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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Epithelial Ovarian Cancer,Human papillomavirus 16,Human Papillomavirus 18
- چکیده:
- چکیده انگلیسی: Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, some studies did not detect HPV DNA in ovarian carcinoma tissues. In this article, we investigated the potential role of high risk HPVs in the ovarian epithelial carcinoma. Methods: Fifty archived epithelial ovarian cancer paraffin blocks were collected. Then, 30 non-malignant ovarian blocks used as control. These samples were histopathologically were confirmed by a pathologist and the proper blocks for DNA extraction and PCR were sorted. PCR was conducted deploying highly specific primers for high-risk types of HPV (18 and 16) according to the instructions of manufacturer company. Results: High-risk oncogenic sequences were identified in 4 (5%) of the 80 studied samples. Of the 4 HPV positive cases, there was 1 case with normal tissue, 1 case of mucinous cyst adenocarcinoma, and 2 cases of serous cyst adenocarcinoma Conclusion: Surprisingly, our findings could not support any association between high-risk oncogenic human papilloma virus (18 and 16) and malignant ovarian epithelial cancer. Therefore, that HPV is highly unlikely to play any causal role in the pathogenesis of epithelial ovarian neoplasia.
- انتشار مقاله: 19-11-1389
- نویسندگان: Ghodsie Alavi,Nourieh Sharifi,Ali Sadeghian,Alireza Rezaei,Hossein Shidaee
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,HPV,Skin Tag
- چکیده:
- چکیده انگلیسی: Objective(s) Human papillomavirus (HPV) infections are related to the genesis of various benign lesions and some malignant tumors, but no clear relationship has been identified so far between the subtypes of HPV and skin tag. Materials and Methods The present case-control study was designed to detect the existence of low risk and high risk HPV types in lesions of 50 patients with skin tag (case group) and normal skin around the melanocytic nevus of 30 patients (control group), using PCR. Results All of the samples were negative for HPV subtypes, except two samples in control group which were positive for high risk HPV. There was no significant relationship between the HPV subtypes and skin tag. Conclusion There is no association between skin tag and low risk and high risk human papillomaviruses.
- انتشار مقاله: 25-06-1394
- نویسندگان: Fakhrozaman Pezeshkpoor,Amir Hossein Jafarian,Kiarash Ghazvini,Mohammad Javad Yazdanpanah,Ali Sadeghian,Habiballah Esmaili,Maryam Karrabi,Fatemeh Rohani,Bahareh Joushan,Mohammad Shirdel
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Molecular cloning,Mycobacterium Tuberculosis,Gene tb'0.4,Protein TB'0.4
- چکیده:
- چکیده انگلیسی: Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. The aim of this study was to clone and express the TB'0.4 protein in Escherichia coli expression system. Materials and Methods DNA was extracted from Mycobacterium tuberculosis H37Rv. Gene specific primers were designed using Gene Runner software according to sanger sequence database. Gene tb'0.4 fragment was amplified by PCR method and purified tb'0.4 gene was cloned into pET 102/D vector. Plasmid containing pET102/D-10.4 was transformed into competence E. coli TOP'0. A positive transformant was chosen and plasmids DNA was isolated and subsequently transformed into competence E. coli BL2'(DE3). The bacterium was induced by IPTG and its lysates were loaded directly onto SDS-PAGE. Purified recombinant protein was achieved using metal affinity chromatography (Ni-nitrilotriacetic acid). Results TB'0.4 molecule was successfully cloned, expressed, and purified. An approximately 26.4 kDa exogenous protein was observed on the SDS-PAGE. The recombinant protein was confirmed by DNA sequencing of correct insert. Conclusion The success of expressing the TB'0.4 protein could serve as a basis for further studies on the usefulness of the gene and its expression product in the development of subunit vaccine and diagnostic method.
- انتشار مقاله: 07-07-1394
- نویسندگان: Aida Gholoobi,Mojtaba Sankian,Reza Zarif,Zahra Farshadzadeh,Forugh Youssefi,Ali Sadeghian,Mohammad Derakhshan,Abdol-Reza Varasteh
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Infant,barrier,Benefit,Kangaroo mother care,Premature
- چکیده:
- چکیده انگلیسی: Background
Kangaroo Mother Care (KMC) is an easy and emotional care that can reduce the mortality and morbidity in premature infants, but often the relationship between mother and her newborn is delayed due to some barriers. The aim of this study was to investigate perceived benefits and barriers of mothers in this regard.
Materials and Methods: In this descriptive cross-sectional study, 121 mothers with premature infants admitted to the Neonatal Intensive Care Unit (NICU) of Yazd Shahid Sadoughi Hospital, Iran, studied using convenience sampling method in 2018. Data gathering accomplished by interviewing mothers using researcher-made questionnaire included baseline characteristics of parents and their infant and items measuring perceived benefits and barriers of mothers to KMC. The data were analyzed using SPSS software version 22.0
Results: Strengthening of the emotional relationship between mother and infant and a greater sense of confidence were the main perceived benefits of mothers. Not being in good physical and mental condition, lack of relevant knowledge and fear of performing KMC were the most important perceived barriers of mothers to KMC. The perceived benefits differed only in mother's ethnicity and father's occupation (P<0.05). The perceived barriers did not differ by any of demographic variables of parents and infants (P>0.05).
Conclusion
According to the results though the high perceived benefits of mothers in performing KMC, it seems that planning for improving mothers mental condition, providing mothers with necessary knowledge and reducing fear of caring can effectively promote doing KMC by mothers.- انتشار مقاله: 10-07-1397
- نویسندگان: Masoumeh Abbasi- Shavazi,Sedigheh Safari Hajataghaiee,Hosseinali Sadeghian,Mahmood Noori Shadkam,Mohsen Askarishahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Staphylococcus aureus,Pseudomonas aeruginosa,Pomegranate
- چکیده:
- چکیده انگلیسی: Objective: Punica granatum, commonly known as pomegranate, has emerged as a medicinal plant with potential antimicrobial activity. The present study was planned to evaluate this activity against both Gram positive Staphylococcus aureus (S. aureus) and negative Pseudomonas aeruginosa (P. aeruginosa) bacteria as well as against pathogenic yeast, Candida albicans (C. albicans). Material and Methods: The aqueous and methanolic extracts of pomegranate fruit skin were prepared using a Soxhalet apparatus. Antimicrobial effect of the extracts was studied and compared with commercial antibiotics using three different methods; agar dilution, cylinder plate, and disk inhibition zone techniques. Results: Both extracts showed good antibacterial activity against S. aureus and P. aeruginosa. Also the methanolic extract presented strong antifungal effect on C. albicans. The antimicrobial activities against S. aureus, P. aeruginosa and C. albicans were comparable with those of cloxacillin, gentamycin and clotrimazole, respectively. The methanolic extract was found to be more effective than aqueous one against all the tested microorganisms. Conclusion: The extracts from pomegranate fruit skin possess strong antimicrobial activity against the tested microorganisms. Therefore this plant could be an important source of new antimicrobial compounds to treat bacterial and fungal infections.
- انتشار مقاله: 02-11-1391
- نویسندگان: Ali Sadeghian,Ahmad Ghorbani,Ahmad Mohamadi-Nejad,Hassan Rakhshandeh
- مشاهده