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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Nanoparticles,ICD-85,HeLa Cell Line,MTT assay,Caspase-8
- چکیده:
- چکیده انگلیسی: The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cellscompared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the encapsulation of ICD-85 enhances its anti-proliferative effects. Taken together, these results suggest that the delivery of ICD-85 in nanoparticles may be a promising approach for the treatment of the cancer.
- انتشار مقاله: 10-10-1390
- نویسندگان: Saeed Moradhaseli,Abbas Mirakabadi,Ali Sarzaeem,Morteza Kamalzadeh,Reza Haji Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR-RFLP,Genotypes,Alpha 1-antitrypsin deficiency,Phenotypes,IEF
- چکیده:
- چکیده انگلیسی: Alpha 1-antitrypsin (AAT) or alpha 1-protease inhibitor (PI) is the principal inhibitor of proteolytic enzyme in serum. Its phenotypic variability has been reported to be associated with liver, lung diseases and rheumatoid arthritis in humans. There is much documentation about high risk phenotypes of PI in some regions of the world, however, there are no reliable reports on these phenotypes and genotypes and their related diseases in Iranian population. The aim of this study was to determine PI phenotypes and genotypes in Iranian patients suffering from PI deficiency. For this purpose, whole blood samples from 307 patients suspected of diseases
related to PI deficiency, and 156 healthy persons were examined. PI phenotypes and genotypes were determined by isoelectric focusing (IEF) and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP), respectively. Allele frequencies from patients and normal subjects were compared. For reliability, a family study of the patients was also carried out. The PI phenotype frequencies of all six possible combinations of M, S and Z haplotypes in patients were: MM, 77.20%; MS, 6.18%; MZ, 7.17%; SS,
3.91%; ZZ, 4.56%; SZ, 0.98% and in normal subjects were: MM, 78.20%; MS, 5.76%; MZ, 15.38%; SS, 0.64%; 0% for ZZ and SZ. Analysis of data showed that there was a significant difference between patients (with liver, lung diseases and rheumatoid arthritis) and control subjects (p< 0.05). In Conclusion, the allelic frequencies of S and Z in the patient group were 7.49% and 8.63%, while in the normal subjects
were 5.13% and 4.17%, respectively. This is the first report of the prevalence of high risk alleles (Z and S) in patients suspected of PI deficiency and related diseases in Iran.- انتشار مقاله: 09-07-1384
- نویسندگان: Abbas S. Lotfi,Seyed Alireza Mesbah-Namin,Bahman GH Goudarzi,Abbas Zare Mirakabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Purification,Iranian snake venom,Agkistrodon halys,Thrombin-like enzyme,Coagulant activity
- چکیده:
- چکیده انگلیسی: The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel filtration on Sephadex G-50, ion-exchange chromatography on DEAE-Sepharose and HPLC with a C18 column. A trial for the purification of protease resulted in an enzyme with specific activity of 721.2 (μmol/min/mg), which was purified by 72.1 fold. The purified thrombin-like enzyme designated AH144 was found to have a molecular weight of approximately 30.5 kDa. This thrombin-like enzyme had the highest activity at 37 °C and pH 7.5. Enzyme activity increased as its concentration increased, and the purified enzyme did not have any effect on casein. AH144 demonstrated clotting and proteolytic activities in the presence of the human plasma and the synthetic substrate (BApNA), respectively. Data emphasized the possibility of AH144 for quantitative determination of fibrinogen.
- انتشار مقاله: 30-02-1388
- نویسندگان: Mohammad Ghorbanpour,Farzin Zokaee Ashtiani,Abbas Zare Mirakabadi,Hosein Zolfagharian
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Hemiscorpius lepturus,Gloydius Halys,ICD-85
- چکیده:
- چکیده انگلیسی: Background: Breast cancer is now the most important type of cancer in women around the globe and accounts for 25% of all types of cancer. Prevention and treatment of cancer are essential.
Method: The main methods for treating cancer include chemotherapy, surgery, radiotherapy, gene therapy, and hormone therapy. Chemopreventive test programmes began in 1987, when over 1,000 agents and agent combinations were selected and evaluated in preclinical studies of chemopreventive activity against various types of cancers.
Results: An important feature of anticancer drugs is a cytotoxic effect on cancer cells; these drugs have some cytotoxic agents found in animal venom. The ICD-85 is a combination of three peptides, ranging from 10,000 to 30,000 Da, and derived from the venom of the Iranian brown snake (Gloydius halys) and the yellow scorpion (Hemiscorpius lepturus).
Conclusion: ICD-85 has an anti-proliferative effect and anti-angiogenesis activity on cancer cells. The side effects of chemotherapy are multiple drug resistance and effects on natural tissues, among others. Therefore, cytotoxic anticancer drugs are useful in treating cancer. The present work investigates the effects of ICD-85 on in vivo and in vitro studies.- انتشار مقاله: 10-05-1398
- نویسندگان: Mohammad Reza Zinatizadeh,Abbas Zare Mirakabadi,Peyman Kheirandish Zarandi,Hamid Reza Mirzaei,Farzaneh Parnak,Sholeh Javadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی: Scorpion,Antisera,Apistobuthus,Buthacus,Vachoniolus
- چکیده:
- چکیده انگلیسی: Background: Scorpion sting is a significant health problem in southern provinces of Iran. Many thousands of people are stung by different species of scorpions annually. More than 60 scorpion species have been reported to be present in Iran. However, Razi Institute manufactures polyvalent antivenom against only six species of scorpions, excluding Buthacus macrocentrus, Apistobuthus susanae and Vachoniolus iranus, wide spread scorpion species in the south region of Iran. Since the venom of these scorpion species is not included in the production of Razi polyvalent antivenom, the aim of this study was to check the neutralization capacity of our antivenom against these species.
Methods: In this research, after collecting scorpions (at night), milking by the electroshock method, the venom was lyophilized and the LD50 was determined. The fractions were then separated by gel chromatography and HPLC. Using SDS page electrophoresis, the fractional molecular weight was determined. At the end, the potency test of these scorpion venoms was carried out in the vicinity of the antiserum produced by Razi Institute on the animal. In the present work, we tried to investigate the cross reactivity of present antivenom against these excluded scorpion species.
Results: The antisera production of Razi Institute was able to neutralize the 33 LD50 of Apistobuthus susanae venom.This antivenom could neutralize Buthacus macrocentrus as well as Vachoniolus iranus scorpion venoms by 41 LD50 and 15 LD50, respectively.
Conclusion: Based on the results obtained in the present study that indicate the neutralization of the three species of scorpions’ venom, not included in antivenom production, the present Razi polyvalent antivenom is able to be used in patients stung by these 3 species of scorpions.- انتشار مقاله: 27-03-1398
- نویسندگان: Bahzad Masihipour,Abbas Zare Mirakabadi,Mehdi Kheirollahpour,Hadi Rabiei,Ali Hedayat
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 25-02-1395
- نویسندگان: Mahboobeh Balali Bahadorani,Abbas Zare Mirakabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی: Snake venom,Cytotoxicity effect,HEK-293,Acute renal failure,Macrovipera lebetina
- چکیده:
- چکیده انگلیسی: Background:Envenomation by Macrovipera lebetina (M. lebetina) is characterized by prominent local tissue damage, hemorrhage, abnormalities in the blood coagulation system, necrosis, and edema. However, the main cause of death after a bite by M. lebetina has been attributed to acute renal failure (ARF). It is unclear whether the venom components have a direct or indirect action in causing ARF. To investigate this point, we looked at the in vitro effect of M. lebetina crude venom, using cultured human embryonic kidney (HEK-293) mono layers as a model.
Methods: The effect of M. lebetina snake venom on HEK-293 growth inhibition was determined by the MTT assay and the neutral red uptake assay. The integrity of the cell membrane through LDH release was measured with the Cytotoxicity Detection Kit. Morphological changes in HEK-293 cells were also evaluated using an inverted microscope.
Results: In the MTT assay, crude venom showed a significant cytotoxic effect on HEK-293 cells at 24 hours of exposure and was confirmed by the neutral red assay. Also, at 24 hours exposure, crude venom caused a non-significant increase in LDH activity of the culture medium at concentrations above 20 μg/ml. Various morphological abnormalities were observed in cells exposed to the venom and showed loss of their common polygonal shape, appearing as several roughly rounded cells of variable size. The M. lebetina crude venom induced detachment of cells from the plate.
Conclusion: Based on the results obtained in this study, it can be concluded that the Iranian snake M. lebetina venom causes a cytotoxic effect on kidney tissue not by necrotic mechanism but rather by secondary effects, including hypotension, hemolysis, hemoglobinuria, rhabdomyolysis, myoglobinuria and disseminated intravascular coagulation (DIC), which may lead to ARF.- انتشار مقاله: 29-09-1394
- نویسندگان: Hourieh Esmaeili Jahromi,Abbas Zare Mirakabadi,Morteza Kamalzadeh
- مشاهده