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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Histopathology,Colorectal cancer,Dysplasia,adenomatous polyp,Interobserver agreement
- چکیده:
- چکیده انگلیسی: Background & Objective: Most colorectal cancers (CRCs) arise from adenomatous polyps, and clinical management of this type of polyp is highly dependent on the reliability and validity of the pathological diagnosis. The aim of this study was to examine the interobserver agreement of five pathologists in assessing dysplasia in adenomatous polyps.
Methods: In this study, a total of 146 adenomatous polyps of patients undergoing colonoscopy were selected from hospitals of Shahid Beheshti University of Medical Sciences, Tehran, Iran between 2017 and 2018. Five pathologists independently classified adenomatous polyps according to histologic type, nuclear pseudostratification, mitotic activity, nuclear polarity, nuclear pleomorphism, nuclear shape, nucleolus, chromatin pattern, cytology grade, architectural features, dysplasia, and final diagnosis. The overall kappa statistic (k) was used to assess agreement among pathologists.
Results: The mean age of the patients was 62.06 ± 13.06 (mean ± SD) with a male-to-female ratio of 2.2:1. The most common site of resection was the sigmoid colon (28.1%). The highest agreement was found for dysplasia grade (k=0.415) and histologic type (k=0.401), whereas the lowest agreement was found for mitotic activity (k=0.185), nuclear shape (k=0.187), and nucleolus (k=0.196).
Conclusion: Our findings indicate that agreement among pathologists in assessing dysplasia in adenomatous polyps is within fair to moderate levels of agreement. Therefore, there is a vital need to better clarify the current diagnostic criteria.- انتشار مقاله: 06-07-1398
- نویسندگان: Tahmineh Mollasharifi,Mahsa Ahadi,Elena Jamali,Afshin Moradi,Parisa Asghari,Saman Maroufizadeh,Behrang Kazeminezhad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Pathology,staging,Liver biopsy,Inter-observer,grading
- چکیده:
- چکیده انگلیسی: Background & Objective: Liver biopsy is the main method for grading and staging liver disorders, but the effects of clinical information and optimal biopsy specimen size on interpretation remain contentious. The aim of the study was to evaluate the impact of clinical information and quality of liver specimen on inter-observer agreement for liver disease.
Methods: A total of 289 consecutive biopsy specimens from 2010 to 2017 were re-evaluated by five pathologists using the modified Ishak and non-alcoholic fatty liver diseases (NAFLD) activity score (NAS) systems. Detailed clinical information was extracted from medical records of patients and the size of all liver biopsy samples was recorded.
Results: Full agreement between primary diagnosis and final diagnosis was obtained in 214 cases (74%). The remaining cases, namely 22 (7.6%) and 53 (18.3%) biopsies had minor and major diagnostic discrepancies, respectively. The results showed that the overall agreement was significantly higher in cases with complete clinical information than patients without any clinical information and even with partial clinical information (P<0.001). Interestingly, no significant difference in inter-observer agreement was achieved with a length over 20 mm (P=0.181). However, the inter-observer variation significantly decreased when the number of portal tract was more than 10 (P=0.001).
Conclusion: This study identified the impact of clinical information and the number of portal tracts as the key factors to diagnosis. Therefore, request forms for liver biopsies should always be accompanied with the clinical history. Moreover, adequacy of biopsy specimens is very useful for accurate evaluation of samples by pathologists.- انتشار مقاله: 27-09-1397
- نویسندگان: Zeinab Kishani Farahani,Mahsa Ahadi,Behrang Kazeminejad,Tahmineh Mollasharifi,Malihe Saber Afsharian,Amir Sadeghi,Farahnaz Bidari Zerehpoosh,Elena Jamali,Niki Hasanzadeh,Abolfazl Movafagh,Arash Dehghan,Arsham Moradi,Afshin Moradi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Quality Control,Sperm Count,Andrology,Semen analysis,Medical Laboratory
- چکیده:
- چکیده انگلیسی: Background and Objective: Infertility refers to the failure in achieving pregnancy of a couple after one year of regular sexual intercourse without using a protection method. The purpose of this research work was to evaluate the current status of the test and quality control performance in semen analysis in selected laboratories.
Material and Methods: The semen analysis was performed in the Laboratory of Andrology in terms of macroscopic examination which include volume, color, viscosity, pH and acidity, and in terms of microscopy: the rate of sperm movement, the exact number of sperms per ml of semen, the percentage of sperm viability and movement, the presence of germ cells and white blood cells. Several questions for each part of the test were selected and answered by the director of the laboratories or andrology section supervisor.
Results: There was a wide range in the performance of selected medical laboratories in Tehran regarding the standards of semen analysis according to the World Health Organization (WHO) Laboratory Manual for the examination and processing of human semen, fifth edition in 2010. They followed the instructions related to the sample collection in about 70% of the evaluated parameters, initial macroscopic examination in about 87% of the selected subjects, and the microscopic evaluation of sperm in about 65% of the test parameters.
Conclusion: some laboratories do not follow the instructions of the WHO in performing semen analysis, and most of them do not follow the suggested methods in all parts of the test.- انتشار مقاله: 18-07-1397
- نویسندگان: Mahsa Ahadi,Fereshte Aliakbari,Saeedeh Latifi,Seyed Jalil Hosseini,Atossa Gharib,Abolfazl Movafagh,Zahra Abdolalian,Arash Dehghan,Arsham Moradi,Behrang Kazeminejad,Azadeh Rakhshan,Elena Jamali,Farzad Allameh,Afshin Moradi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: age,Grade,Central nervous system,Location,Oligodendrogliomas
- چکیده:
- چکیده انگلیسی: Background and Objectives: Gliomas are the most prevalent subgroup of primary brain tumors with a relatively high mortality. However, oligodendrogliomas have a better prognosis compared to other subtypes due to their sensitivity to chemotherapy. Considering the low incidence and the resulting lack of information about oligodendrogliomas, particularly in Iran, this study aimed at assessing their basic characteristics.
Methods:In this descriptive retrospective study, patients with definite diagnosis of oligodendroglioma were identified by reviewing the archives of pathology reports at the department of pathology of Shohada-e Tajrish Hospital during years 2008 to 2014. Age, gender, location, and the grade of the tumor were extracted and entered to the SPSS statistical software for analysis.
Results: A total of 182 patients, including 115 males (63.2%) and 67 females (36.8%), were included with a mean age of 38.5±13.36 years. Frontal lobe was involved in 53 patients (29.1%), parietal lobe in 31 (17.0%), temporal lobe in 22 (12.1%), frontoparietal area in 15 (8.2%), parieto-occipital area in 11 (6.0%), temporoparietal and frontotemporal areas each in 9 subjects (4.9%), occipital lobe in 5 (2.7%), and the brainstem in 4 (2.2%). Furthermore, 108 cases (59.3%) had grade-2 and the remaining74 patients (40.7%) had grade-3 anaplastic oligodendrogliomas. The mean age of subjects with brainstem oligodendrogliomas was significantly lower than the other patients (p=0.025).
Conclusion: Oligodendrogliomas commonly effects the frontal lobe, followed by the parietal and temporal lobes. The mean age of subjects with brainstem lesions was significantly lower than other patients. Age, gender or location of the tumor did not independently predict a higher grade lesion.- انتشار مقاله: 06-06-1395
- نویسندگان: Mahsa Ahadi,Afshin Moradi,Azadeh Rakhshan,Alireza Arefian,Mitra Rafizadeh,Hanieh Zham
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Dermatology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hair loss,Alopecia areata,androgenetic alopecia
- چکیده:
- چکیده انگلیسی: Background: Androgenic hair loss (AGA) and alopecia areata (AA) are common conditions which sometimes are histologically difficult to differentiate. This study was conducted to detect differentiating features of these two disorders with IHC analysis of T-cell subsets in the inflammatory infiltrates of alopecia areata and androgenetic alopecia.
Methods: This cross-sectional study was conducted at Shohadae-Tajrish Hospital in 2018. Twenty-eight cases of alopecia areata and 32 androgenic hair loss cases were evaluated. H&E and IHC staining for CD3, CD4, and CD8+ T-lymphocytes were performed. Lymphocytic densities were graded in a quintet grading system. The inflammation density in various areas of skin tissue was evaluated. Statistical analysis was performed using SPSS version 21.
Results: Peribulbar lymphocytic infiltration was seen in 88.5% of AA patients and 12.5% of AGA patients (P = 0.000). Presence of melanin, pigmented casts, and lymphocytes in follicular stela
were in favor of AA diagnosis. CD3, CD4, and CD8+ T-cells in follicular stela, peri-bulbar, and subcutaneous regions were higher and denser in AA patients, while CD3 and CD4+ T-cells around
sebaceous ducts were in favor of AGA diagnosis.
Conclusion: Peribulbar lymphocytic infiltration is among the most important differentiating features of AA and AGA. Infiltration of T-cells in various regions of skin tissue has high diagnostic
value. Histologic and immunohistochemical evaluation of skin tissues according to these factors can differentiate these two entities with high precision.
- انتشار مقاله: 13-08-1398
- نویسندگان: Azadeh Rakhshan,Afshin Moradi,Hedieh Peiroolia
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal polyps,adenomatous polyps,Serrated polyps,non-neoplastic polyps
- چکیده:
- چکیده انگلیسی: Aim: This study was designed to report epidemiologic findings of polyps in Iranian patients, and predict histology of polyp regarding to demographic and colonoscopic findings. Background: Classification of colorectal polyps had been revised in the past two decades and there is a need for polyp categorization in the Iranian Health System. Patients and methods: In this retrospective study, the medical records of patients with colonoscopic diagnosis of polyp in pathology departments of SBMU affiliated teaching hospitals were reviewed. Patient’s slides evaluated and demographics findings were assessed. The anatomical location, macroscopic appearance including size and histological assessment of all polyps were recorded. Results: From total number of 1106 polyps (detected in 862 patients), adenomatous polyps (638 [57.7%]) were the most prevalent findings, followed by colon mucosal tag (184[16.6%]), hyperplastic and serrated polyps (122[11%]), inflammatory polyps (110[9.9%]), hamartomatous (21[1.9%]), and malignant lesions (13[1.2%]). Multivariate logistic regression showed age (each one year increasing age; odds ratio [OR] = 1.026, 95%confidence interval [CI] = 1.016–1.036, p < 0.0001), location of polyp (right colon; OR = 1.905, 95%CI = 1.366–2.656, p < 0.0001), and polyp size of 5-10 mm (OR = 1.662, 95%CI = 1.214–2.276, p = 0.002), and polyp size of >10 mm (OR = 2.778, 95%CI = 1.750–4.411, p< 0.0001) were independently associated with neoplastic polyps. Also, polyp size of >10 mm (OR= 2.613, 95%CI= 1.083-6.307, p=0.033), tubulovillous pattern of polyp (OR= 3.508, 95%CI= 1.666-7.387, p=0.001) and villous pattern of polyp (OR= 10.444, 95%CI= 4.211-25.905, p
- انتشار مقاله: 23-08-1397
- نویسندگان: Mahsa Ahadi,Behrang Kazemi Nejad,Zeinab Kishani Farahani,Tahmineh Mollasharifi,Elena Jamali,Hamid Mohaghegh Shalmani,Arash Dehgan,Maliheh Saberi Afsharian,Amir Sadeghi,Abolfazl Movafagh,Roxana Boran,Azadeh Rakhshan,Arsham Moradi,Mohammad Hassan Heidari,Afshin Moradi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Target Therapy,medulloblastoma,Computational Biology,Differ,KEGG pathways
- چکیده:
- چکیده انگلیسی: Introduction: One of the major challenges in cancer treatment is the lack of specific and accurate treatment in
cancer. Data analysis can help to understand the underlying molecular mechanism that leads to better treatment.
Increasing availability and reliability of DNA microarray data leads to increase the use of these data in a variety of
cancers. This study aimed at applying and evaluating microarray data analyzing, identification of important pathways
and gene network for medulloblastoma patients to improve treatment approaches especially target therapy. Methods:
In the current study, Microarray gene expression data (GSE50161) were extracted from Geo datasets and then analyzed
by the affylmGUI package to predict and investigate upregulated and downregulated genes in medulloblastoma. Then,
the important pathways were determined by using software and gene enrichment analyses. Pathways visualization
and network analyses were performed by Cytoscape. Results: A total number of 249 differentially expressed genes
(DEGs) were identified in medulloblastoma compared to normal samples. Cell cycle, p53, and FoxO signaling pathways
were indicated in medulloblastoma, and CDK1, CCNB1, CDK2, and WEE1 were identified as some of the important
genes in the medulloblastoma. Conclusion: Identification of critical and specific pathway in any disease, in our case
medulloblastoma, can lead us to better clinical management and accurate treatment and target therapy.- انتشار مقاله: 17-05-1397
- نویسندگان: Fateme Shaanbanpour Aghamaleki,Behrouz Mollashahi,Nika Aghamohammadi,Nematollah Rostami,Zeinab Mazloumi,Hamidreza Mirzaei,Afshin Moradi,Mojgan Sheikhpour,Abolfazl Movafagh
- مشاهده