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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: gene polymorphism,Oral pre cancer and cancer,CYP1A1,PCR-RFLP
- چکیده:
- چکیده انگلیسی: Background: The aim of this study was to evaluate any association between CYP1A1 (T6235C and C4887A,
A4889G) gene polymorphisms and the risk of oral pre-cancer and cancer. Methods: In the present study, 250 patients
with oral pre-cancer and/or cancer and 250 healthy controls were genotyped for CYP1A1 T6235C, C4887Aand A4889G
polymorphisms by the PCR-RFLP method. Results: None of the CYP1A1 polymorphisms were associated with the risk of
either oral cancer or pre cancer. Nor were any links with clinical parameters of oral cancer found. However, among the
consumers of areca nut/pan masala the TC, CA and AG genotypes respectively for the CYP1A1 T6235C,C4887Aand
A4889G polymorphisms were significantly more frequent in controls compared to cases ( p values for cases vs. controls
of 0.0032, 0.0019 and 0.0009, respectively). Similarly, compared to the haplotype TCA, TAG constituted by CYP1A1
T6235C and C4887A and A4889G was more common in controls (6.88%) than in cases (4.07%). Conclusion: Our
results suggest that genotypes regarding CYP1A1 polymorphisms may modulate the risk of oral cancer and pre-cancer
among the areca nut/pan masala consumers. The haplotype may also exert an influence in our north Indian population.- انتشار مقاله: 28-04-1396
- نویسندگان: Kumud Nigam,Somali Sanyal,Shalini Gupta,Om Prakash Gupta,Abbas Ali Mahdi,M L B Bhatt
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Prostate cancer,MicroRNA,BPH,Biomarkers
- چکیده:
- چکیده انگلیسی:
Objective: MicroRNAs (miRs) are class of small non-coding regulatory RNA aberrantly expressed in various types of malignancies including prostate cancer and serves as potential targets to develop new diagnostic and therapeutic strategies. In this quiet we investigated miRNAs expression profile in benign prostatic hyperplasia (BPH) and prostate cancer (PCa) tissue samples and correlated their expression with clinicopathological parameters. Methodology: The miRNAs expression profile as well as their validation has been done by using Microarray and RT-PCR, respectively. Additionally, we also tried to speculate microRNA-mRNA regulatory module through computational target predictions by using Targetscan, Miranda and MirWalk and obtained results were analysed through DAVID software. Result: We observed that miR-711 is significantly deregulated in BPH and PCa, compared to controls. The lower expression of miR-711 was found to be significantly associated with high Gleason score and metastatic disease. Furthermore, the computational target prediction analysis explored miR-711 association to various cancer cells signalling cascade key molecules associated with cancer cell survival.Conclusion: From our observations we suggest that miR-711 may play a critical role in PCa progression, regulation of various cancer cell survival signalling cascades and that it may be a valuable biomarker for prediction of metastatic disease and poor prognosis in PCa.- انتشار مقاله: 06-02-1396
- نویسندگان: Mohammad Waseem,Mohammad Ahmad,Vikas Srivastava,Namrata Rastogi,Mohammad Serajuddin,Shashank Kumar,Durga Mishra,Satya Narain Sankhwar,Abbas Mahdi
- مشاهده