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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Aspergillus parasiticus,Listeria monocytogenes,Escherichia coli O157:H7,Candida parapsilosis,data organization method
- چکیده: زمینـه مطالعه: لاکتوباسیلها بعنوان منبعی ارزشمند برای ترکیبات ضد میکربی شناخته می شوند و از پتانسیل بالایی جهت محافظت بیـولـوژیـک غـذا در بـرابـر میکـروارگـانیسمهای مرتبط با آن برخوردارند. هدف: توان ضد میکربی 63 جدایه لاکتوباسیل لبنی علیه چهار میکروارگانیسم مهم مرتبط با غذا سنجیده شد. همچنین روش جدیدی به منظور سازماندهی دادههای حاصل از آزمون میکربی معرفی گردید که نتیجه آن مقایسهای مستدل تر و بر پایه اطلا عات بیشتر از حساسیت میکروارگانیسمهای شاخص به یک گروه از ترکیبات بود. ارتباط pH و خصوصیات ضد میکربی نیز سنجیده شد. روش کار: روش microbroth dilution assay جهت ارزیابی حساسیت میکروارگانیسمهای شاخص به سوپ رویی عاری از سلول کشت مایع (cell free culture supernatant; CFCS) لا کتوباسیلها مورد استفاده قرار گرفت. سازماندهی و پردازش نتایج حاصل از آزمونهای میکربی به هر دو روش سنتی ( بیان مقادیر حداقل غلظت ممانعت کننده رشد و حداقل غلظت کشنده) و روش پیشنهادی جدید که قدرت اثر مقایسهای هر نمونه CFCS علیه میکروارگانیسمهای شاخص مقایسه شونده را نشان می دهد، صـورت گـرفـت. نتـایـج: حسـاسیـت سـویـه های تست شده از ترتیب زیر تبعیت کرد: > L. monocytogenes > A. parasiticus > C. parapsilosis 7:H157.E. coli O لیستریا مونوسایتوژنز بر خلاف حساسیت بالا، بیشترین مقاومت را نسبت به مرگ در میـان میکـروارگـانیسـمهای آزمایش شده از خود نشان داد. میزان کارامدی CFCS لاکتوباسیلها در کشتن سویه های تست شده، ترتیب حساسیت زیر را نشان داد: > A. parasiticus > C. parapsilosis > L. monocytogenes 7:H157E. coli O. بین میزان pH وخصوصیت ضد میکربی ارتباط وجود داشت. PHهای CFCS غلیظ شده با مقادیر کمتر از 4 و 5/4 اثر مشخصی را به ترتیب بر روی حساسیت C. parapsilosis وE. coli داشتند. نتیجه گیری نهایی: توانایی لاکتوباسیلها در ممانعت از رشد میکروارگانیسمهای شـاخـص امیـدوار کننـده بـود و روش پیشنهـادی بـرای سـازمـانـدهـی دادههـا، بـا فـراهـم نمـودن اطلاعـات بیشتر موجب مقایسه دقیقتر میکروارگانیسمهای شاخص گردید.
- چکیده انگلیسی: BACKGROUND: Lactobacilli are known as a valuable source
of antimicrobial compounds and have a high potential of use in
food biopreservation against food related microorganisms.
OBJECTIVES: Antimicrobial potency of 63 dairy lactobacilli
isolates against four highly important food-related microorganisms
were evaluated. In addition, a new way in data organization was
introduced, which led to a more informative and rational
comparison of indicator microorganisms' susceptibilities to a set
of compounds. Correlation of pH and antimicrobial properties
was investigated. METHODS: Microbroth dilution assay was
used to evaluate indicator microorganisms' susceptibility to
lactobacilli CFCS (cell free culture supernatant). Results were
organized by both the conventional way - demonstrating the
minimum inhibitory and lethal concentrations of CFCS - and a
new suggested method, representing comparative effectiveness
of each CFCS specimen against indicator microorganisms of
comparison interest. RESULTS: Susceptibilities of tested strains
were in the following order: Escherichia coliO157:H7 > Listeria
monocytogenes > Aspergillus parasiticus> Candida parapsilosis.
Despite the high susceptibility of L. monocytogenes, it showed
the highest resistance to death among the tested microorganisms.
Eefficiency of Lactobacilli CFCS in killing the tested strains
showed the following susceptibility order: E. coli O157:H7 > A.
parasiticus> C. parapsilosis> L. monocytogenes. Antimicrobial
property was in correlation with the pH value of CFCS. PH had
a pronounced impact on susceptibilities of C. parapsilosisand E.
coli in pH values of concentrated CFCS lower than 4 and 4.5,
respectively. CONCLUSIONS: Potency of lactobacilli isolates in
growth inhibition of the indicator microorganisms was found
promising, and the suggested data organization method provided
additional information, leading to more precise comparison of
indicator microorganisms.- انتشار مقاله: 23-05-1392
- نویسندگان: Hadi Maleki,Ali Misaghi,Mohsen Amini,Abbas Saidi,Kambiz Akbari Noghabi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Transcription factor,sunflower,BZip,Osmotic stress,Patchy pattern
- چکیده:
- چکیده انگلیسی: Background: Sunflower (Helianthus annuus L.) is one of the important vegetable oil supplies in the world and in Iran, as well. It is classified as a drought semi-tolerant crop; however, its yield is adversely affected by drought stress. Understanding the initial events in sensing stress and the related physiologic and biochemical events thereafter, is crucial in designing drought stress breeding programs. Transcription factors are master molecules directly involved in the plant responses under drought stress, from signal perception and transduction to the regulation of physiologic processes.
Objective: The expression pattern of some bZip transcription factors in response to osmotic stress was investigated in sunflower.
Material and Methods: Employing real-time PCR to monitor, the response of 10 bZIP transcription factors was performed under different osmotic stress conditions including -0.3, 0.9, and 1.2 MPa. Whole seedling was sampled at 6, 12, and 24 h after the osmotic condition application.
Results: Exposure to osmotic potential of 0.9 MPa for 24 h caused a reduction in the fresh weight of the seedling. Among the evaluated genes, eight genes, bz-497, bz-502, bz-485, bz-499, bz-492, bz-504, bz-505, and bz-509 appeared as the osmotic stress responsive transcription factor. Changes in the expression of the genes under 0.3 MPa was observed for four genes. Most of the osmotic responsive genes appeared to be up-regulated. Most of responsiveness in the gene expression was happened under 0.9 MPa of the osmotic stress which is corresponding to fresh weight reduction in the seedlings. Among the investigated genes, two genes was identified to have possible roles in sensitive response of sunflower against drought stress.
Conclusions: It was a focus to have systemic view on the complex response of the plant to abiotic stress, and avoidance of the single gene analysis. Also, the importance of molecular data in molecular breeding procedures toward achievement of the stress tolerant lines was highlighted.- انتشار مقاله: 29-09-1394
- نویسندگان: Zeinab Esmailzadeh Mianlengeh,Masood Soltani Najafabadi,Abbas Saidi,Hossein Askari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Olive oil,Simple Sequence Repeats (SSRs),DNA fingerprinting,Genetic traceability
- چکیده:
- چکیده انگلیسی: A reliable DNA extraction method for use on extra virgin olive oil based on a commercial kit was defined, and the possibility of using this DNA for fingerprinting the original cultivar was demonstrated. The genetic traceability of single-cultivar virgin olive oil from two cultivars (Carolea and Frantoio) was achieved by identifying the varieties from which they were produced. This involved the analysis of DNA sequences using a panel of seven simple sequence repeats (SSRs) to provide genotype-specific allelic profiles. The amplified SSR fragments and the DNA profiles from the monovarietal oil corresponded to the profiles from the leaves of the same cultivar. The most reliable SSR in providing correct allele sizing in distinguishing either single-cultivar olive oil samples or the different ratios of their blends are DCA3, DCA4, DCA16, DCA17, and GAPU101, while DCA9, GAPU59 produced less concordance against data obtained by the genetic analysis of leaf samples. To have reproducible results, PCR product purification and selection of a set of markers with a highly robust amplification pattern is suggested.
- انتشار مقاله: 31-01-1394
- نویسندگان: Zohreh Rabiei,Sattar Tahmasebi Enferadi,Abbas Saidi,Sonia Patui,Gian Paolo Vannozzi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Proliferation,Alkaline phosphatase,Foeniculum vulgare,Osteogenesis
- چکیده:
- چکیده انگلیسی: Objective: Osteoporosis or silent disease is a major bone disorder in elderly women in current century. Estrogen has an important role in osteogenesis and prevention of bone fractures. Hormone replacement therapy (HRT) is usually accompanied by such effects as breast and ovary cancers. Thus, there is an increasing demand for replacement with plant phytoestrogens. This study is focused on determining the effects of Foeniculum vulgare extract on proliferation and osteogenesis progress in human mesenchymal stem cells.
Material and Methods: Human mesenchymal stem cells were isolated and treated with different amount of plant extracts (0.5 to 100 µg/ml). Extract cytotoxicity was measured using MTT assay. The alkaline phosphatase enzyme activity was measured to evaluate the differentiation progress.
Results: Results of MTT assay and alkaline phosphatase activity showed that Foeniculum vulgare extract, at range of 5 to 50 µg/ml, may positively affect cell proliferation and mineralization. The most proliferation and enzyme activity were seen with dose of 5 µg/ml.
Conclusions: Foeniculum vulgare has been used in Iranian folk medicine for many years. Our in vitro study showed that Foeniculum vulgare extract has osteoprotective effects.- انتشار مقاله: 21-03-1391
- نویسندگان: Zahra Mahmoudi,Masoud Soleimani,Abbas Saidi,Gholamreza Khamisipour,Arezoo Azizsoltani
- مشاهده