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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Horticultural Science and Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Molecular markers,Cucumis melo,Fusarium wilt,Landrace
- چکیده:
- چکیده انگلیسی: Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers resistance to race 1 of Fusarium in muskmelon, to screen muskmelon landraces in Khorasane-e-Razavi, Iran. After validation of the markers on a differential set of resistant and susceptible lines, we identified STS312 marker as the polymorphic and easy-to-score marker. Then we used STS312 to genotype plants from five different landraces. Our results suggest that resistance allele of Fom-2 gene is present in two landraces: Eyvankey and Mashhadi. These landraces can be used by muskmelon breeders to enhance resistance to Fusarium wilt in muskmelon.
- انتشار مقاله: 01-01-1399
- نویسندگان: Atefeh Gholizadegan,َAlireza Seifi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Horticultural Science and Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cell viability,calli,packed cell volume,phytohormones,triphenyltetrazolium chloride
- چکیده:
- چکیده انگلیسی: To establish a dynamic and fine suspension culture, four different methods of tomato cell suspension culture were compared. Hypocotyl explants of the tomato cultivar Jina were used for callus induction on Murashige and Skoog (MS) Medium supplemented with three different phytohormone combinations. Then, one gram of each type of calli was transferred to 50 mL of liquid MS medium with four combinations of auxins and cytokinins to produce cell suspensions. The growth rate, judged by cell turbidity, cell fresh weight, and cell viability was evaluated. The best suspension culture was obtained by using friable calli formed on MS medium containing 1 mg L-1 NAA and 0.1 mg L-1 kinetin, transferred to the liquid MS supplemented with 2 mg L-1 NAA, 0.2 mg L-1 2, 4-D and 0.2 mg L-1 zeatin.
- انتشار مقاله: 20-02-1399
- نویسندگان: Danial Shokouhi,Abdolreza Bagheri,َAlireza Seifi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Epidemiology,Carbon monoxide Poisoning
- چکیده:
- چکیده انگلیسی: Background: Knowing the pattern of carbon monoxide (CO) poisoning in each region is vital for enhanced health planning. This study was designed to evaluate the epidemiologic pattern of unintentional acute CO poisoning in major cities of Fars province, southwest of Iran. Methods: This one-year cross-sectional study was carried out on unintentional CO poisoning incidents in Fars province, Iran, during the year 2011. The target population was people living in 7 major cities under supervision of Shiraz University of Medical Sciences including Shiraz, Eghlid, Neyriz, Khorambid, Marvdasht, Darab and Bavanat. Results: During 2011, 111 CO poisoning events occurred in the catchment area. These events involved 420 individuals (50.2% men) who were present during the poisoning event, of which 281 individuals with mean age of 27.8 ± 14.8 years were poisoned (46.5% men). The majority of CO poisoning events (77.3%) occurred in colder months of the year. Most events happened in urban areas (61.3%). The most common source of CO was water heater (27.5%) closely followed by gas stove (24.8%). The majority of poisoned patients were asleep during the event (150/281: 53.3%). The main causes of CO generation were inbound gas return (62.2%) and inappropriate ventilation (28.8%). The fatality rate of CO poisoning was significantly higher in men compared to women both in involved individuals and poisoned patients (P = 0.035, < 0.001; respectively). Moreover, poisoned victims who were asleep during the accident were more likely to die than those who were awake (14.3 vs. 3.7%, P < 0.001). Conclusion: Generally, the incidence and fatality rate of CO poisoning in the current study were comparable to those of the world statistics, but higher than in developed counties. Attention and emphasis on the safety of gas heaters, stoves, and other gas-powered appliances in residential places should be enforced.
- انتشار مقاله: 25-07-1394
- نویسندگان: Alireza Mirahmadizadeh,Hossain Faramarzi,Effat Hadizadeh,Mohsen Moghadami,Mozhgan Fardid,Ali Seifi
- مشاهده