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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: vaccine,SLA,Leishmaniasis,Liposome,DDA,TDB
- چکیده:
- چکیده انگلیسی: Objective(s): Leishmaniasis is a complex parasitic disease that represents a major public health problem. Despite numerous attempts over the past decades, yet there is no effective vaccine against human leishmaniasis probably due to the lack of suitable adjuvants. In this study, a first generation liposomal-based Leishmania vaccine was developed using soluble Leishmania major antigens (SLA) and á, Ü-trehalose6, 6'- dibehenat (TDB) as an immunostimulatory adjuvant. In this liposome structure, the cationic lipid Dimethyldioctadecylammonium (DDA) provides intrinsic adjuvant activity and cholesterol was added as a membrane stabilizer. Liposomes containing SLA were prepared. Materials and Methods: BALB/c mice were subcutaneously (sc) immunized with Lip (DDA/TDB/CHOL)-SLA+, Lip (DDA/TDB)-SLA+, Lip (DDA)-SLA+, Lip (DDA/CHOL)-SLA+, SLA or Tris-HCl buffer. Immunization was done every two weeks for three weeks. The immunized mice were then challenged sc in the left footpad with 1×106 stationary phase L. major promastigotes (50 ìl), at 2 weeks after last booster injection.
Results: mice immunized with any of the liposomal formulations containing SLA (Lip-SLA+), substantially increased footpad swelling and parasite loads of foot and spleen with no significant difference compared to Tris-HCl buffer or SLA alone. Lip-SLA+ formulations induced a mixed Th1/Th2 immune response characterized by IFN-ã and IL-4 production as well as high levels of IgG1 anti-Leishmania antibody.
Conclusion: immunization with liposomes containing DDA and/or TDB in combination with SLA induces a mixed Th1/Th2 immune response and is not an appropriate strategy for preferential induction of a Th1 response and protection against leishmaniasis.- انتشار مقاله: 18-12-1395
- نویسندگان: Mansure Hojatizade,Ali Badiee,Ali Khamesipour,Abbas Mirshafiey,Javad Akhtari,Ahmad Mehravaran,Seyedeh Hoda Alavizadeh,Azam Abbasi,Zahra Saberi,Amin Reza Nikpoor,Mahmoud Reza Jaafari
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: vaccine,SLA,Leishmaniasis,Liposome,DDA,TDB
- چکیده:
- چکیده انگلیسی: Objective(s): Leishmaniasis is a complex parasitic disease that represents a major public health problem. Despite numerous attempts over the past decades, yet there is no effective vaccine against human leishmaniasis probably due to the lack of suitable adjuvants. In this study, a first generation liposomal-based Leishmania vaccine was developed using soluble Leishmania major antigens (SLA) and á, Ü-trehalose6, 6'- dibehenat (TDB) as an immunostimulatory adjuvant. In this liposome structure, the cationic lipid Dimethyldioctadecylammonium (DDA) provides intrinsic adjuvant activity and cholesterol was added as a membrane stabilizer. Liposomes containing SLA were prepared. Materials and Methods: BALB/c mice were subcutaneously (sc) immunized with Lip (DDA/TDB/CHOL)-SLA+, Lip (DDA/TDB)-SLA+, Lip (DDA)-SLA+, Lip (DDA/CHOL)-SLA+, SLA or Tris-HCl buffer. Immunization was done every two weeks for three weeks. The immunized mice were then challenged sc in the left footpad with 1×106 stationary phase L. major promastigotes (50 ìl), at 2 weeks after last booster injection.
Results: mice immunized with any of the liposomal formulations containing SLA (Lip-SLA+), substantially increased footpad swelling and parasite loads of foot and spleen with no significant difference compared to Tris-HCl buffer or SLA alone. Lip-SLA+ formulations induced a mixed Th1/Th2 immune response characterized by IFN-ã and IL-4 production as well as high levels of IgG1 anti-Leishmania antibody.
Conclusion: immunization with liposomes containing DDA and/or TDB in combination with SLA induces a mixed Th1/Th2 immune response and is not an appropriate strategy for preferential induction of a Th1 response and protection against leishmaniasis.- انتشار مقاله: 18-12-1395
- نویسندگان: Mansure Hojatizade,Ali Badiee,Ali Khamesipour,Abbas Mirshafiey,Javad Akhtari,Ahmad Mehravaran,Seyedeh Hoda Alavizadeh,Azam Abbasi,Zahra Saberi,Amin Reza Nikpoor,Mahmoud Reza Jaafari
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Leukemia,growth factor,Vascular Endothelial
- چکیده:
- چکیده انگلیسی: Background and Objective: Vascular endothelial growth factor (VEGF) is a cytokine which is overexpressed in many malignant cancers including leukemia. VEGF plays an important role in tumor invasion and metastasis. Determination of the pattern of VEGF expression in human leukemic cell lines could be useful not only in screening of new antileukemic agents but also to study the mechanism of their action. In the present study, the pattern of VEGF production in some human leukemic cell lines has been assessed in vitro. Materials & Methods: Three human leukemic cell lines (Molt-4, Jurkat and U937) were used in this study. The cells were cultured in complete RPMI medium and then incubated with different concentrations of phorbolmyristate acetate (PMA) or phytoheamagglutinin (PHA) for 48 hours. The level of VEGF secreted in the cellculture supernatants were measured with enzyme-linked immunosorbent assay kits (R and D systems). Results: U937 cells produced a large amount of VEGF without any stimulus and PHA/PMA did not show any substantial effect on VEGF production by U937 cells. However, Molt-4 and Jurkat cells, produced VEGF less than U937 when cultured alone (with no stimulation) and PMA/PHA significantly increased the VEGF production in these cells dose-dependently. Conclusion: Different patterns of VEGF expression were shown in different leukemic cell lines (Molt-4, Jurkat and U937) used in this study. These cells could be useful tools for screening of VEGF modulators and so for planning of new drugs for treatment of leukemia and other VEGF related disorders.
- انتشار مقاله: 29-04-1393
- نویسندگان: Fatemeh Hajghasemi,Abbas Mirshafiey
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Vitamin D,Advanced Glycation End Products,Cholecalciferol,Diabetes Complications,Hexosamine pathway
- چکیده:
- چکیده انگلیسی: Objective(s): The aim of this study was to investigate the effect of vitamin D on glucose metabolism, as well as the expression of five key genes involved in the development of diabetes complications in liver tissue of diabetic rats.
Materials and Methods: Twenty-four male Sprague–Dawley rats were randomly divided into three groups (8 rats in each group). The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin to develop diabetes. Groups were treated for four weeks either with placebo or vitamin D (two injections of 20000 IU/kg). Thereafter, serum levels of glucose, insulin and HbA1c were assessed. Liver tissue was examined for the level of advanced glycation end products (AGEs) and the gene expression of AGE cellular receptor (AGER), glyoxalase-1 (GLO-1), aldose reductase (AR), O-linked N-acetylglucosamine transferase (OGT) and glutamine/ fructose-6-phosphate aminotransferase (GFAT).
Results: Vitamin D injection resulted in a significant increase in plasma level of 25-hydroxycholecalciferol, which could improve hyperglycemia about 11% compared to placebo-receiving diabetic rats (P=0.005). Insulin level increased as a result of vitamin D treatment compared to control (3.31±0.65 vs. 2.15±0.79; P= 0.01). Serum HbA1c and liver AGE concentrations had a slight but insignificant reduction following vitamin D intake. Moreover, a significant decline was observed in gene expression of AGER and OGT in liver tissue (P=0.04 and PConclusion: Vitamin D might contribute in ameliorating diabetes complications not only by improving blood glucose and insulin levels, but also by suppressing AGER and OGT gene expression in the liver.- انتشار مقاله: 15-08-1397
- نویسندگان: Hoda Derakhshanian,Mahmoud Djalali,Mohammad Hassan Javanbakht,Ehsan Alvandi,Mohhamd Reza Eshraghian,Abbas Mirshafiey,Hoda Nadimi,Samane Jahanabadi,Mahnaz Zarei,Abolghassem Djazayery
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Vitamin D,Advanced Glycation End Products,Cholecalciferol,Diabetes Complications,Hexosamine pathway
- چکیده:
- چکیده انگلیسی: Objective(s): The aim of this study was to investigate the effect of vitamin D on glucose metabolism, as well as the expression of five key genes involved in the development of diabetes complications in liver tissue of diabetic rats.
Materials and Methods: Twenty-four male Sprague–Dawley rats were randomly divided into three groups (8 rats in each group). The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin to develop diabetes. Groups were treated for four weeks either with placebo or vitamin D (two injections of 20000 IU/kg). Thereafter, serum levels of glucose, insulin and HbA1c were assessed. Liver tissue was examined for the level of advanced glycation end products (AGEs) and the gene expression of AGE cellular receptor (AGER), glyoxalase-1 (GLO-1), aldose reductase (AR), O-linked N-acetylglucosamine transferase (OGT) and glutamine/ fructose-6-phosphate aminotransferase (GFAT).
Results: Vitamin D injection resulted in a significant increase in plasma level of 25-hydroxycholecalciferol, which could improve hyperglycemia about 11% compared to placebo-receiving diabetic rats (P=0.005). Insulin level increased as a result of vitamin D treatment compared to control (3.31±0.65 vs. 2.15±0.79; P= 0.01). Serum HbA1c and liver AGE concentrations had a slight but insignificant reduction following vitamin D intake. Moreover, a significant decline was observed in gene expression of AGER and OGT in liver tissue (P=0.04 and PConclusion: Vitamin D might contribute in ameliorating diabetes complications not only by improving blood glucose and insulin levels, but also by suppressing AGER and OGT gene expression in the liver.- انتشار مقاله: 15-08-1397
- نویسندگان: Hoda Derakhshanian,Mahmoud Djalali,Mohammad Hassan Javanbakht,Ehsan Alvandi,Mohhamd Reza Eshraghian,Abbas Mirshafiey,Hoda Nadimi,Samane Jahanabadi,Mahnaz Zarei,Abolghassem Djazayery
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: TLR4,IL-6,TLR2,alfa-L-Guluronic acid,G2013,CVID,NF-B,IL-1
- چکیده:
- چکیده انگلیسی: Background: Common variable immunodeficiency (CVID) is a primary immune disorder associated with hypogammaglobulinemia, recurrent infections and autoimmune diseases. CVID patients are frequently in contact with infectious pathogens leading to the activation of innate immunity through Toll-like receptors (TLR) affecting adaptive immunity. The aim of the present study was to test the immunomedulatory effect of small molecule G2013, a novel designed non-steroidal anti-inflammatory agent in CVID.
Materials and Methods: After blood sampling from 16 CVID patients and 16 age- and sex-matched healthy controls, peripheral blood mononuclear cells (PBMCs) were isolated and treated with/without lipopolysaccharide (LPS), lipopolyteichoic acid (LTA), and G2013. Assessing the immunomodulatory effect of G2013, flowcytometry was done for quantify the protein expression of TLR2 and TLR4. Gene expressions of signaling molecules involved in the TLR2 and TLR4 pathways were assessed by real-time PCR. ELISA performed assessing the production of IL-1b and IL-6.
Results: G2013 significantly decreased the intensity of TLR2 expression in CVID PBMCs (p=0.001) also G2013 decreased significantly the NF-kB gene expression in PBMCs of CVID patients (p=0.006).
Conclusion: These results indicated that G2013 had immunomodulatory effect at least in part via TLR2 and NF-kB expression. G2013 by decreasing MFI of TLR2 expression and NFkB gene expression provide the possibility of designing new drugs for preventing or controlling autoimmunity in CVID patients.- انتشار مقاله: 23-02-1396
- نویسندگان: Laleh Sharifi,Asghar Aghamohammadi,Monireh Mohsenzadegan,Nima Rezaei,Farzaneh Towfighi Zavareh,Mona Moshiri,Saied Bokaie,Anis Barati,Seyed Javad Sayedi,Gholamreza Azizi,Abbas Mirshafiey
- مشاهده