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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,MTT assay,Key words: Ginseng,MDA-231,Quantitative real time PCR
- چکیده:
- چکیده انگلیسی:
Objective: Breast cancer is global female health problem worldwide. Most of the currently used agents for breast cancer treatment have toxic side-effects. Ginseng root, an oriental medicine, has many health benefits and may exhibit direct anti-cancer properties. This study was performed to assess the effects of ginseng on breast cancer cell lines. Materials and Methods: Cytotoxicity of ginseng extract was measured by MTT assay after exposure of MDA-MB-231, MCF-10A and MCF-7 breast cancer cells to concentrations of 0.25, 0.5, 1, 1.5, 2 and 2.5 mg/well. Expression levels of p21WAF, p16INK4A, Bcl-2, Bax and P53 genes were analyzed by quantitative real time PCR. Results: The treatment resulted in inhibition of cell proliferation in a dose-and time-dependent manner. p53, p21WAF1and p16INK4A expression levels were up-regulated in ginseng treated MDA-MB-231 and MCF-7 cancer cells compared to untreated controls and in MCF-10A cells. The expression levels of Bcl2 in the MDA-MB-231 and MCF-7 cells were down-regulated. In contrast, that of Bax was significantly up-regulated. Conclusion: The results of this study revealed that ginseng may inhibit breast cancer cell growth by activation of the apoptotic pathway.- انتشار مقاله: 13-07-1395
- نویسندگان: Othman Al-Shabanah,Moureq Alotaibi,Salim Al Rejaie,Ali Alhoshani,Mashal Almutairi,Musaad Alshammari,Mohamed Hafez
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Real Time PCR,interleukin 10,small interference RNA,caspase-3,Caspase-9
- چکیده:
- چکیده انگلیسی: Background: Breast cancer is affected by the immune system in that different cytokines play roles in its initiation
and progression. Interleukin-10 (IL-10), an anti-inflammatory cytokine, is an immunosuppressive factor involved in
tumorigenesis. The present study was conducted to investigate the gene silencing effect of a small interference RNA
(siRNA) targeting IL-10 on the apoptotic pathway in breast cancer cell line. Methods: The siRNA targeting IL-10 and
a glyceraldehyde 3-phosphate dehydrogenase (GAPDH) clone were introduced into MDA-MB-231 cells. Real-time
PCR assays were used to determine IL-10 and GAPDH gene expression levels, in addition to those for protein kinase
B (AKT), phosphoinositide 3-kinase (PI3K), B-cell lymphoma 2 (Bcl2), caspase-3 and caspase-9 genes related to
apoptosis. Results: Inhibition of IL-10 by the siRNA accelerated apoptosis and was accompanied by significant
increase in caspase-3 and caspase-9 and a significant decrease in PI3K, AKT and Bcl2 expression levels compared to
the non-transfected case. Conclusions: In conclusion, the production of IL-10 may represent a new escape mechanism
by breast cancer cells to evade destruction by the immune system. IL-10 gene silencing causes down regulation of both
PI3K/AKT and Bcl2 gene expression and also increases the Bbc3, BAX caspase3, and caspase 3 cleavage expression
levels. IL–10 might represent a promising new target for therapeutic strategies.- انتشار مقاله: 10-07-1396
- نویسندگان: Moureq R Alotaibi,Zeinab K Hassan,Salim S Al-Rejaie,Musaad A Alshammari,Mashal M Almutairi,Ali R Alhoshani,Wael A Alanazi,Mohamed H Hafez,Othman A Al-Shabanah
- مشاهده