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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Colorectal cancer,Metastasis,siRNA,CD133
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ability. Objective: To investigate the anti-apoptotic and anti-angiogenic effect of CD-133 targeted siRNA in a colorectal cancer cell line. Methods: In this study, CD133-targeted siRNA transfection was conducted into HT-29 cells. MTT assay was employed to evaluate the cytotoxic effects of transfection on the cells. Flow cytometry was used to evaluate the apoptosis rate. The mRNA expression of apoptosis and metastasis related genes were assessed by quantitative Real-Time PCR (qRT-PCR). Wound healing assay was used to assess the migration potency of the infected cells. Results: Expression of CD133 was significantly downregulated after transfection of CD133-specific siRNA. Moreover, the rate of apoptosis was significantly increased after transfection. The migration potential of cells was diminished after transfection. siRNA delivery resulted in the modulation of expression of apoptosis and metastasis-related genes. Conclusion: siRNA mediated targeting of CD133 could be considered as a promising approach to treat CRC through suppressing the cancerous behavior of tumor cells.
- انتشار مقاله: 29-11-1397
- نویسندگان: Morteza Akbari,Dariush Shanehbandi,Milad Asadi,Navid Shomali,Afsaneh Faraji,Vahid Khaze,Abbas Pakdel,Ahad Mokhtarzadeh,Ali Asghar Ebrahimi,Aliakbar Shabani,Behzad Baradaran
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Beta-lactoglobulin,Adani goats,Alpha S1 casein,Gene expression, Mammary gland, Milk yield breeding value
- چکیده:
- چکیده انگلیسی: Background: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired.
Objectives: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed high and low breeding values.
Materials and Methods: The breeding values of the animals were estimated first by applying multi-trait random regression model. Using the biopsy gun, the mammary gland samples were taken and real-time PCR was applied to search the expression of the genes. Fixed factors of the model were the breeding value groups, sampling times and their interactions.
Results: The interactions were significant for both genes. At milking time, the high breeding value group exhibited more transcriptional levels for BLG and less transcriptional levels for CSN1S1 gene compared with the low breeding value group. The expression patterns of these genes were also different between the two breeding value groups. The maximum level of BLG and CSN1S1 transcriptions were found to occur at drying time.
Conclusions: A difference in the gene expression was observed between the two groups which indicate the change in the nucleotide sequence for transcription factor binding sites, or miRNA binding sites, otherwise in the coding regions. Therefore, the variations in the coding and promoter regions of this gene should be investigated in the further studies.
- انتشار مقاله: 03-12-1393
- نویسندگان: Salim Morammazi,Ali Akbar Masoudi,Rasoul Vaez Torshizi,Abbas Pakdel
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Design and Manufacturing Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: modal analysis,FEM Modeling,Ultrasonic Transducer,Young’s Modulus
- چکیده:
- چکیده انگلیسی: Ultrasonic transducers have found new applications such as ultrasonic assisted micromachining, micro forming, surface treatment, welding, etc. Apart from the transducer’s shape and size, the resonant frequencies and amplitude are seriously affected by materials properties used for transducer components. A further problem with the material is that their properties may vary from batch to batch and may also depend on the size of the raw stock. In this work using modal analysis, the material properties are calculated based on the frequency response method, which is more accurate than the nominal one. The finite element modelling was employed for both 2D and 3D FEM analysis to observe the behaviour of the cylindrical test rods and two sandwich-type piezoelectric transducers with the nominal frequency of 20 kHz and 30 kHz to find the validity of these properties. The obtained results showed that the modal analysis method could accurately determine the bar speed, Poisson's ratio and elastic modulus of the ultrasonic transducer components. The accuracy of this method increases by considering more vibration mode. Based on the results, obtained errors for FEM modelling of two ultrasonic transducers with the frequency of 20 kHz and 30 kHz are 0.15% and 0.33%, respectively.
- انتشار مقاله: 13-09-1397
- نویسندگان: Abbas Pak,Abbas Pak,Abbas Pak
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Design and Manufacturing Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: finite element method,Indentation Tube Forming,Intermittent Phenomenon,Ultrasonic Vibration
- چکیده:
- چکیده انگلیسی: Indentation forming is an internal tube forming process in which a mandrel with a diameter slightly larger than that of the tube is pressed inside the tube and in so doing, creates the internal profile. Forming forces have a significant effect on the spring back, residual stress, quality of the inner surface, quality of tube dimensions, and tool wear. In this study, the forming process of CK45 steel tube by carbide tungsten tool in the presence of ultrasonic vibration has been simulated and the effect of ultrasonic on the forming mechanism has been investigated by introducing two regimes according to the forming conditions. The effects of tool feed-speed and amplitude of vibration on forming force reduction have been investigated. According to the simulation results, the main reason for the force reduction in the presence of longitudinal tube ultrasonic vibration is the intermittent phenomenon which is the continuous or impulsive regime. The critical amplitude which determines the borderline of continuous and impulsive regimes is obtained 38µm by the simulation of the process. The maximum force reduction obtained in continuous regime is 64.2% at the critical amplitude. The simulation results are consistent well with the previous experimental data.
- انتشار مقاله: 09-03-1398
- نویسندگان: Saeid Dashti,Masih Paknejad,Amir Abdullah,Abbas Pak
- مشاهده