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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gallic acid,gallic acid nanocomposite,anti-cancer,pharmacokinetic,bioavailability
- چکیده:
- چکیده انگلیسی: Objective: In this study, we investigated the in vivo antitumor activity and pharmacokinetic characteristics of
encapsulated GA-NC (gallic acid nanocomposite) in normal and hepatocellular carcinoma (HCC)-induced rats.
Methods: Rats were distributed into 4 groups; negative control, HCC, gallic acid (GA), and GA-NC. Serum levels of
alpha-fetoprotein (AFP), endoglin (ENG), heat shock protein-70 (HSP-70), pro-caspase 3, lipocalin-2 (LCN-2) and
β-cell leukemia/lymphoma 2 (Bcl-2) were assayed by ELISA. The pharmacokinetic parameters for GA or GA-NC
were determined by means of non-compartmental approach based on the serum– concentration profiles of free GA
and GA-NC after oral administration. Also, histological procedures were used for examination of liver tissue sections.
Results: Anaplastic changes in liver tissues were observed in untreated HCC group, as well as a significant increase
in the serum AFP level. In addition, significant elevation in the serum ENG level as an angiogenic marker and the
serum levels of the apoptotic mediators; HSP-70, Bcl-2 and pro-caspase 3 beside significant amplification in the serum
inflammatory modulator, LCN-2 were recorded. Treatment with free GA or GA-NC markedly recovered the anaplastic
changes in the rat liver tissues. In addition, they restored serum levels of AFP, ENG, HSP-70, Bcl-2, pro-caspase-3,
and LCN-2. Pharmacokinetic analysis revealed that GA–NC displayed a characteristic sustained release profile with
4-fold increase in bioavailability in normal and HCC-induced rats. Conclusions: The results of this study suggest that
encapsulation of GA into PLGA-CS-PEG enhances its oral bioavailability and anti-cancer activity. GA-NC may be
a new therapeutic candidate for the mitigation of hepatocarcinogenesis.- انتشار مقاله: 22-11-1396
- نویسندگان: Hanaa Ahmed,Asmaa F Galal,Aziza B Shalby,Ahmed A Abd-Rabou,Fathy M Mehaya
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Apoptosis,Moringa Oleifera,PLGA-CS-PEG nanoparticles
- چکیده:
- چکیده انگلیسی:
Medicinal plants are important elements of indigenous medical system that have persisted in developing countries. Many of the botanical chemo-preventions currently used as potent anticancer agents. However, some important anticancer agents are still extracted from plants because they cannot be synthesized chemically on a commercial scale due to their complex structures that often contain several chiral centers. The aim of this study was to test different extracts from the Moringa oleifera leaves (ML), its PLGA-CS-PEG nanocomposites (MLn), as well as root core (Rc) and outer (Ro) parts for activity against hepatocarcinoma HepG2, breast MCF7, and colorectal HCT 116/ Caco-2 cells in vitro. Nano-composites were prepared and characterized. Then, the nanocomposites and the free counterparts were screened on different propagated cancer cell lines. The underlying cytotoxic impact was followed using apoptosis measurements. All extracts kill the different cancer cells with different ratios, but intriguingly, the root core extract could kill the majority of cancer cells (approximately 70-80%), while sparing normal BHK-21 cells with minimal inhibitory effect (approximately 30-40%). Apoptotic cell increment came to confirm the cytotoxic effects of these extracts on HCT 116 cells (Rc: 212% and Ro: 180%, respectively) and HepG2 cells (ML: 567.5% and MLn: 608%, respectively) compared to control (100%) mechanistically wise. Moringa oleifera nanocomposites may have potential for use as a natural source of anti-cancer compounds.- انتشار مقاله: 15-01-1396
- نویسندگان: Ahmed A Abd-Rabou,Aboelfetoh M Abdalla,Naglaa A Ali,Khairy MA Zoheir
- مشاهده