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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: optimization,Inactivated influenza vaccine,incubation temperature
- چکیده:
- چکیده انگلیسی: There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-day old specific pathogen free (SPF) ECEs in the 0.1 mL per ECE rate and incubated in 32, 33, 34, 35, 36, 37.5, 38, 39 ˚C for 72 hr in 65% humidity. Early death embryos in first 24 hr were removed. Amnio-allantoic fluid was withdrawn into the measuring cylinder, and tested for hemagglutination (HA) activity and egg infective dose 50 (EID50). The utilizable ECEs and amnio-allantoic fluid volume was significantly increased in 35 ˚C, (p < 0.05). Significant difference in HA and EID50 titers, were seen only in 39 ˚C group. Therefore, 35°C is an optimum temperature for incubation of inoculated ECEs.
- انتشار مقاله: 23-05-1392
- نویسندگان: Iraj Khalili,Rahim Ghadimipour,Ali Ameghi,Saeed Sedigh-Eteghad
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Inactivation,Formalin,Influenza virus H9N2,Avian influenza vaccine
- چکیده:
- چکیده انگلیسی: Avian influenza is one of the most important diseases in avian industry, which also threats human population. Thus, vaccination is necessary for controlling this viral disease. In this study, killed vaccine of avian influenza subtype H9N2 and formalin solution (for virus inactivation) were used. It is necessary to study the effect of different factors such as formalin concentration, as well as incubation temperature and duration on inactivation process. For this purpose, after preparation and measurement of antibody titers of vaccinal strains of avian influenza, 27 experimental samples of H9N2 avian influenza virus were prepared at different formalin concentrations (0.1%, 0.05%, and 0.025%), at different incubation temperatures (4 °C, 25 °C, and 37 °C), and in different incubation durations (12, 18, and 24 h). In addition, three control samples were prepared at three different test temperatures without adding formalin. All the samples were evaluated by inactivation test, hemagglutination assay, and measurement of free formaldehyde. All the experiments were repeated within three consecutive periods. Considering the findings and destructive effects of long incubation durations at 37°C on antigens, the temperature of 25 °C was more suitable. Furthermore, the free formaldehyde amounts at different concentrations at 25 °C were slightly different in comparison with 37 °C. Therefore, formalin concentration of 0.1% at 25 °C completely inactivated the virus within 24 h and was proposed as the optimal condition.
- انتشار مقاله: 11-12-1394
- نویسندگان: Behrooz Raie Jadidi,Hamid Erfan-Niya,Ali Ameghi
- مشاهده