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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Frequency,Iran,HLA-G*0105N antigen,RFLPs
- چکیده:
- چکیده انگلیسی: Background: HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to in-hibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace their roles. Stud-ies on the null allele of HLA-G gene could be useful in understanding the genetic vari-ants of HLA-G alleles in ethnic groups.
Objective: The goal of this research was to de-termine the frequency of HLA-G*0105N null allele in Iranian healthy subjects. Meth-ods: The frequency of HLA-G*0105N null allele was evaluated in Iranian healthy sub-jects by PCR-RFLP method. Genomic DNA was isolated from the whole blood of 100 randomly selected, healthy, unrelated Iranian individuals using salting-out technique followed by PCR amplification of the exon 3 of HLA-G gene. PCR products were di-gested with PpUM-1 and the resulted fragments were analyzed using gel electrophore-sis.
Results: In this study the restriction enzyme digestion confirmed homozygous HLA-G*0105N null allele for 9 % of the population. Furthermore obtained results indi-cated that the total frequency of HLA-G*0105N null allele was 20 % in the studied population of Iran.
Conclusion: The final data analysis showed that the total frequency of this allele in Iranian people was higher than other ethnic groups that have been stud-ied so far.- انتشار مقاله: 17-05-1395
- نویسندگان: Roghayeh Rahimi,Ahmad Zavaran Hosseini,Fatemeh Yari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Toxoplasma gondii,Immune responses,Mice Peritoneal Exudates,Excreted/secreted Antigens
- چکیده:
- چکیده انگلیسی: Background: Toxoplasma gondii is an obligate intracellular parasite that infects all mammalian cells. Several antigens such as excreted/secreted antigens have been identified as a potential vaccine candidate.
Objective: To determine how excreted/secreted antigens from peritoneal exudates of infected mice (mESA) stimulate cell-mediated immune responses and induce protective immunity against toxoplasmosis in the murine model.
Methods: The supernatants produced from the peritoneal fluids, were fractionated by precipitation in ammonium sulphate solution (30-80% saturated). For induction of cell-mediated immune responses, delayed type hypersensitivity was measured, in injected footpad. Response to purified antigen was measured by lymphocyte proliferation assay. Nitric oxide was measured by Griess method. For immunization, Balb/c mice were immunized 2 times with mESA, mESA-40% and Toxoplasma Lysate Antigen (TLA). The virulent RH strain of Toxoplasma gondii was used for challenging.
Results: The pattern of lymphocyte responsiveness was dependent on the antigen employed. In sensitized mice, those received mESA-40% displayed higher lymphocyte response than mice stimulated by mESA (p<0.05). The highest amounts of nitric oxide were observed in macrophages, which received mESA-40% and mESA (p<0.05). Mice immunized with mESA-40% survived longer than those immunized with mESA and other antigens (p<0.05).
Conclusion: As fraction 40% (mESA-40%) showed a good result in induction of cellmediated responses in the murine model, the purification and isolation of the mESA 40% is highly recommended for future study.- انتشار مقاله: 16-05-1395
- نویسندگان: Ahmad Daryani,Ahmad Zavaran Hosseini,Mehdi Sharif,Abdolhoseein Dalimi,Mohammad Hossein Dehghan,Hajar Ziaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Lymphoma,phage display,Drug delivery system,Nanobody,CD19
- چکیده:
- چکیده انگلیسی: Objective(s): CD19 is a transmembrane glycoprotein of immunoglobulin superfamily. In order to treat lymphoma, monoclonal antibodies (mAb) can target different antigens, including CD19, CD20 and CD22 on the surface of B-cells. Along with biotechnology progress, a new generation of antibodies is introduced, with the purpose of eliminating the defects of the previous generation. Among the most developed one are nanobodies (Nb). Nbs are a unique kind of camelid single domain antibody fragments with a broad range of medical applications. Unique physicochemical properties of Nbs have made them ideal candidates for therapeutic and diagnostic applications.
Materials and Methods: An immune gene library was created, and several CD19 specific Nbs were selected through antigen panning process, and their molecular properties as well as specificity, sensitivity, affinity and immunoreactivity against CD19 positive and negative cells were evaluated.
Results: The Nb library was prepared with 7.2 x107 members. We managed to isolate a panel of CD19-specific Nbs after the last round of selection with the affinity of isolated Nbs being estimated at the standard range of 15-35 nM. Sequence analysis of positive clones was indicative of the fact that 12 variable sequences were confirmed. Of all these 12 clones, 2 clones with the greatest level signal in ELISA underwent subsequent analysis. Our sequencing results indicated high sequence homology (approximately 90%) between the Nb and Homa variable immunoglobulin domains.
Conclusion: Specific Nbs possess the potential to be used as novel therapeutic approaches in order to treat autoimmune diseases and B-cell lymphoma.- انتشار مقاله: 17-07-1396
- نویسندگان: Seyed Reza Banihahsemi,Ahmad Zavaran Hosseini,Fatemeh Rahbarizadeh,Davoud Ahmadvand
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Insulin resistance,diabetes,Metabolic Syndrome,Adiponectin,Free fatty acids
- چکیده:
- چکیده انگلیسی: Objective(s): This study was aimed to investigate the effect of Berberis integerrima (B. integerrima) extract on insulin sensitivity in high-fructose-fed insulin-resistant rats.
Materials and Methods: Experimental rats were randomly divided into two groups: the control group was fed a regular chow diet while other group fed with a high-fructose diet for 8 weeks. After the first six weeks, the animals were treated with B. integerrima extract or pioglitazone for two weeks. Insulin and adiponectin levels were measured by ELISA. Additionally, Insulin resistance was calculated using a Homeostasis Model Assessment of Insulin resistance (HOMA-IR). The plasma free fatty acid (FFA) profile was obtained by gas chromatography. PPARγ and GLUT4 gene expression were assessed by real-time polymerase chain reaction (PCR) and western-blotting.
Results: Comparing the B. integerrima treated group with the control group, weight gain (P=0.009) and levels of insulin (P=0.001), blood glucose (P<0.0001), and HOMA-IR (P<0.0001) were significantly reduced. Additionally, the adiponectin concentration was significantly increased (P<0.0001). Among the FFA fractions, the mean concentration of palmitoleic acid and stearic acid in the B. integerrima group were significantly higher than the control group (P<0.0001 and P=0.005, respectively). However, there was no significant difference at the mRNA and protein level of GLUT4 and PPAR-γ between B. integerrima treated group and control group.
Conclusion: The study findings revealed that B. integerrima might be a protective candidate against Type 2 diabetes/insulin resistance through direct insulin-like effect and an increase in adiponectin levels. However, the mechanism of B. integerrima was independent of GLUT4 and PPARγ.- انتشار مقاله: 03-07-1396
- نویسندگان: Hossein Fallah,Hamed Akbari,Moslem Abolhassani,Abbas Mohammadi,Ahmad Gholamhosseinian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Vagus nerve,Melatonin Ischemia/reperfusion
- چکیده:
- چکیده انگلیسی: Objectives:Vagal pathways in gastrointestinal tract are the most important pathways that regulate ischemia/reperfusion (I/R). Gastrointestinal tract is one of the important sources of melatonin production. The aim of this study was to investigate probable protective effect of the interaction between vagus nerve and melatonin after I/R.
Materials and methods:This study was performed in male rats that were divided into six groups. Cervical vagus nerve was cut bilaterally after induction of I/R and the right one was stimulated by stimulator. Melatonin or vehicle was injected intraperitoneally. The stomach was removed for histopathological and biochemical investigations.
Results: A significant decrease in infiltration of gastric neutrophils and malondialdehyde (MDA) level after I/R was induced by melatonin and was disappeared after vagotomy. The stimulation of vagus nerve significantly enhanced these effects of melatonin. However, a stimulation of vagus nerve alone increased neutrophils infiltration and MDA level. Melatonin significantly increased the activities of catalase, glutathione peroxidase (GPx), superoxide dismutases (SOD). Unlike stimulation of vagus nerve, vagotomy decreased these effects of melatonin.
Conclusion:According to these results, it is probable that protective effects of melatonin after I/R may be mediated by vagus nerve. Therefore, there is an interaction between melatonin and vagus nerve in their protective effects.- انتشار مقاله: 10-11-1394
- نویسندگان: Nader Shahrokhi,Mohammad Khaksari,Shahla Nourizad,Nava Shahrokhi Shahrokhi,Zahra Soltani,Ahmad Gholamhosseinian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Nitric oxide,Iron,Lipopolysaccharide,Macrophage,Interferon gamma
- چکیده:
- چکیده انگلیسی: Objective(s) Previous studies have demonstrated that the nitric oxide (NO) dependent death of murine peritoneal macrophages activated in vitro with IFN-g and LPS is mediated through apoptosis. In the present study, we investigated the synergistic effect of LPS, IFN-g and iron on NO production and apoptosis. Materials and Methods After determination of iron cytotoxicity, the peritoneal macrophages of Balb/c mice were cultured with iron, LPS, and IFN-g separately, or a mixture of these for 18 hr at 37 ◦C. Then after 18 hr incubation, the level of NO in supernatant was measured by the Griess method. At the same time, after incubation with ethidium bromide and acridine orange dye, the apoptotic macrophages were detected by fluorescence microscopy. Results NO production was significantly greater than the control group in macrophages exposed to iron,LPS, or IFN-g alone (P=0.02), while no significant difference was detected in apoptosis rate in the presence of LPS (P=0.08). However, the differences were remarkable between NO production and apoptosis rate in the presence of iron, LPS and IFN-g (P≤0.05). Conclusion These findings indicate the immunostimulatory effect of iron on NO production by IFN-g and LPS.
- انتشار مقاله: 14-07-1394
- نویسندگان: Leila Pirdel,Ahmad Zavaran Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: children,aggression,adolescents,life satisfaction,self-rated health
- چکیده:
- چکیده انگلیسی: Background: Weight loss and decreasing the Body fat percentage (BF%) is motivated to optimize performance. In order to achieve these, many supplements are used by athletes, however the possible negative or synergic effects have not been fully described in the literature, specifically in humans. The present study was conducted to investigate the co-administration effects of two common used supplements in body weight and BF% management to recommend athletes for safe weight and BF% reduction.
Materials and Methods: In the present double-blind, randomized, parallel, placebo-controlled study, the effect of six-week co-administration of caffeine and carnitine was determined on changes in body weight (BW), BF%, serum leptin concentration and lipid profile (triglyceride, HDL Cholesterol, LDL Cholesterol and Total Cholesterol), fasting blood glucose (FBG), and free fatty acid (FFA) changes. Twenty eight male teen soccer players from Ahvaz-Iran, were divided in three groups (group CafPlc, caffeine (6 mg/kg/day) + dextrose; group CafCar, caffeine (6 mg/kg/day) + carnitine (2g); and group Plc, dextrose).
Results: Caffeine-carnitine had a lowering effect on BW (P=0.02) and BF% (P=0.03), compared to caffeine alone and placebo in male teen soccer players (mean age of 16.92 ± 0.76 years). TG was significantly decreased in CafCar (P=0.04). FFA levels were increased in CafCar (P=0.04) and there was significant differences between CafCar and Plc groups (P=0.01). FBG was increased in both CafPlc and CafCar (P=0.01 and P=0.02, respectively), with no significant differences between groups.
Conclusion: The synergistic effect of caffeine-carnitine might be suggested to decrease the BF% and BW, besides it may prevent the increment of FFA levels; however it should be prescribed cautiously since it increased FBG levels.- انتشار مقاله: 14-06-1395
- نویسندگان: Saeed Shirali,Sevana Daneghian,Seyed Ahmad Hosseini,Damoon Ashtary-Larky,Marina Daneghian,Maryam-Sadat Mirlohi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Agricultural Science Research& Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Erosion hazard index,Quantitative assessment,Stream condition,River indicator
- چکیده:
- چکیده انگلیسی: An erosion hazard index methodology (EHIM) was developed for assessing stream erosion. The index of stream erosion is designed as a management tool. Assessing stream erosion involves consideration of a range of aspects of streams and a value judgment about a desirable state. The assessment of the erosion indicators of streams was based on a state-wide assessment of physical stream condition. A scale from 0 to 100 was chosen as a basis for ranking where an erosion hazard index (EHI) of 100 indicates the very extensive possible erosion state and one of zero the stable or no erosion possible erosion state. In the EHIM five steps are necessary for calculation: to measure and select of basic and additional indicators, to calculate sub-EHIi for all selected indicators, to determine weighting factors for all selected indicators, to calculate a synthetic EHI using the sub-EHIi and weighting factors for all selected indicators and final step: to assess stream erosion based on synthetic EHI values. The EHIM was applied to a 378 number of Ardabil Province (NW of IRAN) for assessment and comparison purposes. Length of stream erosion (LE) was selected to serve as a basic indicator, while erosion susceptibility of lithology (LESi), length of streams with lateral erosion (LLAE), length of streams with bed erosion (LB), the plant cover and human impacts (LAHE), pasture lands (LPE) and their relationships and discharge of floods (Q2.33) were used as additional indicators. The results suggest that the EHIM is a valuable relatively uncomplicated methodology with simple principles, ease of calculation, reliable and intuitive results. As a practical planning tool, it can be widely used for the quantitative assessment and comparison of stream erosion states for a series of different streams or more complicated stream systems .However, planning for river management systems is complicated by a variety of uncertainties but this paper presents the development of a simple assessment model for river management under uncertainty. [Talaei et al. Erosion Hazard Index Methodology (EHIM) for Streams Erodibility Assessment (Ardabil-Province). International Journal of Agricultural Science, Research and Technology, 2012; 2(2):89-97].
- انتشار مقاله: 04-10-1394
- نویسندگان: Reza Talaei,Farzaneh Azimi,Seyed Ahmad Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Cell line establishment,Triple-negative
- چکیده:
- چکیده انگلیسی: Breast cancer is the most common malignancy and the leading cause of cancer-related death among women
worldwide. The underlying mechanisms for breast cancer development, especially in young women, are not completely
understood. Although there are several experimental models to understand the biology of breast cancer such as
immortalized cell lines, many of these cell lines have been in culture for decades and most of them have been derived
from Caucasians or African-Americans. So, it is required to establish a new cell line derived from primary tumors and
Asian women. In this study Pari-Institute for Cancer Research (Pari-ICR) was derived from the primary breast tumor
of a 36-years old patient with invasive ductal carcinoma. We characterized the cell line by examining morphology,
expression of different markers, and functional profile. Immunocytochemistry showed that this cell line does not express
estrogen and progesterone receptors as well as human epidermal growth factor receptor 2 (HER2). Pari-ICR cell line
expresses high levels of Vimentin, Ezrin, and S100 but does not express EpCAM, Cytokeratin19, Pan-cytokeratin,
Nestin, and Desmin. Its doubling time of Pari-ICR was about 22h and was able to grow as colonies in soft agar. It
displayed a higher ability of migration and invasion in comparison with MCF-7 cell line. This breast cancer cell line
can serve as a model for understanding the molecular mechanisms of breast carcinogenesis. Moreover, it can be used
as an appropriate resource to find novel biomarkers or assess new drugs.- انتشار مقاله: 10-05-1397
- نویسندگان: Farzaneh Ghaderi,Fereshteh Mehdipour,Ahmad Hosseini,Abdolrasoul Talei,Abbas Ghaderi
- مشاهده