در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,immunohistochemistry,hypermethylation,DAPK1,MSP
- چکیده:
- چکیده انگلیسی: Aims: Death-associated protein kinase-1 (DAPK1) is a pro-apoptotic Ser/Thr kinase that participates in cell apoptosis
and tumor suppression. DAPK1 is frequently lost in many different tumor types including breast cancer. The aim of
this study was to evaluate the promoter methylation status of DAPK1 and a possible correlation with the expression
of DAPK1 and standard clinicopathological features in invasive ductal breast carcinoma patients (IDC). Methods:
Methylation Specific PCR (MSP) was carried out to investigate the promoter methylation status of DAPK1 from 128
breast cancer patients. The effect of promoter methylation on protein expression was evaluated by immunohistochemistry
(n=128) and western blotting (n=56). Results: We found significant difference in DAPK1 promoter methylation
frequency among breast tumors when compared with the corresponding normal tissues. Hypermethylation of DAPK1
is significantly correlated with the loss of DAPK1 protein expression (P < .001, rs= -0.361). The loss of DAPK1 protein
was significantly associated with estrogen receptor (ER) negativity (p= 0.003), triple negative breast cancer (TNB)
(p= 0.024) and advanced tumor stages (P = 0.001). Moreover, age at diagnosis (p= 0.041), tumor stage (p= 0.034), ER
negativity (p= 0.004) and TNB cancers (p=0.003) correlated significantly with the hypermethylation of the DAPK1
promoter. Coclusion: This study indicates that DAPK1 is methylated in IDC and promoter hypermethylation could be
attributed to silencing of DAPK1 gene expression in breast cancer. Thus, we consider DAPK1 inactivation by promoter
hypermethylation likely plays a role in the development and progression of breast cancer.- انتشار مقاله: 27-05-1397
- نویسندگان: Asia Asiaf,Shiekh Tanveer Ahmad,Ajaz Ahmad Malik,Shiekh Aejaz Aziz,Mohammad Afzal Zargar
- مشاهده