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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Tissues and Materials
- نوع مقاله: Journal Article
- کلمات کلیدی: Endometriosis,Iranian women,gene polymorphism,ARMS-PCR
- چکیده:
- چکیده انگلیسی: Endometriosis is a benign gynecologic disorder that has malignant-like characteristics such as invasion, and it can cause malignancy and cancer in advanced stages. Genetic, epigenetic and environmental factors play significant roles in the establishment and maintenance of endometriosis. Studies have demonstrated that proto-oncogenes may be an important regulator of cell proliferation in endometriotic tissue. B-Raf is a proto-oncogene, which participates in the MAP kinase/ERK signaling pathway and plays an important role in different types of cancers and disorders. The aim of the present study was to evaluate the association of BRAF gene single nucleotide polymorphism (SNP), rs113488022, with the risk of endometriosis in Iranian women, in order to identify a potential biomarker for the early non-invasive detection of endometriosis and endometriosis-related cancers.
In a population based case-control study conducted in Tehran, in-person interviews were completed for 65 women aged 15– 49 years and an equal number of controls frequency-matched to cases by age. All cases were newly diagnosed with endometriosis and all controls were with no laparoscopic evidence of disease. The genomic DNA was extracted from peripheral blood leucocytes and subsequently the rs113488022 SNP of the BRAF gene was genotyped using amplification refractory mutation system- polymerase chain reaction (ARMS-PCR). Statistical analysis was performed using SPSS. In comparison of case and control groups, no significant differences were found in genotype and allele frequencies. Moreover, the results showed that there was no significant association between the presence of risk allele (A) and increased risk of endometriosis (Odds ratio=0.781, 95% CI: 0.480 -1.272, P=0.321).
In conclusion, there was no evidence of an association between the rs113488022 SNP of the BRAF gene, and overall risk of endometriosis in Iranian population. Larger studies with different ethnics are needed to validate our findings.- انتشار مقاله: 11-03-1397
- نویسندگان: Maryam Sadat Hoseini,Maryam Rashidnahal,Ahmad Ebrahimi,Gholamreza Javadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: treatment,Phenylalanine,Phenylketonurias
- چکیده:
- چکیده انگلیسی: Abstract Background: Molecular imprinting is a method for synthesizing polymers with structure-selective adsorption properties with applications such as, selectivity binding, drug delivery systems and anti-bodies. The present study aims at optimizing the preparation of molecularly imprinted polymer (MIP) against l-phenylalanine, in order to increase phenylalanine-binding in Enzymatic Intestinal Simulated Fluid (ESIF). Methods: The MIP for l-phenylalanine, as a water-soluble template, was successfully synthesized without derivatization. Synthesization was done by a UV polymerization method in which methacrylic acid (MAA), as a functional monomer, and ethylene glycol dimethacrylate (EGDMA), as a cross-linker, were used in the presence of five different porogenic solvents including; acetonitrile, tetrahydrofuran (THF), chloroform, toluene and dimethyl sulfoxide (DMSO). The selectivity of the MIP was examined using 19 different amino acids in human serum and was evaluated by HPLC. In addition, morphological studies were conducted using SEM. Results: The results showed that the obtained MIP with acetonitrile had the highest capacity and selectivity compared with other solvents. The data indicated that Phe-binding to MIP was significantly more than the former binding to NIP in EISF (P≤0.05). Moreover, in comparison with NIP and control group, MIP showed a better selectivity and binding for Phe. This could be used for the reduction of Phe in human serum samples of Phenylketonuria.Conclusion: Our findings suggest that the MIP against Phe prepared with acetonitrile, showed a good selectivity and binding, which caused a reduction of blood Phe concentration in enzymatic simulated intestinal fluid and human serum sample of Phenylketonuria.
- انتشار مقاله: 06-10-1392
- نویسندگان: Parvaneh Najafizadeh,Soltan Ahmad Ebrahimi,Mohammad Reza Panjehshahin,Seyed Mahdi Rezayat Sorkhabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mutation,Dystrophic epidermolysis - bullosa,Type VII collagen
- چکیده:
- چکیده انگلیسی: Objective(s): Epidermolysis bullosa is one of the most important series of mechano-bullous heritable skin disorders which is categorized into four major types according to the layer that bullae forms within basement membrane zone. In dystrophic form of the disease, blisters are made in the sublamina densa zone, at the level of type VII collagen protein which produce anchoring fibrils. Type VII collagen gene is the only responsible gene for this form. The aim of this study was to survey causative mutations of type VII collagen gene among Iranian patients with epidermolysis bullosa. Materials and Methods: For this purpose, exons 73-75 were investigated by polymerase chain reaction followed by direct sequencing. Results: In current study, we found three different point mutations in type VII collagen alleles in 7 out of 50 patients. Four patients were homozygous for a new deletion which resulted in frame shift (p.Pro2089fs). Two patients were homozygous for a recurrent glycine substitution (p.G2031S) and one patient was detected with an allele carrying a substitution (p.R2069C). Conclusion: The results emphasized heterogeneity in the type VII collagen gene and will provide a sign for early diagnosis and future study of the disease pathogenesis.
- انتشار مقاله: 10-06-1395
- نویسندگان: Armita Kakavand Hamidi,Mohammad Moghaddam,Nasim Hatamnejadian,Ahmad Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Liver,Kombucha,UDPGT,Mice,Iran nonpharmacologic nursing care
- چکیده:
- چکیده انگلیسی: Background and aims: Kombucha is an ancient food and healing source with Asian origin. Kombucha consists of a wide range of acids, including vitamin C, organic materials, enzymes, and B-group vitamins, which has provided it with immense value. This study was aimed to investigate the effects of Kombucha tea consumption on hepatic UDPGT enzyme in mice. Methods: In this experimental study, 21 small male albino mice and CD-1 genus were used. Albino mice species were purchased from serum Institute in Karaj Hisarak. Mice with a weight between 18 to 25 g were selected. Animals were kept in triplex group in polycarbonate cages. Animal rooms were equipped with air-conditioner. Ambient temperature was retained at about 22°C, and humidity 50%. A light cycle was set at 12 hours brightness and 12 hours darkness. The intensive diet was used that produced by animal Pars feeds for mice feeding. Finally, UDPGA enzyme was measured. Results: Consumption of kombucha tea for seven days of experiment caused a significant increase in enzyme activity in mice liver UDPGT compared to the negative control group, from the first day until the seventh day of experiment. Conclusion: Kombucha tea induced the UDPGT enzyme; thus, it accelerated the detoxification of the body and should be cautioned about simultaneous administration of this beverage with some drugs (such as steroids, acetaminophen, cardio– vascular medicine, etc.) that they metabolize through conjugation. Medical community in this case must be justified and their opinion must be considered when people are using this tea. Finally, kombucha tea consumption did not increase the liver weight. So, UDPGT induction was not the reason of liver weight increase.
- انتشار مقاله: 01-12-1393
- نویسندگان: Mohsen Asadbeygi,Masoud Yarahmadi,Ahmad Adineh,Mahmoud Bahmani,Fariborz Kayhanfar,Mohammad Hassan Pipelzadeh,Ahmad Ebrahimi,Zahra Mosavi
- مشاهده