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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,enterotoxin genes,pathogenicity,Multiplex-PCR
- چکیده:
- چکیده انگلیسی: Staphylococcus aureus (S. aureus) is a pathogen in community-acquired or hospital infections. Hence, the identification of this pathogen in clinical samples is a health concern and demands continued surveillance and close monitoring. In the current study, S. aureus strains were isolated from various clinical specimens in the Shariati Hospital, Tehran, Iran. Samples were studied to discover S. aureus enterotoxin-coding genes A (sea), B (seb), C (sec), and D (sed). It was found that 21% enterotoxigenic S. aureus harbored sea gene, 39% were carried seb gene, 37% were positive for sec-gene, and 3% were carried sed gene. None of all S. aureus strains harbored more than one of the enterotoxigenic genes. Based on the data obtained from the current study, it could be suggested that seb and sec genes are good candidates for the identification of S. aureus in clinical specimens. Further investigations are required to discover the association between these genes and the pathogenicity of this bacteria, and finally using these data in clinical settings.
- انتشار مقاله: 15-08-1398
- نویسندگان: Vahid Ghanbarinejad,Aboozar Kazemi,Mina Zareei,Reza Heidari,Hossein Hassanian-Moghaddam
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Protease,Bacillus sp,Moderately halophilic
- چکیده:
- چکیده انگلیسی: Screening and identification of moderately halophilic protease producing bacteria from different regions of Maharloo, a hypersaline lake in the southern area of Iran, were the objectives of this study. In the preliminary screening, 16 isolates exhibited proteolytic activity on saline skim milk agar. All isolates were able to grow comfortably in the media containing 7-15% of salt. Protease activity was assessed through in vitro colorimetric assays for general proteases and the strains were identified by 16S rDNA molecular marker. In comparison to Gram-negative bacteria, the Gram-positive rods, displayed higher proteolytic activities, and Bacillus sp. BCCS 041 was found as the highest protease producing strain with 0.43 U/ml of supernatant activity.
- انتشار مقاله: 29-11-1389
- نویسندگان: Younes Ghasemi,Sara Rasoul-Amini,Alireza Ebrahiminezhad,Aboozar Kazemi,Maryam Shahbazi,Najme Talebnia
- مشاهده