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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Liver,NDV,Oncolytic Virus,azoxymethane
- چکیده:
- چکیده انگلیسی: Background: Treatment of cancer with chemo-radiotherapy causes severe side effects due to cytotoxic effects towards normal tissues which often results in morbidity. Therefore, developing anticancer agents which can selectively target the cancer cells and cause less side effects are the main objectives of the new therapeutic strategies for treatment advanced or metastatic cancers. Newcastle disease virus strains AF2240 and V4-UPM were shown to be cytolytic against various cancer cells in-vitro and very effective as antileukemicagents. Methods: 45 rats at 6 weeks of age, were randomly assigned to nine groups with 5 rats in each group, both azoxymethane (AOM) and 5-Fluorouracil (5-FU) were given to rats according to the body weight. NDV virus strains (AF2240 and V4-UPM) doses were determined to rats according to CD50 resulted from MTT assay. After 8 doses of NDV strians and 5-FU, tissue sections preparations and histopathological study of rats’ organs were done. Results: In this article morphological changes of rats’ organs, especially in livers, after treatment with a colon carcinogen (azoxymethane) and Newcastle disease virus strains have been recorded. We observed liver damage caused by AOM evidenced by morphological changes and enzymatic elevation were protected by the oncolytic viruses sections. Also we found that combination treatment NDV with 5-FU had greater antitumor efficacy than treatment with NDV or 5-FU alone. Conclusion: We noted morphological changes in liver and other rats’ organs due to a chemical carcinogen and their protection by NDV AF2240 and NDV V4-UPM seems to be most protective.
- انتشار مقاله: 11-04-1398
- نویسندگان: Rowa Mohammed Assayaghi,Aied Mohammed Alabsi,Gumballi Swethadri,Abdul Manaf Ali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,NDV strain AF2240,Flow-cytometry . glioblastoma multiforme
- چکیده:
- چکیده انگلیسی: Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest
of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells.
Methods: In this investigation, the proliferation of brain tumor cell line, glioblastoma multiform (DBTRG.05MG)
induced by NDV strain AF2240 was evaluated in-vitro, by using MTT proliferation assay. Furthermore, Cytological
observations were studied using fluorescence microscopy and transmission electron microscopy, DNA laddering in
agarose gel electrophoresis assay used to detect the mode of cell death and analysis of the cellular DNA content by
flowcytometery. Results: MTT proliferation assay, Cytological observations using fluorescence microscopy and
transmission electron microscopy show the anti-proliferation effect and apoptogenic features of NDV on DBTRG.05MG.
Furthermore, analysis of the cellular DNA content showed that there was a loss of treated cells in all cell cycle phases
(G1, S and G2/M) accompanied with increasing in sub-G1 region (apoptosis peak). Conclusion: It could be concluded
that NDV strain AF2240 is a potent antitumor agent that induce apoptosis and its cytotoxicity increasing while increasing
of time and virus titer.- انتشار مقاله: 05-04-1397
- نویسندگان: Rola Ali-Saeed,Aied M Alabsi,Aini Ideris,Abdul Rahman Omar,Khatijah Yusoff,Abdul Manaf Ali
- مشاهده