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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Superoxide Dismutase,Catalase,Malondialdehyde,Ferula gummosa,root
- چکیده:
- چکیده انگلیسی: Ferula gummosa Boiss is a good source of biologically active compounds such as monoterpene and sesquiterpene derivatives. There are also several reports on antioxidant effects of these compounds. The aim of this study was to investigate the effect of daily administration of F. gummosa root hydro-alcoholic extract on serum oxidant-antioxidant status. Twenty four Wistar rats were randomly divided into three groups: (1) control, (2) F. gummosa extract 100 mg/kg, and (3) F. gummosa extract 600 mg/kg. The extract was administered by orogastric gavage once daily for 28 consecutive days. The activity of catalase and superoxide dismutase (SOD) enzymes, and the level of malondialdehyde (MDA, as a marker of lipid peroxidation) and total thiol groups were evaluated in blood samples of fasting animals on day 0 and day 28. F. gummosa extract at both doses significantly increased the activity of catalase (p<0.01). The extract at dose of 600 mg/kg significantly increased the activity of SOD (p<0.05), and reduced the level of MDA. F. gummosa had no effect on content of total thiol groups. In conclusion, long-term consumption of hydro-alcoholic extract of F. gummosa root increases the defense of the body against oxidative stress by increasing the activity of catalase and SOD, and by reducing lipid peroxidation.
- انتشار مقاله: 22-09-1394
- نویسندگان: Ahmad Ghorbani,Marzieh Mogharrabi,Reza Mohebbati,Seyed Mojtaba Mousavi,Shima Hasan Zadeh,Marzieh Emamian,Atieh Baharloo,Mohammad Soukhtanloo
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Apoptosis,DNA,PC12,Rutin
- چکیده:
- چکیده انگلیسی: Objective(s): Rutin is a flavonoid with potent antioxidant property, which exhibited cytoprotective effects in several models of neuronal injury. This work aimed to examine whether rutin can protect neurons against oxidative DNA damage caused by serum/glucose deprivation (SGD) as an in vitro model of neurodegeneration and ischemia.
Materials and Methods: The PC12 cells were cultured for 2 hr in normal culture medium containing different concentrations of rutin or α-tocopherol (positive control) and then further incubated for 12 hr in SGD condition. Then, cell viability, DNA fragmentation, lipid peroxidation, generation of reactive oxygen species (ROS), and the expression of proteins involved in apoptosis were determined.
Results: The SGD condition significantly decreased viability of the cells, which was accompanied by a significant rise in the generation of ROS and lipid peroxidation. Rutin enhanced the viability of PC12 cells in SGD condition and reduced the production of ROS and lipid peroxidation. In addition, rutin decreased DNA damage and inhibited apoptotic cell death by decreasing the levels of proapoptotic proteins (Bax, caspase-3, caspase-9) and increasing the level of anti-apoptotic protein Bcl-2.
Conclusion: This study demonstrated that rutin inhibits oxidative DNA damage and neuronal death induced by nutrients deprivation condition. Further studies may warrant the use of rutin as an appropriate neuroprotective agent for ischemic attacks and other neurodegenerative disorders.- انتشار مقاله: 23-02-1397
- نویسندگان: Marjan Nassiri-Asl,Ahmad Ghorbani,Sahar Salehisar,Elham Asadpour,Hamid Reza Sadeghnia
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Apoptosis,DNA,PC12,Rutin
- چکیده:
- چکیده انگلیسی: Objective(s): Rutin is a flavonoid with potent antioxidant property, which exhibited cytoprotective effects in several models of neuronal injury. This work aimed to examine whether rutin can protect neurons against oxidative DNA damage caused by serum/glucose deprivation (SGD) as an in vitro model of neurodegeneration and ischemia.
Materials and Methods: The PC12 cells were cultured for 2 hr in normal culture medium containing different concentrations of rutin or α-tocopherol (positive control) and then further incubated for 12 hr in SGD condition. Then, cell viability, DNA fragmentation, lipid peroxidation, generation of reactive oxygen species (ROS), and the expression of proteins involved in apoptosis were determined.
Results: The SGD condition significantly decreased viability of the cells, which was accompanied by a significant rise in the generation of ROS and lipid peroxidation. Rutin enhanced the viability of PC12 cells in SGD condition and reduced the production of ROS and lipid peroxidation. In addition, rutin decreased DNA damage and inhibited apoptotic cell death by decreasing the levels of proapoptotic proteins (Bax, caspase-3, caspase-9) and increasing the level of anti-apoptotic protein Bcl-2.
Conclusion: This study demonstrated that rutin inhibits oxidative DNA damage and neuronal death induced by nutrients deprivation condition. Further studies may warrant the use of rutin as an appropriate neuroprotective agent for ischemic attacks and other neurodegenerative disorders.- انتشار مقاله: 23-02-1397
- نویسندگان: Marjan Nassiri-Asl,Ahmad Ghorbani,Sahar Salehisar,Elham Asadpour,Hamid Reza Sadeghnia
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Alendronate,Bisphosphonates,Human dental pulp stem cells
- چکیده:
- چکیده انگلیسی: Objective(s): Osteonecrosis of the jaw, as an exposed necrotic bone in the oral cavity, is one of the adverse effects of bisphosphonates, which have an affinity for bone minerals. This study investigates the cytotoxic effects of alendronate (ALN) as a nitrogen-containing bisphosphonate, on human dental pulp stem cells (hDPSCs).
Materials and Methods: The mesenchymal stem cells (MSCs), obtained from third molar tooth pulps were characterized by immunophenotyping assay in order to identify surface markers to evaluate their expression. To detect multipotency hDPSCs, they were differentiate into osteocytes and adipocytes. Cell proliferation was measured by MTT assay. PI staining of DNA fragmentation by flowcytometry (sub-G1 peak) was performed for determination of apoptotic cells and Bax, Bcl-2, and cleaved caspase 3 expressions. Protein expression was detected by Western blotting.
Results: As the results revealed, ALN decreased viable cells (in 0.8–100 µM) after 72 hr and 168 hr (P<0.001), significantly. ALN could lower cell proliferation in hDPSCs in a concentration and time-dependent manner. Sub-G1 peak as an indicator of flowcytometry histogram of treated cells by ALN, showed apoptosis was involved in ALN-induced cytotoxicity. Expressions of cleaved caspase 3 and Bax protein, as pro-apoptotic proteins, were increased and Bcl-2 protein as anti-apoptotic protein was decreased in response to increases in the concentration of ALN (0.8–25 µM).
Conclusion: Long-term effects of ALN on cell proliferation and apoptosis in hDPSCs can result in either initiation or potentiation of ALN-induced osteonecrosis.- انتشار مقاله: 19-01-1396
- نویسندگان: Solmaz Pourgonabadi,Ahmad Ghorbani,Zahra Tayarani Najaran,Seyed Hadi Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Crocus sativus,Safranal,Hippocampus,Neurodegenerative disorders,Quinolinic acid
- چکیده:
- چکیده انگلیسی: Objective(s): Quinolinic acid (QA)-mediated excitotoxicity has been widely used as a model for studying neurodegenerative disorders. Recent studies suggested that saffron (Crocus sativus) or its active metabolite, i.e. safranal, exerts pharmacological actions on central nervous system including anxiolytic, anticonvulsant, and neuroprotective properties. The present study aimed to investigate the effect safranal pretreatment on QA-induced oxidative damage in rat hippocampus.
Materials and Methods: Under anesthesia, a guide cannula was stereotaxically inserted into left ventral hippocampus of rats. The rats were then given either saline or safranal (72.75, 145.5, and 291 mg/kg, IP) 30 min before administration of QA (300 nmol, intrahippocampal injection). The markers of oxidative stress including thiobarbituric acid reactive substances (TBARS, as an index of lipid preoxidation), total sulfhydryl groups, antioxidant capacity of hippocampus (using FRAP assay), and oxidative DNA damage (%tail DNA, using comet assay) were measured in hippocampus.
Results: The QA induced a significant increase in TBARS levels and %tail DNA and remarkable decrease in antioxidant power (FRAP value) and total sulfhydryl content of hippocampus, in comparison with control animals. Systemic administration of safranal (291 mg/kg, IP), effectively and dose-dependently decreased the QA-induced lipid peroxidation (P<0.001) and oxidative DNA damage (P<0.001). Safranal also prevented the decrease of hippocampal thiol redox and antioxidant status (P<0.001) produced by QA.
Conclusion: Safranal have protective effects on different markers of oxidative damage in hippocampal tissue following QA administration. Our findings might raise a possibility of potential therapeutic application of safranal for preventing and treating neurodegenerative disorders such as Alzheimer’s disease.- انتشار مقاله: 25-11-1391
- نویسندگان: Hamid Reza Sadeghnia,Mina Kamkar,Elham Assadpour,Mohammad Taher Boroushaki,Ahmad Ghorbani
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Mining and Geo-Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Lattice,Numerical Simulations,Hydraulic fracture,Orthogonal Fractures,Non- Orthogonal Interfaces
- چکیده:
- چکیده انگلیسی: The hydraulic fracturing propagation is strongly influenced by the existence of natural fractures. This is a very important factor in hydraulic fracturing operations in unconventional reservoirs. Various studies have been done to consider the effect of different parameters such as stress anisotropy, toughness, angle of approach and fluid properties on interaction mechanisms including crossing, arresting and opening. Analytical solutions can only be used for simple fracture geometries and are not usually able to provide good predictions due to many simplified assumptions. Laboratory tests are also conducted under certain constraints like sample size and conditions that are different from the real field conditions. Numerical simulations, including continuum and dis-continuum based models have been used extensively to simulate hydraulic fracture propagation and its interaction with natural interfaces. However, calibration of simulated models with real field data is necessary to ensure the accuracy of the results. A calibrated numerical simulation can be used to model complex geometries. In this study, a Lattice numerical simulator, which is the advanced version of Particle flow Code (PFC) based on the granular particle physics, was used for numerical simulation of lab scale hydraulic fracturing. The scaling laws were also used to increase the dimensions of the simulated samples to allow increasing the rate of fluid injection and reducing its viscosity, hence reduce the simulation time. The interaction of hydraulic fractures and orthogonal fractures with angles of approach of 90°, 60° as well as non-orthogonal fracture planes with different filling materials ranging from strong to very weak were studied. The results showed good agreement with lab observations. In general the larger the angle of approach and stronger the filling material, the higher the likelihood of the crossing mode. Also, networks of regular natural fractures with two fracture sets were simulated. The results showed that the combination of different parameters define the preferred fracture propagation (PFP) which is not easy to predict using analytical solutions. In this situation and more complex real field cases, the use of numerical simulations are necessary to predict the propagation of hydraulic fracture and interaction modes.
- انتشار مقاله: 17-09-1397
- نویسندگان: Elham Bakhshi,Vamegh Rasouli,Ahmad Ghorbani,Mohammad Fatehi Marji
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Mining and Geo-Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: finite element method,Numerical modelling,Sechahoon Iron Mine,Fractured Formation,Feflow
- چکیده:
- چکیده انگلیسی: Considering the fact that a large volume of iron reserve in the Sechahoon Iron Mine in Yazd Province has located under the water table, it is necessary to conduct a comprehensive study on water flow within the pit and its surroundings. The conceptual model of the aquifer was created using surface and underground geological information compared with water table data of the area of interest. In the data preparation stages, in order to create the numerical model, Logan and Lufran tests were studied to determine the hydrodynamic coefficients of the layers, precipitation and evaporation were investigated, and fractures and faults of the region, as a medium for flow channels in the hard formation, were also studied. The model was created in a transient state between 2000 and 2014. To validate its results, the water table was measured 4 times in the last 4 months of 2014. Considering the complexities in the heterogeneous fractured aquifer of the study area, numerical modeling results for the basin in a transient state present 90 percent correlation with field studies. Having investigated the water balance in the region, the boundary condition of the model was determined as the input water from the eastern south and the runoff water in the western north of the region. Since the general trend of faults in the area is north-south, variation in the water table is slight on north-south and intense on the east-west direction. On the other hand, due to the fact that the maximum flow is along the faults and fractures, the water table contour lines in different locations over the region are closed.
- انتشار مقاله: 05-11-1394
- نویسندگان: Mojtaba Darabi,Abdolhamid Ansari,Nader Fathianpour,Ahmad Ghorbani
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Cytotoxicity,Glioblastoma Multiforme,Brain tumors,Auraptene
- چکیده:
- چکیده انگلیسی: Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line.
Materials and Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κB, IL-1β, and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR).
Results: The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC50 values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p real-time RT-PCR showed an up-regulation in Bax, NF-κB, IL-1β, and p53 but a down-regulation in MCP-1 and Bcl-2 genes expression.
Conclusion: This study showed that auraptene triggered apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, auraptene can be utilized as an effective natural medicine against GBM, after complementary studies.- انتشار مقاله: 01-05-1397
- نویسندگان: Amir R. Afshari,Mostafa Karimi Roshan,Mohammad Soukhtanloo,Ahmad Ghorbani,Farzad Rahmani,Mohammad Jalili-nik,Mohammad Mahdi Vahedi,Azar Hoseini,Hamid R. Sadeghnia,Hamid Mollazadeh,Seyed Hadi Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,ROS,H9C2 cells,Pomegranate seed oil,SOD
- چکیده:
- چکیده انگلیسی:
Objective: It has been well documented that oxidative stress is involved in the pathogenesis of cardiac diseases. Previous studies have shown that pomegranate seed oil (PSO) has antioxidant properties. This study was designed to investigate probable protective effects of PSO against hydrogen peroxide (H2O2)-induced damage in H9c2 cardiomyocytes.
Materials and Methods: The cells were pretreated 24 hr with PSO 1 hr before exposure to 200 µM H2O2. Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. The level of reactive oxygen species (ROS) and lipid peroxidation were measured by fluorimetric methods.
Results: H2O2 significantly decreased cell viability which was accompanied by an increase in ROS production and lipid peroxidation and a decline in superoxide dismutase activity. Pretreatment with PSO increased viability of cardiomyocytes and decrease the elevated ROS production and lipid peroxidation. Also, PSO was able to restore superoxide dismutase activity.
Conclusion: PSO has protective effect against oxidative stress-induced damage in cardiomyocytes and can be considered as a natural cardioprotective agent to prevent cardiovascular diseases.
- انتشار مقاله: 11-07-1394
- نویسندگان: Mehdi Bihamta,Azar Hosseini,Ahmad Ghorbani,Mohammad Taher Boroushaki
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Seizures,Coriandrum sativum,Pentylenetetrazole,Frakshens
- چکیده:
- چکیده انگلیسی: Objective: In the present work, the effects of different fractions of Coriandrum sativum (C. sativum), on pentylenetetrazole (PTZ)-induced seizures and brain tissues oxidative damage were investigated in rats.
Materials and Methods: The rats were divided into the following groups: (1) vehicle, (2) PTZ (90 mg/kg), (3) water fraction (WF) of C. sativum (25 and 100 mg/kg), (4) n-butanol fraction (NBF) of C. sativum (25 and 100 mg/kg), and (5) ethyl acetate fraction (EAF) of C. sativum (25 and 100 mg/kg).
Results: The first generalized tonic-clonic seizures (GTCS) latency in groups treated with 100 mg /kg of WF or EAF was significantly higher than that of PTZ group (p< 0.01). In contrast to WF, the EAF and NBF were not effective in increasing the first minimal clonic seizure (MCS) latency. Malondialdehyde (MDA) levels in both cortical and hippocampal tissues of PTZ group were significantly higher than those of control animals (p< 0.001). Pretreatment with WF, NBF, or EAF resulted in a significant reduction in the MDA levels of hippocampi (pConclusion: The present study showed that different fractions of C. sativum possess antioxidant activity in the brain and WF and EAF of this plant have anticonvulsant effects.- انتشار مقاله: 17-01-1394
- نویسندگان: Akbar Anaeigoudari,Mahmoud Hosseini,Reza Karami,Farzaneh Vafaee,Toktam Mohammadpour,Ahmad Ghorbani,Hamid Reza Sadeghnia
- مشاهده