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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,systemic lupus erythematosus,Telomerase,Lymphocyte
- چکیده:
- چکیده انگلیسی: Background & Objective:
It is not clear whether activated lymphocytes of patients with systemic lupus erythematosus (SLE) are more proliferative or less apoptotic. We aimed to delineate potential differences between B and T cells of SLE patients compared to healthy controls regarding the telomerase activity and apoptosis status.
Methods:
In this cross-sectional case control study, Blood samples were taken from 10 SLE patients and 10 healthy controls. B and T cells were separated using magnetic cell sorting system. Telomeric repeat amplification protocol (TRAP) assay and real-time PCR were used to determine the telomerase activity and the expression of alternatively spliced variants.
Result:
Four patients under treatment showed significant telomerase activity in their T cells. Four of the newly diagnosed patients showed telomerase activity in their B cells (20% of all patients and 40% of new onset patients). There was no specific pattern of human telomerase reverse transcriptase variant expression within the patients’ lymphocytes. A significantly reduced expression of Bcl-2 was detected in B cells (P=0.018) and a trend toward lower Bcl-2 expression in T cells was seen in SLE patients compared to healthy controls.
Conclusion:
Although not definitive, our results may suggest that B cells may have more active roles during the earlier phases of the disease attack, while T cells take over when the disease reaches its chronic stages.- انتشار مقاله: 18-04-1398
- نویسندگان: Armin Attar,Mohsen Khosravi Maharlooei,Mohammad Nazarnia,Ahmad Hosseini,Zohre Bajalli,Yalda Sadat Moeini,Ahmad Monabati,Fatemeh Amirmoezi,Mansooreh Jaberipour,Mojtaba Habibagahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast,Genetic heterogeneity,Receptor,ErbB-2
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 28-09-1396
- نویسندگان: Akbar Safaei,Ahmad Monabati,Maral Mokhtari,Mehdi Montazer
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Urinary bladder,like carcinoma,Carcinoma in situ
- چکیده:
- چکیده انگلیسی: Lymphoepithelial - like carcinoma, is rarely recognized in the urinary bladder and less commonly occurs with papillary transitional cell carcinoma i.e. mixed pattern. Also, less uncommon is the occurrence of carcinoma in situ changes in the adjacent urothelium of these tumors. Here, a case of lymphoepithelial – like carcinoma and papillary transitional cell carcinoma associated with carcinoma in situ changes of urothelium of the urinary bladder has been reported the prognosis of this type of malignancy as well as its management will be discussed. Meanwhile, immunohistochemical stains have been carried out to differentiate it from lymphoma of the urinary bladder and the findings will be discussed.
- انتشار مقاله: 03-06-1396
- نویسندگان: Yahya Attaran,Simin Moghdam,Ahmad Monabati,Reza Sarkeshikian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: acute myeloid leukemia,Fms-Like Tyrosine Kinase 3,Karyotype,CD123
- چکیده:
- چکیده انگلیسی: Background and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may assist in prognosis and treatment of patients with AML.
Methods: This study was performed on 76 patients as new cases of AML. The correlation between CD123 immunohistochemical (IHC) expression and FLT3 gene mutations with each other as well as morphological, immunophenotypical and cytogenetic factors was studied.
Results: The results represented the CD123 IHC expression in 55.3% and FLT3 gene mutations in 28.9% of cases. We found that 81.3% of patients who had FLT3/ITD gene mutations revealed IHC of CD123 expression (P=0.019). The CD123 expression against FLT3 was also correlated with monocytic differentiation in bone marrow blasts (P=0.031). There were significant correlations between IHC expression of CD123 and FLT3/ITD mutations with a high percentage of aspirated bone marrow blasts (P=0.01 and P=0.006, respectively) as well as the lack of CD34 expression in bone marrow blasts (P=0.007 and P=0.021, respectively).
Conclusion: The CD123 IHC positive AMLs were correlated with certain pathologic features, some of which can be similar with correlations of background mutation of FLT3/ITD; According to the negative predictive value (NPV), 88.2% of CD123 IHC showed FLT3 gene mutation. In addition to its use in targeted therapy, it could be a marker to decide what molecular tests to use in the next steps.- انتشار مقاله: 29-03-1396
- نویسندگان: Freidoon Solhjoo,Akbar Safaie,Ahmad Monabati,Maral Mokhtari,Moeinadin Safavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Squamous Cell Carcinoma,Human papilloma virus,tongue
- چکیده:
- چکیده انگلیسی: Background and objective: Oral tongue Squamous Cell carcinoma (SCC) commonly involves males between the sixth to eighth decades of life. Major risk factors are tobacco usage and alcohol consumption. The increasing number of patients developing oral tongue cancer without these well-known risk factors suggests that a viral infection, such as Human Papillomavirus (HPV), may be responsible for this increase, by acting as an oncogenic agent. This study investigated the prevalence of HPV infection and its clinicopathologic significance in oral tongue SCCs.
Material and methods: Tissue blocks from a total of 50 cases (patients with oral tongue SCC) and 50 controls (palatine tonsillar tissues with benign diagnosis) were selected. DNA was extracted from tumoral and non-tumoral tissue blocks. Detection of common HPV DNA by nested Polymerase Chain Reaction (PCR), and high-risk genotypes, HPV 16 and HPV 18, by conventional PCR, was achieved and the results correlated with clinicopathological parameters.
Results: Of the 50 patients (18 males and 32 females with a mean age of 57.36±12.18 years, and age range of 27 to 86 years), 7 (14%) had HPV positive results. None of the control group subjects had HPV DNA positive results (P-value of 0.012). The HPV genotype 16/18 was not detected in positive cases. No statistically significant association was found between HPV status and gender, age, tumor grade, tumor stage or lymph node involvement.
Conclusion: Although there was a significantly higher prevalence of HPV in oral tongue SCC, its association with carcinogenesis in this area requires further studies.- انتشار مقاله: 24-03-1395
- نویسندگان: Mohamad Javad Ashraf,Shahla Hosseini,Ahmad Monabati,Behnaz Valibeigi,Bijan Khademi,Elham Abedi,Negar Azarpira
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Leukemia,Dendritic Cells
- چکیده:
- چکیده انگلیسی: Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematodermic myeloid malignancy that is known to be derived from plasmacytoid dendritic cells which are characterized by expression of CD4, CD56, and more specific markers such as CD123. Here, the authors present three cases of BPDCN diagnosed in the past two years and address different available diagnostic modalities such as morphology, immunohistochemistry, flow cytometry, and cytogenetics. Overall, we believe that although BPDCN is a rare diagnosis, it should not be left unchecked. Currently, available immunophenotyping markers are of great help, but the main clue to figure out the problem of BPDCN is clinicopathologic suspicion.
- انتشار مقاله: 01-10-1396
- نویسندگان: Akbar Safaei,Ahmad Monabati,Maral Mokhtari,Freidoon Solhjoo,Mehdi Montazer
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Thyroid cancer,Chronic Lymphocytic Leukemia,renal cell carcinoma,Basal cell carcinoma,Second primary cancer
- چکیده:
- چکیده انگلیسی: The standard therapy for thyroid cancer is total or near total thyroidectomy, followed by the administration of radioactive iodine for remnant ablation or residual disease. Patients with radioiodine therapy are predisposed to second malignant neoplasms in organs such as central nervous system (CNS), breast, prostate, kidney, bone marrow, salivary gland, and digestive tract. Exposure to carcinogen including occupational and therapy related hazard, aging and genetic susceptibility are other causes of second primary cancers. The second primary malignancies are not uncommon and, nowadays, the prevalence of it is mildly increasing due to the increasing survival of cancer patients and advances in early diagnosis and therapeutic modalities. Here, we present a fifty-one-year-old man with papillary thyroid carcinoma (PTC), who developed chronic lymphocytic leukemia (CLL), renal cell carcinoma (RCC), and basal cell carcinoma (BCC) in 15-20 years after radioactive iodine therapy. Second primary tumors are increasing and environmental, genetic susceptibility and increase in survival of cancer patients are the major risk factors.
- انتشار مقاله: 16-04-1396
- نویسندگان: Mehdi Dehghani,Saranaz Jangjoo,Ahmad Monabati,Dena Masoomi Bandari,Nasrin Namdari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Squamous Cell Carcinoma,Polymerase Chain Reaction,Human papilloma virus,Esophagus
- چکیده:
- چکیده انگلیسی: Background: Carcinomas of esophagus, mostly squamous cell carcinomas, occur throughout the world. There are a number of suspected genetic or environmental etiologies. Human papilloma virus (HPV) is said to be a major etiology in areas with high incidence of esophageal carcinoma, while it is hardly detectable in low incidence regions. This study was designed to evaluate the prevalence of HPV in esophageal squamous cell carcinoma (ESCC) cases diagnosed in Pathology Department, Medical School, Shiraz University of Medical Sciences.
Methods: DNA material for PCR amplification of HPV genome was extracted from formalin-fixed paraffin-embedded tissue blocks of 92 cases of ESCC, diagnosed during 20 years from 1982 to 2002. Polymerase chain reaction was performed for amplification and detection of common HPV and type specific HPV-16 and HPV-18 genomic sequences in the presence of positive control (HPV-18 and HPV positive biopsies of uterine exocervix) and additional internal controls i.e. beta-globin and cytotoxic T lymphocyte antigen 4 (CTLA4).
Result: Good amplification of positive control and internal controls was observed. However, no amplification of HPV genome was observed.
Conclusion: There is no association between HPV infection and the development of esophageal squamous cell carcinoma in the cases evaluated.- انتشار مقاله: 11-09-1392
- نویسندگان: Sadat Noori,Ahmad Monabati,Abbasali Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Estradiol,stereology,Sperm,Testosterone,Matricaria chamomilla
- چکیده:
- چکیده انگلیسی: Background: Matricaria chamomilla (chamomile) is a herb used to treat various human illnesses. The present study was conducted to investigate the effects of chamomile extract on spermatozoon quality, serum levels of estradiol and testosterone, and sperm tail length in male adult rat. Methods: Male Sprague-Dawley rats received extract of chamomile (400 mg/kg once daily, orally) during an 8-week period, while the control animals received water. After this period, the animals were sacrificed and the blood samples were obtained. The serum levels of testosterone, estradiol, and the number, motility, and morphology of spermatozoon were assessed. The spermatozoon tail length was assessed by a rapid stereological method. Results: The body weight, and weight, and volume of the testis in the control and experimental rats did not change significantly. Serum testosterone level was decreased (~76%, P<0.005) and the serum estradiol level was increased (~16%, P<0.04) in the experimental animals. The spermatozoon count and motility were decreased in the experimental group but spermatozoon morphology did not show significant changes. The mid-piece and total tail length were reduced in the experimental group (~22%, P<0.001). Conclusion: Matricaria chamomilla extract can decrease spermatozoa count and motility, spermatozoon tail length, serum testosterone level and increase serum estradiol level in male adult rat.
- انتشار مقاله: 16-12-1393
- نویسندگان: Saied Karbalay-Doust,Ali Noorafshan,Farzaneh Dehghani,Mohamad Reza Panjehshahin,Ahmad Monabati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Echinococcus granulosus is considered the major cause of human hydatid cysts. Usually the duration of cyst formation is 10-20 years. This period shortens significantly upon rupture of a primary cyst. The literature describes low incidence of primary involvement of ovary as a site of hydatid cyst formation. Our case is the first report on ovarian hydatid cyst in Iran. A 60-year-old woman was presented with abdominal pain in the left lower quadrant area. Paraclinical data were suggestive of neoplasia and preoperative diagnosis was ovarian tumor. During laparotomy, multiple cysts resembling hydatid cysts were observed in the left ovary. Pathological examination confirmed the diagnosis of hydatid cyst. Although there is a small possibility of secondary ovarian echinococcal disease, it is more probable for this case to be primary infection, as the patient had developed ovarian hydatid cysts 15 years after hepatic involvement and recurrence after 30 months is very uncommon.
- انتشار مقاله: 22-02-1394
- نویسندگان: Mohsen Khosravi Maharlooei,Armin Attar,Amita Goran,Seddigheh Amuee,Arman Dehghan,Ahmad Monabati
- مشاهده