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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Helicobacter pylori,Gastritis,Polymorphism,IL-6,IL-18,TLR-4
- چکیده:
- چکیده انگلیسی: Background & Aims: Helicobacter pylori (H. pylori) infection is associated with peptic ulcer and gastric cancer. Polymorphisms of the genes coding Toll-like receptors (TLRs) may influence the innate and adaptive immune responses and affect the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expression in patients infected with H. pylori have remained unclear. The purpose of this study was to investigate the effect of TLR-4 Asp299Gly polymorphism on expression levels of IL-6 and IL-18 genes in patients infected with H. Pylori in comparison to uninfected patients.
Methods: In a case-control study, biopsies were collected from 58 H. pylori-infected and 44 uninfected subjects.Genotypes of TLR-4 Asp299Gly single-nuleotide polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The expression levels of mucosal IL-6 and IL-18 mRNA were measured by real time-PCR. Cytokine expression levels in the two groups were analysed using the t test.
Results: The frequencies of D/D and D/G genotypes were respectively 55.2% and 44.8% in H. pylori-infected and 77.2% and 22.8% in uninfected subjects. The expression level of IL-6 in H. pylori infected patients with TLR-4 gene polymorphism was significantly higher (P=0.001). However, no significant relationship between the expression level of IL-18 and TLR-4 gene polymorphism was reported in patients infected with H. pylori (P=0.419). In addition, no significant relationship between IL-6, IL-18 expression levels and TLR-4 gene polymorphism was reported in uninfected patients.
Conclusion: TLR-4 Asp299Gly Gene single-nuclotide polymorphism increases the expression levels of TL-6 and has an important role in development of Hopylori-associated gastrititis.- انتشار مقاله: 15-04-1394
- نویسندگان: Ghorbanali Rahimian,Nader Bagheri,Abolfazl Gholipour,Hedayatollah Shirzad
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Olive oil,Sesame oil,Synergism,Pseudomonas aeruginosa
- چکیده:
- چکیده انگلیسی: Background and aims: Nosocomial infections are common problems in developing and developed countries. One of the pathogens that causes these infections is Pseudomonas aeruginosa. According to the antibiotical resistance of this bacteria, It is essential to find new treatments like new herbal medicine. This study was aimed to determine in vitro antibacterial effects of sesame oil, olive oil and their synergism on P. aeruginosa.
Methods: In this experimental study, first, olive oil and sesame oil were obtained by cold-pressing assay. Then, P.aeruginosa ATCC 27853 was prepared from Iranian Research Organization for Science and Technology. Oils and their mixture with the same proportion were made in concentrations from 2 to 1024 mg/ml and the antibacterial character of these oils on P.aeruginosa was investigated by broth micro dilution method and then the amounts of the MIC and the MBC were demonstrated.
Results: The MIC of olive oil, sesame oil and their synergism on P.aeruginosa were reported; 16 mg/ml, 128 mg/ml and 128 mg/ml and the MBC of these oils were indicated; 64 mg/ml, 512 mg/ml and 512 mg/ml, respectively.
Conclusion: This study showed that sesame oil, olive oil and their synergism can inhibit bacterial growth. Olive oil has more antibacterial effect on this bacteria than sesame oil and their synergism. So, we conclude that there was no synergistic effect in these oils.- انتشار مقاله: 22-07-1395
- نویسندگان: Reza Heidari Soureshjani,Reza Alipoor,Seyed_Saadat Gholami,Reza Hashemi Hafshejani,Arefeh Kiyani,Yaghoob Madmoli,Abolfazl Gholipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,Olive oil,Sesame oil,Synergism
- چکیده:
- چکیده انگلیسی: Background and aims: Nosocomial infections are the main cause of the problems related to health and health care costs. One of the pathogens that causes these infections is Staphylococcus aureus. Its resistance to antibiotics forced researchers to find herbal substitution such as Sesame and Olive. This study was aimed to determine antibacterial effect of sesame oil, olive oil and their synergism on S. aureus.
Methods: In this experimental study, at first, olive oil and sesame oil was extracted by cold press assay and the mixture of them was prepared with equal proportion of both oils, and then the standard strain of lyophilized S. aureus ATCC 25923 was prepared from the Iranian Research Organization for Science and Technology. To determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oils, micro-broth dilution method was used.
Results: This study indicated the amounts of MIC for sesame oil, olive oil and their synergism; 32 mg/ml, 32 mg/ml, 8 mg/ml and the amounts of MBC; 128 mg/ml,
128 mg/ml, 32 mg/ml, respectively.
Conclusion: The results showed the same antibacterial activity for olive oil and sesame oil. It also showed that mixture of these oils enhances the growth inhibitory and bactericidal properties of the oils compared to using them individually.- انتشار مقاله: 12-06-1395
- نویسندگان: Reza Heidari Soureshjani,Zia Obeidavi,Vahid Reisi Vanani,Sepehr Ebrahimi Dehkordi,Neda Fattahyan,Abolfazl Gholipour
- مشاهده