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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Screening,Colorectal cancer,Colonoscopy,Polyps,Stool DNA
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. Aim: to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening. Method: Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. Results: KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity. Conclusion: Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine.
- انتشار مقاله: 23-11-1397
- نویسندگان: Jacqueline Lima,Yolanda Teixeira,Célia Pimenta,Aledson Vitor Felipe,Tiago Donizetti Silva,Ermelindo Ermelindo Della Libera Junior,Sarhan Sydney Saad,Elisabeth Deak,Helena Murray,Nora Manoukian Forones
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,Biomarker,Lipidomic
- چکیده:
- چکیده انگلیسی: Backgrounds: Colorectal (CRC) is one of the main cause of cancer worldwide. The search for noninvasive markers
for diagnosis and monitoring as the use of analytical technologies such as mass spectrometry (MS), which allowed the
search for lipid metabolites as candidates for probable biomarkers are needed. Objective and Methods: The objective
was to establish the lipid profile of patients with locally advanced, unresectable or metastatic CRC. Peripheral blood
was collected from patients with CRC and controls with normal colonoscopy. After lipid extraction, the samples were
processed and analyzed in the MALDI TOF / TOF equipment. From the data matrix, the statistical analyzes were
performed by the principal component analysis methods and the least squares discriminant analysis. The importance of
the variable in the projection was used to identify the ions that had the greatest discriminatory effect between the groups.
Results: Eight lipids were identified as potential biomarkers and a multiple logistic regression model was proposed
to calculate the performance of the test where we observed values of AUC 0.87, sensitivity 88.33% and specificity
83.78% and for a validation test with 1,000 permutations a p glycerophospholipids and policetidis. The strength of the association between the peak intensities of these lipids and
the presence of CRC make these metabolites candidates for possible biomarkers. The sphingolipid (m / z = 742.98869)
could be a biomarker in monitoring patients with CRC. In the survival analysis, three lipids showed a prognostic value
for colorectal cancer, sphingolipid (m / z = 857.11525) and policetidis (m / z = 876.20796) and glycerophospholipid
(m / z = 1031.54773).- انتشار مقاله: 30-07-1396
- نویسندگان: Adiel Goes De Figueiredo Junior,Patrícia Valéria Pereira Serafim,Augusto Azzolini De Melo,Aledson Vitor Felipe,Edson Guimarães Lo Turco,Ismael Dale Cotrim Guerreiro Da Silva,Nora Manoukian Forones
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,Gastric cancer,RNA interference,ABCB1,epirubicin
- چکیده:
- چکیده انگلیسی: Background: Gastric cancer is one of the most common malignancies worldwide. Epirubicin (EPI) is used
extensively in the treatment of multiple cancers despite its tendency to induce multidrug resistance though overexpression
of the ABCB1 efflux pump. However, this overexpression can be disrupted using short interfering RNAs (siRNAs).
Objective and Methods: The aim of this study was to explore approaches to reverse EPI resistance and thus increase
the success of chemotherapy treatment in an EPI-resistant gastric cancer cell subline (AGS/EPI). Methods: The study
focused on effects of ABCB1 knockdown by siRNA technology using TaqMan gene expression assays with quantitative
real-time reverse-transcription PCR (qRT-PCR). MTT assays were performed to evaluate viability and prolifer in subline.
ABCB1 protein localization and EPI intracellular fluorescence intensity in AGS/EPI cells were detected by confocal
microscopy. Results: The siRNA efficiently downregulated ABCB1 mRNA in AGS/EPI cells. Thus MDR reversal
was clearly demonstrated in the AGS/EPI cells, offering the possibility of future in vitro chemoresistance assays for
the GC field. Conclusions: ABCB1 knockdown decreased EPI efflux and increased EPI sensitivity in AGS/EPI cells.
This result provides a novel strategy for targeted gene therapy to reverse EPI resistance in gastric cancer.- انتشار مقاله: 18-04-1396
- نویسندگان: Aledson Vitor Felipe,Juliana De Oliveira,Andrea Aparecida Moraes,Jerônimo Pereira De França,Tiago Donizetti Da Silva,Nora Manoukian Forones
- مشاهده