dendrimers including PAMAM has been considered as efficient gene carriers, their cytotoxicity is a major barrier for their clinical applications. The conjugation of hexyl acrylate on PAMAM dendrimer was carried out to improve the hydrophilic-hydrophiobic balance of the dendrimer as well as the modulation of the positive charge on the dendrimer structure. The cytotoxicity of PAMAM derivatives was evaluated using MTT test on HepG2, MCF-7 and A549 cell lines in the presence and absence of serum. The results revealed that the modified PAMAM dendrimers induced less toxic effects on the cell lines in the presence of serum. Therefore, conjugation of acrylate on the PAMAM structure could be an efficient way to reduce the dendrimer toxicity allowing the administration of more dendrimer for gene delivery.
- انتشار مقاله: 04-12-1397
- نویسندگان: Mohammad Ashkan Naderian,Amin Reza Akbarizadeh,Akram Jamshidzadeh,Ali Dehshahri
- مشاهده
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: The xenobiotics-induced liver injury is a major clinical complication. Hence, finding hepatoprotective agents could have clinical value. Herbal medicines are a major source of biologically active chemicals which could be applied as hepatoprotective agents. The current study was designed to assess the hepatoprotective properties of Avicennia Marina (AM) extract and its different fractions. In vivo, the hepatoprotective effect of AM total extract against CCl4-induced acute liver injury was evaluated in rats, and a series of histopathological, biochemical, and oxidative stress parameters were monitored. In vitro, the protective effect of AM extract fractions (Petroleum ether, Chloroform, Ethyl acetate, and Ethanol) was evaluated on human liver hepatoma cells (HepG2). Severe elevation in serum level of liver injury biomarkers, along with liver tissue histopathological changes, lipid peroxidation, and liver tissue glutathione depletion were detected in CCl4-treated rats. On the other hand, CCl4-induced toxicity was evident in vitro by significant cell death. It was found that AM extract provided significant protection against CCL4 toxicity in vivo by decreasing serum biomarkers of liver injury and tissue markers of oxidative stress. In vitro, the protective effect of AM extract fractions (Chloroform, Ethyl acetate, and Ethanol) was evident as these fractions significantly decreased CCl4 cytotoxicity. As AM extract exhibited significant suppression of oxidative stress markers, its antioxidant effect could play a significant role in its hepatoprotective properties.
- انتشار مقاله: 29-08-1396
- نویسندگان: Omid Farshad,Reza Heidari,Hamidreza Mohammadi,Amin Reza Akbarizadeh,Mohammad M. Zarshenas
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,oxidative stress,hepatotoxicity,CCl4,Anise seed,HepG2 cells
- چکیده:
- چکیده انگلیسی: Objective(s): Protective effects of different extracts and essential oil from Pimpinella anisum L. seeds were examined against carbon tetrachloride (CCl4)-induced toxicity. The parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. In the in vitro model of this study, markers such as cell viability, cellular reduced and oxidized glutathione and lipid peroxidation in HepG2 cells were evaluated.
Materials and Methods:Human liver cancer cell line HepG2 and male Sprague-Dawley rats were treated with extracts and essential oil, and markers of hepatotoxicity were investigated.
Results: The data revealed that the n-hexane extract, effectively attenuated CCl4‑induced toxicity in both in vitro and in vivo models in current investigation.
Conclusion: As the oxidative stress markers were ameliorated, it might be concluded that anise seed possesses protective effects probably due to its antioxidant constituents.- انتشار مقاله: 06-12-1393
- نویسندگان: Akram Jamshidzadeh,Reza Heidari,Mojtaba Razmjou,Forouzan Karimi,Mahmood Reza Moein,Omid Farshad,Amin Reza Akbarizadeh,Mohammad Reza Houshangi Shayesteh
- مشاهده