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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Lung cancer,Survival,EGFR expression
- چکیده:
- چکیده انگلیسی: Background: EGFR over-expression plays a key role in the development and progression of lung cancer. However, its status as a prognostic biomarker for survival outcomes is unclear. Objectives: To evaluate the prognostic utility of serum EGFR mRNA expression in Non-Small cell lung cancer (NSCLC) for treatment response and survival. Methods: EGFR mRNA levels were determined in serum using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Based on ROC curve, a cut off value of 16.0-fold increase was selected to categorize patients into low EGFR (≤ 16.0) and high EGFR (> 16.0) groups. Results: A total of 350 subjects were included (78.3% males), with mean (± SD) age of 57.1 (± 11.2) years, and including 247 (70.6%) adenocarcinoma (ADC). Majority (73.1%) had metastatic (stage IV) disease. Patients had higher pre-treatment serum EGFR mRNA levels than controls [median fold-increase (min, max), 16.2 (1.9, 66.7). Serum EGFR mRNA levels significantly reduced in those who achieved objective response and disease control. Significantly longer OS and PFS was observed in subjects having baseline EGFR mRNA expression ≤ 16.0 fold- increase compared to those with > 16.0 fold- increase [median (95% CI) OS: 25.0 (14.9, NR) versus 7.7 (6.3, 8.9) months; HR (95% CI) 2.9 (2.3, 4.0), p < 0.001; and PFS: 9.9 (7.1, 11.5) versus 6.0 (4.1, 7.5) months; HR (95% CI) 1.8 (1.3, 2.4), p < 0.001]. Conclusion: Serum EGFR mRNA expression is a useful parameter for predicting treatment response and survival outcomes in NSCLC.
- انتشار مقاله: 08-02-1398
- نویسندگان: Anant Mohan,Ashraf Ansari,Mirza Masroor,Alpana Saxena,R M Pandey,Ashish Upadhyay,Kalpana Luthra,G C Khilnani,Deepali Jain,Rakesh Kumar,Randeep Guleria
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Chronic Myeloid,leukaemia,microRNA21,Prognosis biomarker
- چکیده:
- چکیده انگلیسی: Background: Chronic myeloid leukaemia (CML) is a myeloproliferative disorder categorized by malignant transformation of a single stem cell of hematopoietic cells. microRNAs (miRNAs) belong to transcription regulators in hematopoiesis and their altered expression associates with pathogenesis of CML. Aim: Current study aimed to access the miR-21 expression profile in CML patients and therapy response as well as its prognostic significance. Methods: 100 CML cases, 100 controls were included in study and miR-21 expression was analyzed. Overall 9.22 mean fold increased expression was observed in CML patients before treatment. Results: Patients with different CML phases such as chronic phase, accelerated phase and blast crisis showed 7.16, 10.30 and 13.20 fold increased expression respectively. Overall 3.57 mean fold expression was observed in imatinib treated patients suggested more than 5 fold decreased expression in CML patients. Prognostic significance was calculated and observed that miR-21 expression at 7.29 fold cutoff, 75% sensitivity and 50% specificity was observed (AUC=0.75, p<0.0001). Study observed miR-21 overexpression in CML patients as well as gradually increased expression with advancement of disease. Conclusion: miR-21 overexpression represented molecular prognostic marker and predictive tool enabling efficient monitoring of drug response and therapy outcomes in CML patients.
- انتشار مقاله: 21-12-1397
- نویسندگان: Masroor Ali Beg Mirza,Sameer Ahmad Guru,Saleh Mohammed Abdullah,Aliya Rizvi,Alpana Saxena
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Prognosis,Lung adenocarcinoma,EGFR1 (-191C/A) genotype,overall and progression free survival
- چکیده:
- چکیده انگلیسی: Background: The epidermal growth factor receptor 1 (EGFR1) plays a significant role in cell proliferation and
development. Its regulation in humans is very critical and incompletely understood in Non small cell lung cancer
(NSCLC). Methods: 100 newly diagnosed NSCLC (lung adenocarcinoma) patients and 100 healthy controls were
included and allele specific (AS) polymerase chain reaction (PCR) was used to genotype and expression was analyzed
by quantitative real time PCR. Overall survival of patients was analyzed by Kaplan-Meier method and for prognostic
significance ROC curve was plotted. Results: A statistically significant difference (p<0.0001) in CC, AA and CA
genotypes distribution among patients and healthy controls was observed. Compared to the CC genotype as reference,
OR was 30.40 (95%CI 1.75- 524.9, p=0.0002) and 3.97 (95%CI 1.49-10.52, p=0.003) for the homozygous AA and
heterozygous CA genotypes respectively. Kaplan-Meier survival analysis was also performed to analyze the relationship
of EGFR1 (-191C/A) genotypes with progression free median survival of NSCLC patients and the difference was
found to be significantly (p=0.0002) associated with different genotypes. In the ROC curve with respect to TNM stage
at optimal cut-off value of 9.88 fold increase in EGFR1 mRNA expression, sensitivity and specificity were 92.9%,
83.3% respectively (AUC=0.95, p<0.0001). ROC curve w.r.t. distant metastases at optimal cut-off value of 13.5 fold
change EGFR1 mRNA expression, sensitivity and specificity were 68.2%, 71.4% respectively (AUC=0.81, p<0.0001).
In ROC curve w.r.t to presence/ absence of pleural effusion at optimal cut-off value of 14.8 fold change EGFR1
mRNA expression sensitivity and specificity were 66.7%, 68.2% respectively (AUC=0.71, p=0.009). Conclusions:
Study concluded EGFR1 promoter polymorphism could be a risk factor associated with disease and may be used as
prognostic marker for patients’ survival and predictor for disease worseness.- انتشار مقاله: 22-05-1397
- نویسندگان: Mirza Masroor Ali Beg,Samer Riyadh Fahdil,Prasant Yadav,Kamla Kant Shukla,Anant Mohan,Alpana Saxena
- مشاهده