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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Copper nanoparticles,Hematological parameters,fish,Lethality
- چکیده:
- چکیده انگلیسی: Objective(s):
This study aimed to determine the possible toxicity of Cuo nanoparticles (NPs) on Oncorhynchus mykiss by evaluating hematological parameters.
Materials and Methods:
Fish were sampled and treated in 4 aquariums containing the concentration ranges of 1, 5, 20 and 100 ppm of CuO NPs. There was one control group (no CuO NPs) and three replicates. The physicochemical properties of water were as follows: the temperature was 22±2 Cº, oxygen saturation was 90.9±0.2%, pH was at 7±0.004 and the concentration of CaCO3 was 270.
Results:
No mortality was observed after 96 hours of exposure. The analysis of hematological parameters showed that CuO NPs affected the counts of white blood cells, lymphocytes, eosinophils, neutrophils, hematocrits, MCH, MCHC and MCV and did not have any effects on monocytes and hemoglobins.
Conclusion:
The data showed that the overall hardness (270 ppm) neutralized the lethal effect of copper on O. mykiss and no mortality was recorded.- انتشار مقاله: 06-08-1393
- نویسندگان: Mahboobe Khabbazi,Mohammad Harsij,Seyed Ali Akbar Hedayati,Hosna Gholipoor,Mohammad Hasan Gerami,Hamed Ghafari Farsani
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Copper nanoparticles,Hematological parameters,fish,Lethality
- چکیده:
- چکیده انگلیسی: Objective(s):
This study aimed to determine the possible toxicity of Cuo nanoparticles (NPs) on Oncorhynchus mykiss by evaluating hematological parameters.
Materials and Methods:
Fish were sampled and treated in 4 aquariums containing the concentration ranges of 1, 5, 20 and 100 ppm of CuO NPs. There was one control group (no CuO NPs) and three replicates. The physicochemical properties of water were as follows: the temperature was 22±2 Cº, oxygen saturation was 90.9±0.2%, pH was at 7±0.004 and the concentration of CaCO3 was 270.
Results:
No mortality was observed after 96 hours of exposure. The analysis of hematological parameters showed that CuO NPs affected the counts of white blood cells, lymphocytes, eosinophils, neutrophils, hematocrits, MCH, MCHC and MCV and did not have any effects on monocytes and hemoglobins.
Conclusion:
The data showed that the overall hardness (270 ppm) neutralized the lethal effect of copper on O. mykiss and no mortality was recorded.- انتشار مقاله: 06-08-1393
- نویسندگان: Mahboobe Khabbazi,Mohammad Harsij,Seyed Ali Akbar Hedayati,Hosna Gholipoor,Mohammad Hasan Gerami,Hamed Ghafari Farsani
- مشاهده
- جایگاه : پژوهشی
- مجله: International Aquatic Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Beluga,gonad development,Steroid Hormones,Gonadic stage,brackish water
- چکیده:
- چکیده انگلیسی: The experiment was carried out in one year on blood sexual hormones in 74 four-years old beluga sturgeon Huso huso held in 8 round cement tanks in brackish water with salinity of about 13.5 ppt. Fishes were biopsied at the beginning of the experiment in order to determine the sexuality and gonadic stage. Blood sampling was performed in caudal veins three times during experiment in fall, winter and spring seasons.Testosterone (T), Esteradiol (E2) and Progesterone (P) were measured by Radio Immune Assay (RIA) method. Test of profile was done in duplicate. Statistical results showed a significant elevation (p < /em> > 0.05) on measured factors in both sexes, especially in males.In males, testosterone (T) depends on gonadic stage had significant elevation; but in females, there was no significant increase (p < /em> > 0.05) in estradiol (E2) and progesterone (P) which probably resulted from their ages. In males, T level in stages II, II-III, III, IV, were 5.5, 4.4, 6.3, 2.6 ng/ml, respectively.The result indicated that the amount of T increased by development of gonads until III stage, then suddenly decreased in stage IV. Quantities of P and E2 were measured only in females.Amounts of P and E2 in stages of II, II-III, were 0.12, 0.13 and 1.455, 0.81 ng/ml, respectively, suggesting the increase of P and decrease of E2 were associated with gonad progress. According to the results, it was demonstrated that sexual hormones were affected by season, environmental conditions and gonadal stages.Therefore, inland underground brackish water as a new medium culture was effective in reduction maturity time in beluga.
- انتشار مقاله: 26-08-1399
- نویسندگان: Morteza Alizadeh,Ali Akbar Hedayati,Mahmoud Bahmani,Hamid Reza Jamalzadeh
- مشاهده