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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Mice,Cisplatin,Nephrotoxicity,Sumatriptan
- چکیده:
- چکیده انگلیسی: Objective(s): Cisplatin (Cis) is an anticancer compound, which is used for the treatment of various cancers. Sumatriptan (Suma) is a selective agonist of 5-hydroxytryptamine 1B/1D (5HT1B/1D) receptor, which is prescribed for the management of migraine. It is well-established that Suma has anti-inflammatory and antioxidant properties. We have explored the protective effects of Suma in the mitigation of Cis-induced nephrotoxicity.
Materials and Methods: The mice received a single IP injection of Cis (20 mg/kg) on the first day of the experiment. Suma treatment (0.1 and 0.3 mg/kg/day, IP) was started on day 1 and continued for 3 consecutive days.
Results: Creatinine (Cr), blood urea nitrogen (BUN) and malondialdehyde (MDA) levels were elevated and glutathione peroxidase (GPx) as well as superoxide dismutase (SOD) activities were decreased in Cis-treated mice. Suma (more potently 0.3 mg/kg) reduced Cr, BUN and MDA levels and increased SOD and GPx levels. Suma also reduced the acute renal injury (tubular degeneration, tubular cells vacuolation, tubular necrosis and cast), which corresponded to kidney damage in Cis-treated mice.
Conclusion: These findings demonstrate that Suma mitigates Cis-induced renal injury by inhibition of oxidative stress and enhancing the antioxidant enzymes activities.- انتشار مقاله: 01-05-1397
- نویسندگان: Gholamreza Bazmandegan,Morteza Amirteimoury,Ayat Kaeidi,Ali Shamsizadeh,Morteza Khademalhosseini,Mohammad Hadi Nematollahi,Mahsa Hasanipoor,Iman Fatemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Memory,Alzheimer’s disease,BDNF,AMPA receptors,CX691
- چکیده:
- چکیده انگلیسی: Objective(s): Growing evidence suggests that dysfunction of the glutamatergic system and α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptors are involved in pathology of Alzheimer’s disease (AD). Because AMPA receptors play a key role in plasticity synaptic regulation, positive modulation of these receptors may rescue the cognitive deficits in the AD. The aim of this study was to explore the effect of CX691, a specific positive allosteric modulator of the AMPA-type glutamate receptors (Ampakine), on spatial learning and memory in a rat model of AD.
Materials and Methods: For induction of AD, amyloid-beta 1-42 (Aβ1-42) was microinjected into the hippocampus of male Wistar rats (250-300 g). The Morris water maze (MWM) test was used to evaluate the effect of CX691 (0.03 and 0.3 mg/kg, twice a day for 10 days, orally) on spatial learning and memory of rats. In order to evaluate the protein expression of brain-derived neurotrophic factor (BDNF) in hippocampus tissue, ELISA test was used.
Results: The obtained data showed that treatment with CX691 (0.3 mg/kg) improves the impairment of spatial learning and memory in AD rats. Also, treatment with CX691 (0.3 mg/kg), increased the BDNF protein level in hippocampus tissue of AD rats compared to non-treated animals.
Conclusion: The CX691 can improve the BDNF protein expression as well as spatial performance of learning and memory in AD rats.- انتشار مقاله: 02-10-1396
- نویسندگان: Nazanin Mozafari,Ali Shamsizadeh,Iman Fatemi,Mohammad Allahtavakoli,Amir Moghadam-Ahmadi,Elham Kaviani,Ayat Kaeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Metformin,aging,Mouse,D-galactose
- چکیده:
- چکیده انگلیسی: Objective(s): Metformin (Met), an antidiabetic biguanide, reduces hyperglycemia via improving glucose utilization and reducing the gluconeogenesis. Met has been shown to exert neuroprotective, antioxidant and anti-inflammatory properties. The present study investigated the possible effect of Met on the D-galactose (D-gal)-induced aging in mice.
Materials and Methods: Met (1 and 10 mg/kg/p.o.), was administrated daily in D-gal-received (500 mg/kg/p.o.) mice model of aging for six weeks. Anxiety-like behavior, cognitive function, and physical power were evaluated by the elevated plus-maze, novel object recognition task (NORT), and forced swimming capacity test, respectively. The brains were analyzed for the level of superoxide dismutase (SOD) and brain-derived neurotrophic factor (BDNF).
Results: Met decreased the anxiety-like behavior in D-gal-treated mice. Also, Met treated mice showed significantly improved learning and memory ability in NORT compared to the D-gal-treated mice. Furthermore, Met increased the physical power as well as the activity of SOD and BDNF level in D-gal-treated mice.
Conclusion: Our results suggest that the use of Met can be an effective strategy for prevention and treatment of D-gal-induced aging in animal models. This effect seems to be mediated by attenuation of oxidative stress and enhancement of the neurotrophic factors.- انتشار مقاله: 25-03-1396
- نویسندگان: Iman Fatemi,Amin Khaluoi,Ayat Kaeidi,Ali Shamsizadeh,Sara Heydari,Mohammad Allahtavakoli
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Inflammation,TLR4,TLR2,Cerebral ischemia,TRPV1
- چکیده:
- چکیده انگلیسی: Objective(s): Stroke is known as a main cause of mortality and prolonged disability in adults. Both transient receptor potential V1 (TRPV1) channels and toll-like receptors (TLRs) are involved in mediating the inflammatory responses. In the present study, the effects of TRPV1 receptor activation and blockade on stroke outcome and gene expression of TLR2 and TLR4 were assessed following permanent middle cerebral artery occlusion in rats
Materials and Methods: Eighty male Wistar rats were divided into four groups as follows: sham, vehicle, AMG9810 (TRPV1 antagonist) -treated and capsaicin (TRPV1 agonist) -treated. For Stroke induction, the middle cerebral artery was permanently occluded and then behavioral functions were evaluated 1, 3 and 7 days after stroke.
Results: TRPV1 antagonism significantly reduced the infarct volume compared to the stroke group. Also, neurological deficits were decreased by AMG9810 seven days after cerebral ischemia. In the ledged beam-walking test, the slip ratio was enhanced following ischemia. AMG9810 decreased this index in stroke animals. However, capsaicin improved the ratio 3 and 7 days after cerebral ischemia. Compared to the sham group, the mRNA expression of TLR2 and TLR4 was significantly increased in the stroke rats. AMG9810 Administration significantly reduced the mRNA expression of TLR2 and TLR4. However, capsaicin did not significantly affect the gene expression of TLR2 and TLR4.
Conclusion: Our results demonstrated that TRPV1 antagonism by AMG9810 attenuates behavioral function and mRNA expression of TLR2 and TLR4. Thus, it might be useful to shed light on future therapeutic strategies for the treatment of ischemic stroke.- انتشار مقاله: 14-05-1396
- نویسندگان: Elham Hakimizadeh,Ali Shamsizadeh,Ali Roohbakhsh,Mohammad Kazemi Arababadi,Mohammad Reza Hajizadeh,Mehdi Shariati,Iman Fatemi,Amir Moghadam-ahmadi,Gholamreza Bazmandegan,Hossein Rezazadeh,Mohammad Allahtavakoli
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Neurodegenerative disease,plants,Flavonoids,medicinal,Achillea millefolium
- چکیده:
- چکیده انگلیسی: The Achillea millefolium L. (Yarrow) is a common herb which is widely being used, worldwide. Achillea is being used for treatment of many disorders since centuries. It is considered safe for supplemental use and flavonoids such as kaempferol, luteolin and apigenin are of main constituents present in Achillea. Most of both antioxidant and anti-inflammatory properties of this herb have been attributed to its flavonoid content. Oxidative and inflammatory processes play important roles in pathogenesis of neurodegenerative diseases. Present review was aimed to review the latest literature evidences regarding application of Achillea and/or its three main flavonoid constituents on epilepsy, Alzheimer's disease, multiple sclerosis, Parkinson's disease and stroke.
- انتشار مقاله: 13-03-1396
- نویسندگان: Fatemeh Ayoobi,Ali Shamsizadeh,Iman Fatemi,Alireza Vakilian,Mohammad Allahtavakoli,Gholamhossein Hassanshahi,Amir Moghadam-Ahmadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Formalin test,Orexin receptors,Rostral ventromedial medulla,Stress induced analgesia
- چکیده:
- چکیده انگلیسی: Objective(s): Intracerebroventricular injection of orexin-A (hypocretin-1) antagonist has been shown to inhibit stress-induced analgesia. However the locations of central sites that may mediate these effects have not been totally demonstrated. This study was performed to investigate the role of rostral ventromedial medulla (RVM) orexin receptor 1 in stress-induced analgesia (SIA).
Materials and Methods: Forced swim stress in water was employed to adult male rats (200-250 g). Nociceptive responses were measured by formalin test (50 µl injection of formalin 2% subcutaneously into hind paw) and, pain related behaviors were monitored for 90 min following intra-microinjection of SB-334867 (orexin receptor 1 antagonist) into RVM.
Results: Exposure to swimming stress test after administration of SB-334867 into RVM significantly reduces the formalin-induced nociceptive behaviors in phase1, interphase, and phase 2 in rats.
Conclusion: The result demonstrated the involvement of OXR1 in antinociceptive behaviors induced by swim stress in RVM.- انتشار مقاله: 01-03-1395
- نویسندگان: Neda Soliemani,Alireza Moslem,Ali Shamsizadeh,Hassan Azhdari-Zarmehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: anxiety,Rats,Anandamide,CA1 region,Fatty-acid amide hydrolase,TRPV Cation Channels
- چکیده:
- چکیده انگلیسی: Objective(s) Fatty acid is amide hydrolase which reduce endogenous anandamide. Transient receptor potential vanilloid-1 (TRPV1) channels have been reported to have a role in the modulation of anxiety-like behaviors in rodents. In the present study, the effects of either endocannabinoid system or TRPV1 channels and their possible interaction on anxiety-like behaviors of the rats were explored. Materials and Methods Elevated plus-maze test of anxiety was used to induce anxiety. Capsaicin and AMG 9810 as TRPV1 agonist and antagonist respectively were injected into the dorsal hippocampus. URB 597 as selective FAAH inhibitor and AM 251 as CB1 receptor selective antagonist were also injected into the dorsal hippocampus. The effect of AMG 9810 on the response of URB 597 was also examined. Results Intra-CA1 injection of URB 597 (0.001, 0.01 and 0.1 µg/rat) and AMG 9810 (0.003, 0.03 and 0.3 µg/rat) produced anxiolytic-like effects. Intra-CA1 infusion of capsaicin (0.003, 0.03 and 0.3 µg/rat) increased the anxiety-related behaviors and AM 251 (0.001, 0.01 and 0.1 µg/rat) did not significantly change the animals behavior. AMG 9810 at the dose of 0.003 µg/rat did not change the anxiolytic-like effect of URB 597. Conclusion The results of the present study demonstrated that both endocannabinoid system and TRPV1 receptors may affect anxiety-like behaviors. In addition, it seems that TRPV1 receptors are not involved in the effects of anandamide on anxiety-related behaviors in the CA1 region.
- انتشار مقاله: 25-06-1394
- نویسندگان: Elham Hakimizadeh,Shahrbanoo Oryan,Akbar Hajizadeh Moghaddam,Ali Shamsizadeh,Ali Roohbakhsh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Addiction,Cerebral ischemia,opiates,Embolic stroke,Morphine withdrawal syndrome
- چکیده:
- چکیده انگلیسی: Objective(s) Opioid abuse is still remained a major mental health problem, a criminal legal issue and may cause ischemic brain changes including stroke and brain edema. In the present study, we investigated whether spontaneously withdrawal syndrome might affect stroke outcomes. Materials and Methods Addiction was induced by progressive incremental doses of morphine over 7 days. Behavioral signs of withdrawal were observed 24, 48 and 72 hr after morphine deprivation and total withdrawal score was determined. Cerebral ischemia was induced 18-22 hr after the last morphine injection by placing a natural clot into the middle cerebral artery (MCA). Neurological deficits were evaluated at 2, 24 and 48 hr after ischemia induction, and infarct size and brain edema were determined at 48 hr after stroke. Results Morphine withdrawal animals showed a significant increase in total withdrawal score and decrease of weight gain during the 72 hr after the last morphine injection. Compared to the addicted and control animals, infarct volume and brain edema were significantly increased in the morphine deprived animals (P< 0.05) at 48 hr after cerebral ischemia. Also, neurological deficits were higher in the morphine-withdrawn rats at 48 hr after stroke (P< 0.05). Conclusion Our data indicates that spontaneous withdrawal syndrome may worsen stroke outcomes. Further investigations are necessary to elucidate mechanisms of opiate withdrawal syndrome on stroke.
- انتشار مقاله: 28-06-1394
- نویسندگان: Mohammad Allahtavakoli,Ruhollah Moloudi,Mohammad Ebrahim Rezvani,Ali Shamsizadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: type 2 diabetes,Polymorphism,Interferon-gamma,Interleukin-4
- چکیده:
- چکیده انگلیسی: Objective(s) Although, type 2 diabetes is the most frequent type of diabetes, its main cause is yet to be clarified. Several environmental and genetic parameters are believed to be involved in diabetes. It has also been established that cytokines play key roles in pathogenesis of diabetes. Expression of cytokines is different from person to person and in different societies. Several studies showed that polymorphisms of +874 of interferon-gamma (IFN-y) and -590 of interleukin-4 (IL-4) are associated with the regulation of expression of these genes. This study was aimed to find polymorphisms of these regions in type 2 diabetes patients. Materials and Methods In this experimental study peripheral blood samples were collected from 160 type 2 diabetic patients and 160 healthy controls. DNA was extracted by salting out method. Polymorphisms of +874 of IFN-y and -590 of IL-4 were analyzed by ARMS-PCR and RFLP-PCR. Results Our findings indicated that TT genotype of IFN-y was increased in type 2 diabetic patients compared to the control but difference was not significant. Our results didn’t show any significant difference between IL-4 genotype in diabetic and healthy controls either. Conclusion Our results suggested that TT genotype of IFN-y can be associated with diabetes. This association can be described by the fact that over expression of IFN-y shifts immune system to Th1; therefore, pancreatic cells can be miscarried by immune cells.
- انتشار مقاله: 08-07-1394
- نویسندگان: 2Mohammad Kazemi Arababadi,Ali Akbar Pourfathollah,Saeed Daneshmandi,Gholamhossein Hassanshahi,Ebrahim Rezazadeh Zrandi,Ali Shamsizadeh,Majid Asiabanha Rezaei,Somayeh Eigder
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Prediction,Colorimetric algorithm,Beer’s law,Dye concentration,Absorbance spectra
- چکیده:
- چکیده انگلیسی: This publication uses a colorimetric method based on the absorbance spectra of dye solutions to improve on Beer’s law when calculating the dye concentrations of a three-component mixture. The performance of the new method is compared with that of Beer’s law evaluated at three wavelengths, and Beer’s law evaluated at 16 wavelengths. Colorimetric method gives the best prediction of dye concentrations: the average relative errors in its predictions of the blue, red, and yellow concentrations are 55.64%, 12.3% and 14.84% respectively for new test solutions. Its average ternary relative error is 11.68% which is comparable to 14.31% for Beer’s law at three wavelengths.
- انتشار مقاله: 11-02-1389
- نویسندگان: Ali Shams-Nateri,Ali Shams-Nateri,Ali Shams-Nateri
- مشاهده