در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: HTLV-1,luciferase,Reporter gene,Biological Assay,Drug screening,Hut102
- چکیده:
- چکیده انگلیسی: Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously.
Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids.The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-1 was assayed by this system and was compared to the results obtained by other methods.
Results: HTLV-1 reporter cell line was found to produce high level of luciferase when co-cultured with MT-2 and Hut-102 cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-1 can reduce luciferase expression of the cell when co-cultured with MT-2 and Hut-102 comparable to the ELISA (R=0.932, P-value =0.002). In addition, the results revealed the superiority of the present system over the molecular methods.
Conclusion: The results demonstrated that the biological assay system is a beneficial tool for the medium-throughput anti-HTLV-1 drug screening and inhibitory effect.- انتشار مقاله: 04-07-1396
- نویسندگان: Mojtaba Fattahi Abdizadeh,Manoochehr Makvandi,Alireza Samarbafzadeh,Kayhan Azadmanesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Interleukin-6,Riboflavin,Brain-derived neurotrophic -factor,Experimental autoimmune -encephalomyelitis,Interleukin-17A,Motor disability
- چکیده:
- چکیده انگلیسی: Objective(s): In the present study, C57BL/6 female mice (n=56) were used to explore the neuroprotective effects of riboflavin in motor disability of experimental autoimmune encephalomyelitis (EAE) as a model of multiple sclerosis. Materials and Methods: The animals were assigned into 7 groups: sham-operated 1 (SO1), healthy mice receiving PBS (phosphate buffer saline); sham-operated 2 (SO2), healthy mice receiving PBS and riboflavin; sham treatment 1 (ST1), EAE mice receiving water; sham treatment 2 (ST2), EAE mice receiving sodium acetate buffer; treatment 1 (T1), EAE mice receiving interferon beta-1a (INFβ-1a); treatment 2 (T2), EAE mice receiving riboflavin; treatment 3 (T3), EAE mice receiving INFβ-1a and riboflavin. After EAE induction, scoring was performed based on clinical signs. Upon detecting score 0.5, riboflavin at 10 mg/kg of body weight and/or INFβ-1a at 150 IU/g of body weight administration was started for two weeks. The brain and spinal cord levels of brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), and interleukin-17A (IL-17A) were studied using real-time PCR and ELISA methods. Results: BDNF expression and protein levels were increased in the brain and spinal cord of the T3 group compared with the other groups (P<0.01). IL-6 and IL-17A expressions were increased in the brains of the T3 and T1 groups, respectively, compared to the other groups (P<0.01). The daily clinical score was reduced significantly by riboflavin in both effector and chronic phases of the disease compared with that of the controls (P<0.05). Conclusion: Our findings showed that riboflavin is capable of suppressing the neurological disability mediated by BDNF and IL-6.
- انتشار مقاله: 05-02-1395
- نویسندگان: Mahshid Naghashpour,Reza Amani,Alireza Sarkaki,Ata Ghadiri,Alireza Samarbafzadeh,Sima Jafarirad,Amal Saki Malehi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Polymerase Chain Reaction,Ductal carcinoma,Human Cytomegalovirus
- چکیده:
- چکیده انگلیسی: Breast cancer is the most common cause of death among women worldwide. Although there are many known risk
factors in breast cancer development, infectious diseases have appeared as one of the important key to contribute to
carcinogenesis formation. The effects of Human Cytomegalovirus (HCMV) on women with breast cancer has been
recently studied and reported. To contribute to this research trend, this study was conducted to evaluate the association
between HCMV and the women with breast cancer. Objective: This experiment aimed to evaluate HCMV DNA in women
with breast cancer in Ahvaz city, Iran. Materials and Methods: A total of 37 formalin fixed paraffin embedded tissues of
the patients with ductal breast carcinoma and 35 paraffin embedded tissues of the patients with fibro adenoma as control
group were collected. The deparaffinization of all the samples were carried out and the DNA was extracted. Initially,
the PCR test was carried out to detect beta –globulin DNA as an internal control. For those samples positive for beta
–globulin DNA, Polymerase Chain reaction (PCR) was used to detect HCMV for the tests and control samples. Results:
Among 37 ductal breast carcinoma, 20 (54.04%) cases were proved positive for HCMV DNA by PCR. While among
the 35 control group (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028). The prevalences of
HCMV DNA among the age groups 30-39, 40-49 and >50 years were 7 (72.22%), 9 (69.23%), 4 (57.14%), respectively
(P=0.066). A high frequency of HCMV DNA was detected in tumor grade III, 13/18 (58.33%) compared with tumor
grade II, 7/19 (36.84%) (p=0.044). A high frequency of 16/24 (66.66%) of HCMV DNA was found in invasive ductal
breast cancer compared with 4/13 (30.76%) HCMV DNA in situ (P<0.028). Conclusion: A high prevalence of 54.05%
HCMV was found among the patients with ductal carcinoma. The percentages of the high prevalence of HCMV among
age group (40-49) years, tumors grades, and invasive stage were (69.23%), (58.33%), (66.66%), respectively. Further
study of HCMV in the latency phase in patients with ductal carcinoma would be necessary to extend our knowledge.- انتشار مقاله: 22-06-1397
- نویسندگان: Peyman Sepahvand,Manoochehr Makvandi,Alireza Samarbafzadeh,Abdulhasan Talaei-Zadeh,Nastaran Ranjbari,Nilofar Nisi,Azarakhsh Azaran,Shahram Jalilian,Roya Pirmoradi,Kimia Makvandi,Kambiz Ahmadi Angali
- مشاهده