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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Matrix assisted laser desorption ionization/ time of flight mass spectrometry,magnetic beads,ClinProTools
- چکیده:
- چکیده انگلیسی: Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed
the plasma proteomic profiling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography
magnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight mass
spectrometry (MALDI-TOF MS). Results: ClinProTools software identified 92 peaks that differed among the analyzed
groups, 33 peaks were significantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks were
down-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4
and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjects
with 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of
100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishing
BC patients from healthy controls.- انتشار مقاله: 24-04-1397
- نویسندگان: Ahmad Zaki,Ragaa Abdelkader Ramadan,Pacint Moez,Hala Ghareeb,Ahmad Elkarmouty
- مشاهده